Comparison of discriminant methods and deep learning analysis in plant taxonomy: a case study of Elatine.

Elatine is a genus in which, flower and seed characteristics are the most important diagnostic features; i.e. seed shape and the structure of its cover found to be the most reliable identification character.

Formalized classification of ephemeral wetland vegetation (Isoëto-Nanojuncetea class) in Poland (Central Europe).

Formalized classification of the class Isoëto-Nanojuncetea has not been performed in Poland. We used 69,562 relevés stored in Polish Vegetation Database. Based on the literature and expert knowledge we selected 63 diagnostic species for the Isoëto-Nanojuncetea class.

Rediscovery of (Cyperaceae) on the northeast periphery of its range in Europe.

In recent years, three large populations of were found in Poland, the richest occurrence of this species in over 30 years. The goal of this research is to determine the habitat factors lead to the mass occurrence of and the present situation of that species and its habitat in Central Europe. Soil conditions of the three populations were studied.

Resurrection and typification of (Elatinaceae), a long-forgotten waterwort species.

The name Seub. is generally treated as one of the synonyms of Guss. However, recent morphological, phylogenetic and karyological studies indicate that this judgement should be revised.

Seed morphometric characteristics of European species of (Elatinaceae).

L. contains ca. 25 small, herbaceous, annual species distributed in ephemeral waters in both hemispheres.

Molecular phylogenetics, seed morphometrics, chromosome number evolution and systematics of European L. (Elatinaceae) species.

The genus contains 25 species, all of which are small, herbaceous annuals distributed in ephemeral waters on both hemispheres. However, due to a high degree of morphological variability (as a consequence of their amphibious life-style), the taxonomy of this genus remains controversial. Thus, to fill this gap in knowledge, we present a detailed molecular phylogenetic study of this genus based on nuclear (rITS) and plastid (, , ) sequences using 27 samples from 13 species.

Flood induced phenotypic plasticity in amphibious genus Elatine (Elatinaceae).

Vegetative characters are widely used in the taxonomy of the amphibious genus Elatine L. However, these usually show great variation not just between species but between their aquatic and terrestrial forms. In the present study we examine the variation of seed and vegetative characters in nine Elatine species (E.

Assessment of ER-alpha and ER-beta expression levels in malicious tumors of the uterine corpus.

Purpose: The purpose of this article is to present an assessment of the expression levels of estrogen receptors ER-alpha and ER-beta in malicious tumors of the uterine corpus.

Material And Methods: Estrogen receptor expression levels were tested using semiquantitative immunohistochemical methods. Paraffin-embedded sections of tissue from the corpus of the uterus from 171 patients were used in the research.

Type IV collagen and CD44v6 expression in benign, malignant primary and metastatic ovarian tumors: correlation with Ki-67 and p53 immunoreactivity.

Objective: Loss of basement membrane (BM) components, such as type IV collagen has been demonstrated in ovarian cancer, but the associations with other molecules like CD44v6, involved in metastatic process of ovarian carcinoma, have not been fully analyzed. This study investigates the expression of type IV collagen, CD44v6 molecule in correlation with p53 and Ki-67 presence in primary and metastatic lesion of ovarian carcinoma to define their role in metastases of ovarian carcinoma.

Methods: The expression of type IV collagen, CD44v6, p53, and Ki-67 was evaluated on frozen tissue sections from primary ovarian tumors (malignant n = 37, benign n = 16), metastatic lesions (n = 29) and ascitic fluid cells (n = 28).

The meaning of the methylation of genomic DNA in the regulation of gene expression levels.

Introduction: Methylation of genomic DNA is one of the major mechanisms that deactivates genes and regulates their tissue-specific transcription levels. Its patterns are based on clonal inheritance that occurs in the early stages of embryogenesis. All changes in the DNA methylation levels occurring especially in the promoter region of the genes, which involve hypo- as well as hyper-methylation, lead to cell differentiation and growth disorders.

Expression of high molecular weight cysteine proteinase inhibitor in ovarian cancer tissues: regulation of cathepsin B expression by placental CPI.

Mouse polyclonal antibodies against placental cysteine proteinase inhibitor (CPI) react with the placental 67 kDa CPI on Western blots, and CPI present in ovarian cancer homogenate and serum was shown by double immunodiffusion to react with the same antiserum. By immunohistochemical staining, positive expression of high molecular weight CPI was observed on the tumour cell surface in serous and endometrioid ovarian carcinomas with metastasis. Normal endometrioid tissue was not stained with anti-placental CPI antibodies.

Environmental factors and risk for endometrial cancer development.

Getting to know the most important environmental risk factors for endometrial cancer development lets us provide well programmed primary preventive activity, thus avoiding or greatly reducing environmental risk factors for diseases. This activity may lead to a reduced number of new cases of endometrial cancer. We can help women live longer and better by well programmed preventive activity even when medical knowledge has reached such a high level of development.

[Estimation of DNA methylation level in endometrial cancer tissues].

Objectives: A level of DNA methylation plays an important role in regulation of cellular gene's expression. Estimation of DNA methylation level in endometrial neoplastic tissues compared to normal endometrial samples was the aim of this study.

Design: It was to be shown, that changes in methylation rate in promotory regions could lead to carcinogenesis in particular cell.

[Estimation of DNA methylation level in nonendometrial uterus malignancies].

Objectives: Rebuilding of genome structure leads to many pathological states including neoplastic malignancies. Rebuilding often occurs as a process caused by disturbances in gene silencing mechanism. DNA methylation pattern is one of the most important mechanisms connected to gene's silencing.

[Gastric cancer in pregnancy--a case report].

Authors describe a case of gastric carcinoma diagnosed during 27th week of pregnancy in 27-year-old woman with mild gastrointestinal symptoms in second and third trimester accompanied with severe anemia. Gastrofiberoscopy with a biopsy confirmed a diagnosis of diffused gastric adenocarcinoma. Sudden symptoms of premature labour in 33-rd week of pregnancy finished with a premature labor.

[Choriocarcinoma with atypical clinical course. Case report].

Authors describe a case of choriocarcinoma with an atypical clinical course in 28-year old woman nine months after a normal pregnancy and labour. Diagnosis of the disease was very hard because endometrial biopsy did not show any suspected cells and two performed pregnancy tests were not positive. Diagnosis was confirmed after a hysterectomy.

[Evaluation of expression and correlation between P53, BCL-2 and BAX proteins in ovarian neoplasms].

Objectives: Interactions between BCL-2 and other proteins regulating programmed cells death in ovarian carcinomas are poorly understood.

Design: The evaluation of expression of P53, BCL-2 and BAX proteins in ovarian carcinomas. The associations between oncoproteins studied and the histological structure, grade of differentiation and stage of disease were also analysed.

Expression and mutation of p53 in tumor effusion cells of patients with ovarian carcinoma: response to cisplatin-based chemotherapy.

p53 alterations are considered as one of the most important factors responsible for drug resistance in ovarian carcinomas, although the relationship between p53 gene status and response to cisplatin-based chemotherapy in ovarian cancer patients remains unclear. The aim of the study was to evaluate the relationship between p53 protein accumulation, p53 gene mutation and response to cisplatin-based chemotherapy in patients with ovarian carcinoma considering conventional clinicopathological parameters. Tissue sections and corresponding cyst and/or ascitic fluid cells from 79 patients with epithelial ovarian cancer were analyzed immunohistochemically for p53 expression.

[Comparative clinical analysis of cesarean section technique by Misgav Ladach method and Pfennenstiel method].

Clinical and biochemical parameters were analysed in 55 patients who underwent a caesarean section performed using Misgav Ladach method compared to reference group of 41 patients who underwent caesarean section using Pfannenstiel method. Shortened operation time, shortened hospitalisation time and less postoperative morbidity were observed in the Misgav Ladach group. This kind of method seems to have advantages in comparison to Pfannenstiel method.

Factors influencing period of time between negative second-look laparotomy and ovarian carcinoma recurrence. Multicenter study in Poland.

Objective: The aim of our study was to evaluate factors such as primary clinical stage, presence of ascites, serum CA 125 antigen level, histological type of ovarian cancer, cell differentiation and number of chemotherapy cycles influencing the time of recurrence after negative second-look operations.

Material And Methods: Having observed complete clinical remission in 356 patients with ovarian cancer, second-look laparotomy was performed. In 180 patients complete pathologic remission was detected and in 73 recurrence was observed.

[Chemotherapy in the treatment of the early relapse of cervical cancer].

Objectives: The treatment with chemotherapy in early recidivations or high clinical stages of the carcinoma of the cervix was analysed.

Materials And Methods: 17 cases early recidivation or high clinical stages in different histopathological types and clinical stages of the cervix cancer were treated with polychemotherapy (5-FU, Adriamycin, Vincrystin and Cyclophosphamide) and monochemotherapy with Carboplatin. The tolerance of these two chemotherapy programs was assessed.

[Intraoperative verification of hysterosalpingographic and laparoscopic examination in cases of oviductal infertility].

There has been made an analysis of 45 cases of women's oviductal infertility qualified to surgical treatment. In search of the reasons of infertility these patients underwent hysterosalpingographic examination and 24 of them additionally, diagnostic laparoscopy with chromotubation. Positive intraoperative verification of HSG and laparoscopy, as a confirmation of tubal obstruction, they obtained at 40 women (88.

[Laparoscopic evaluation of reproductive organs in women treated for infertility].

The authors discuss 84 cases of laparoscopic examination of women with primary or secondary infertility. The patients qualified for this examination had undergone at least 26 weeks of conventional treatment with no effect. In 7 cases the reproductive organ was found to be in order, with fallopian tubes fully patent.

[Laparoscopic verification of hysterosalpingographic image].

The authors discuss 47 cases in which the HSG examination showed secondary tubular unpatency, then verified by laparoscopy. The patients were divided in three groups. The patients who belonged to the first group had two-sides tubular unpatency recognized by HSG.