Nanocrystalline cerium dioxide reduces recrystallization in cryopreservation solutions.

Nanocrystalline cerium dioxide is able to protect living cells from oxidative stress under the influence of various stress factors, in particular under the one of low temperatures. This study investigates the phase-structural transformations in aqueous solutions containing CeO nanoparticles (NPs) and their impact on the cryopreservation process. Differential scanning calorimetry and thermomechanical analysis were used to analyse the phase transitions in aqueous suspensions of CeO NPs and aqueous solutions of the cryoprotectant dimethyl sulfoxide (MeSO) with CeO NPs.

[Antibacterial prophylaxis with fosfomycin at the time of the urethral catheter removal after radical prostatectomy (prospective randomized trial)].

Aim: To evaluate the effect of antibacterial prophylaxis using oral fosfomycin during the removal of a urethral catheter after radical prostatectomy on the development of urinary tract infection, severity of leukocyturia and bacteriuria, as well as the severity of lower urinary tract symptoms.

Materials And Methods: A single-center, non-blind, prospective, randomized controlled trial was carried out. The main group included 40 patients, and the control group included 37 patients.

[Functional transcranial dopplerography is a diagnostic tool for cognitive impairment syndrome].

Functional transcranial dopplerography (FTCD) is a non-invasive ultrasound examination that allows recording the dynamics of cerebral blood flow parameters under conditions of factors stimulated the activity of the structures of the central nervous system. Judgments about the sensitivity and specificity of FTCD are based on the close connection between changes in the activity of the nervous (somatic) system and the response of regional cerebral blood flow (CBF). The technique is a portable and accessible diagnostic method used in assessing the possibility of expanding functional activity during the recovery period after a stroke.

Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling.

Microbial rhodopsin (MRs) ion channels and pumps have become invaluable optogenetic tools for neuroscience as well as biomedical applications. Recently, MR-optogenetics expanded towards subcellular organelles opening principally new opportunities in optogenetic control of intracellular metabolism and signaling precise manipulations of organelle ion gradients using light. This new optogenetic field expands the opportunities for basic and medical studies of cancer, cardiovascular, and metabolic disorders, providing more detailed and accurate control of cell physiology.

Culturable Bacterial Endophytes of Wild White Poplar ( L.) Roots: A First Insight into Their Plant Growth-Stimulating and Bioaugmentation Potential.

The white poplar ( L.) has good potential for a green economy and phytoremediation. Bioaugmentation using endophytic bacteria can be considered as a safe strategy to increase poplar productivity and its resistance to toxic urban conditions.

The Molecular Basis for Selectivity of the Cytotoxic Response of Lung Adenocarcinoma Cells to Cold Atmospheric Plasma.

The interaction of cold atmospheric plasma (CAP) with biotargets is accompanied by chemical reactions on their surfaces and insides, and it has great potential as an anticancer approach. This study discovers the molecular mechanisms that may explain the selective death of tumor cells under CAP exposure. To reach this goal, the transcriptional response to CAP treatment was analyzed in A549 lung adenocarcinoma cells and in lung-fibroblast Wi-38 cells.

[Motor impairments in children with autism spectrum disorders: causes and possibilities for correction].

Unlabelled: Autism spectrum disorder (ASD) is characterized by triad of abnormalities in the form of developmental distortion with a lack of communicative abilities, behavioral and motor stereotypies. Etiology and pathogenesis of disease currently are unknown, but multifactorial causes of this pathology have been suggested. Although social disorders are considered a defining characteristic, motor disorders are a key feature of ASD.

Optogenetic cytosol acidification of mammalian cells using an inward proton-pumping rhodopsin.

Ion gradients are a universal form of energy, information storage and conversion in living cells. Advances in optogenetics inspire the development of novel tools towards control of different cellular processes with light. Rhodopsins are perspective tools for optogenetic manipulation of ion gradients in cells and subcellular compartments, controlling pH of the cytosol and intracellular organelles.

[Signaling molecules as biomarkers for predicting implant survival in people of different ages.].

Most patients over 50 years of age are diagnosed with diseases of the dentoalveolar apparatus, in particular, bone tissue disorders, which leads to a decrease in the survival rate of implants. Identification of the causes, as well as the development of a methodology for predicting survival by minimally invasive methods, is relevant. The aim of the study was to study the markers of tight junctions in the buccal epithelium (BE) in people of different ages.

Review on chromatographic and specific detection methodologies for unravelling the complexity of MOAH in foods.

Contamination of foods with mineral oil hydrocarbons, particularly mineral oil aromatic hydrocarbons (MOAH), can potentially pose a health hazard to consumers. However, identifying toxic substances among the many thousands of compounds comprising mineral oils in food samples is a difficult analytical challenge. According to the European Food Safety Authority, there is a lack of concentration and structural data about mineral oil hydrocarbons in foods, and therefore it is not clear to what extent consumers in Europe might be exposed to toxic levels of MOAH.

Fluorescent RET-Based Chemosensor Bearing 1,8-Naphthalimide and Styrylpyridine Chromophores for Ratiometric Detection of Hg and Its Bio-Application.

Dyad compound bearing 1,8-naphthalimide (NI) and styrylpyridine (SP) photoactive units, in which the N-phenylazadithia-15-crown-5 ether receptor is linked with the energy donor naphthalimide chromophore, has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In an aqueous solution, selectively responds to the presence of Hg via the enhancement in the emission intensity of NI due to the inhibition of the photoinduced electron transfer from the receptor to the NI fragment. At the same time, the long wavelength fluorescence band of SP, arising as a result of resonance energy transfer from the excited NI unit, appears to be virtually unchanged upon Hg binding.

Phase transitions and mechanisms of cryoprotection of serum-/xeno-free media based on dextran and dimethyl sulfoxide.

The development of serum-/xeno-free media may help avoid the drawbacks of using serum and its components, such as probable contamination, instability of composition, or difficulty in sterilization. The objectives of this research were to investigate the use of combinations of a permeating cryoprotective agent (MeSO) and non-permeating polymers (polyvinyl alcohol, polyvinylpyrrolidone, polyethylene glycol, hydroxyethyl starch, dextran) for cryopreservation of interstitial cells (ICs) of rat testis, and to propose the mechanism of cryoprotection of such compositions. In the course of this study, the best combination was 100 mg/ml dextran (M.

Strategic aromatic residues in the catalytic cleft of the xyloglucanase MtXgh74 modifying thermostability, mode of enzyme action, and viscosity reduction ability.

The thermostable endo-processive xyloglucanase MtXgh74 from Myceliophthora thermophila was used to study the influence of aromatic amino acids in the catalytic cleft on the mode of action and the ability of enzyme to reduce xyloglucan viscosity. The enzyme derivative Mut I with mutations W64A/W67A in the "negative" subsites of the catalytic cleft resulted in a 5.5-fold increase of the K value.

Crude Extract: Characterization and Analytical Application.

In the present work crude extract (ABE) has been prepared and characterized by its tyrosinase activity, protein composition and substrate specificity. The presence of mushroom tyrosinase (PPO3) in ABE has been confirmed using two-dimensional electrophoresis, followed by MALDI TOF/TOF MS-based analysis. GH27 alpha-glucosidases, GH47 alpha-mannosidases, GH20 hexosaminidases, and alkaline phosphatases have been also detected in ABE.

Dataset on the interview-based survey of Moscow bicycle infrastructure.

The data were collected using Computer Assisted Telephone Interview with a questionnaire set of closed-ended and open-ended questions. Respondents' phone numbers were randomly generated. The survey sample included 1000 adult citizens of Moscow - active and potential users of bicycles (in equal proportions) who take or can take regular rides to destinations up to 5 km away, as of August 2018.

Inclusive growth: A dataset on key and institutional foundations for inclusive development of Russian regions.

This article presents a dataset for calculating the index of inclusive growth in the regions of the Russian Federation, estimated on key and institutional foundations of the performance of inclusive development. The authors of the research used the methodology of the World Economic Bank and the World Economic Forum, based on a comparative analysis of key and institutional indicators of the performance of territorial entities, which they adapted to the socio-economic features of the regional division of Russia. For the purpose of formation of a dataset was executed assessment of the inclusive growth index for 26 regions of the Russian Federation, it allowed to define the strengths and weaknesses of the inclusive development of each region of the Russian Federation.

Regional free cash flow dataset: An approach to regional performance evaluation.

This data article provides estimates on the Russian regions' aggregate free cash flow, which is not covered by national statistics of major countries. A proper microeconomic model was adapted to regional level data to derive a synthetic indicator of a regional economy's performance. The data contributes to the set of regional performance measures thus enabling a new look at studies of economic growth and development.

[Early carotid endarterectomy in patients with symptomatic carotid stenosis in the acute period of ischemic stroke].

Aim: To identify the optimal timing of CEA in patients with hemodynamic significant stenosis of carotid arteries in the acute period of ischemic stroke (IS).

Material And Methods: Five samples (n=30 911) based on the search results from January 2008 to April 2016 in Russian and English-language scientific databases were analyzed. The main endpoints of the studies in both groups (repeated stroke, death, cardiac complications, frequency of restenosis) were studied depending on the timing of operative intervention and preoperative selection criteria.

Structural and Functional Characterization of the BcsG Subunit of the Cellulose Synthase in Salmonella typhimurium.

Many bacteria secrete cellulose, which forms the structural basis for bacterial multicellular aggregates, termed biofilms. The cellulose synthase complex of Salmonella typhimurium consists of the catalytic subunits BcsA and BcsB and several auxiliary subunits that are encoded by two divergently transcribed operons, bcsRQABZC and bcsEFG. Expression of the bcsEFG operon is required for full-scale cellulose production, but the functions of its products are not fully understood.

[The informational technologies for the comprehensive rehabilitation of the patients presenting with juvenile cerebral palsy (a review)].

This article is devoted to the application of the up-to-date technologies for the treatment and rehabilitation of the children presenting with juvenile cerebral palsy (JCP). It is shown that the use of computer classes, play therapy toys, and robotic exercise machines collectively make up a promising tool for the correction of limb movements in the JCP patients. Lessons with the use of modified technical devices make it possible to develop the creative capacities of the children, improve memory, attention, coordination and precision of the movements of their extremities.

The development of the cell cryopreservation protocol with controlled rate thawing.

Thawing in the water bath is often considered as a standard procedure. The thermal history of samples thawed in this way is poorly controlled, but cryopreservation and banking of cell-based products require standardization, automation and safety of all the technological stages including thawing. The programmable freezers allow implementation of the controlled cooling as well as the controlled thawing.

Proteogenomics meets cancer immunology: mass spectrometric discovery and analysis of neoantigens.

Cancer proteogenomics is an emerging field that aims to identify and quantify protein sequence changes associated with the cancer genome. Besides being involved in cancer development and progression, such protein variants may serve as neoantigens, which provide the T-cell response against tumors. Mass spectrometry-based proteogenomics may be a promising tool for finding neoantigens in individual specimens.