Publications by authors named "Poonam Katoch"
Article Synopsis
- The study aimed to develop a validated method using RP-HPLC to analyze both the potency and related proteins in human insulin formulations simultaneously.
- The method involves a specific mobile phase and conditions, achieving high sensitivity and accuracy for insulin quantification, with a detection limit of 0.094 mg/ml and accuracy between 99-102.8%.
- By combining the analysis into a single run, the approach aims to save time and costs for quality control in manufacturing and regulatory settings, potentially improving the availability of high-quality insulin products for public health.
In current study, lipase from a thermotolerant TTP-06 was purified in a stepwise manner by using ammonium sulfate precipitation and column chromatography. Thenceforth, it was subjected to sodium dodecyl sulfate- and native-polyacrylamide gel electrophoresis to check the homogeneity of the purified enzyme. The ideal substrate concentration, pH, temperature, reaction duration and lipase specificity were identified.
View Article and Find Full Text PDFBackground: Ubiquitous presence of Mycobacterium fortuitum and ability to cause infections in human beings, hints toward its integral resistance against environmental and host stress conditions. With an aim to identify genes responsible for adapting in vitro acidic stress of M. fortuitum, in the previous study, TnphoA random mutagenesis identified acid susceptible mutant MT727, with mutation in ribosomal maturation factor encoding gene rimP, to be mutated.
View Article and Find Full Text PDFMycobacterium fortuitum has emerged as a nosocomial infectious agent and biofilm formation attributed for the presence of this bacterium in hospital environment. Transposon random mutagenesis was used to identify membrane-proteins for biofilm formation in M. fortuitum.
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