Publications by authors named "Poque S"

Article Synopsis
  • * Using various processing techniques, we discovered an ideal mix of extracts that boosted root growth in Arabidopsis by 25% and cut down on fertilizer needs by the same amount when applied to tomato plants.
  • * Our analysis showed that this process not only enhances plant nutrient metabolism but also produces leftover biomass that could be used in a biorefinery for other products, improving cost-effectiveness.
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Drought is a major environmental stressor that limits seedling growth. Several studies have found that some ectomycorrhizal fungi may increase the drought tolerance of nursery-raised seedlings. However, the precise role that different ectomycorrhizal fungi species play in drought tolerance remains unclear.

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Article Synopsis
  • Mixed virus infections in crops, specifically potato virus A (PVA) and potato virus X (PVX), lead to increased severity of symptoms and impaired plant growth due to viral synergism, which is more severe than individual infections.
  • Research combining image-based phenotyping and metabolite analysis revealed that co-infected plants experienced stress, shown by increased leaf temperature and decreased photosynthesis, while single infections maintained better photosynthetic activity.
  • Over 200 metabolites were affected during mixed infections, particularly those related to defense and metabolic processes, indicating a significant increase in oxidative stress and a decline in the plant's ability to cope with this stress compared to single infections.
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Sweet potato virus disease (SPVD), caused by synergistic infection of (SPCSV) and (SPFMV), is responsible for substantial yield losses all over the world. However, there are currently no approved treatments for this severe disease. The crucial role played by RNase III of SPCSV (CSR3) as an RNA silencing suppressor during the viruses' synergistic interaction in sweetpotato makes it an ideal drug target for developing antiviral treatment.

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Article Synopsis
  • Sweet potato chlorotic stunt virus (CSR3) uses its class 1 ribonuclease III to suppress RNA silencing in plants by cleaving essential small interfering RNAs, aiding its survival and reducing crop yields significantly.
  • A new high-throughput screening (HTS) assay utilizing fluorescence resonance energy transfer (FRET) was developed to identify potential inhibitors of CSR3, testing 6,620 compounds and discovering 109 potential inhibitors.
  • The three best inhibitors were validated, and a parallel screen for a similar enzyme from Escherichia coli showed that CSR3 and EcR3 are inhibited by different types of molecules, suggesting the HTS method's broad applicability in discovering inhibitors for class 1 RNase III enzymes
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Background: Virus diseases caused by co-infection with (SPFMV) and (SPCSV) are a severe problem in the production of sweetpotato ( L.). Traditional molecular virus detection methods include nucleic acid-based and serological tests.

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The viral infection process is a battle between host defense response and pathogen antagonizing action. Several studies have established a tight link between the viral RNA silencing suppressor (RSS) and the repression of salicylic acid (SA)-mediated defense responses, nonetheless host factors directly linking an RSS and the SA pathway remains unidentified. From yeast two-hybrid analysis, we identified an interaction between the potyviral RSS helper-component proteinase (HCPro) and SA-binding protein SABP3.

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Background: Sharka is caused by Plum pox virus (PPV) in stone fruit trees. In orchards, the virus is transmitted by aphids and by grafting. In Arabidopsis, PPV is transferred by mechanical inoculation, by biolistics and by agroinoculation with infectious cDNA clones.

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Sharka is a devastating viral disease caused by the Plum pox virus (PPV) in stone fruit trees and few sources of resistance are known in its natural hosts. Since any knowledge gained from Arabidopsis on plant virus susceptibility factors is likely to be transferable to crop species, Arabidopsis's natural variation was searched for host factors essential for PPV infection. To locate regions of the genome associated with susceptibility to PPV, linkage analysis was performed on six biparental populations as well as on multiparental lines.

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