A search for genetic markers of an endophytic ecotype of Bacillus amyloliquefaciens was performed by the subtractive hybridization technique. The isolated fragments specificity was checked by DNA-DNA hybridization. The sequence of a DNA locus exhibiting the highest taxon specificity has manifested a 76% identity to the gene mtlA from Bacillus subtilis 168 genome.
View Article and Find Full Text PDFDetermined by means of thermoluminescent dosimetry radiation exposure to a patient from punctures controlled by two-projection x-ray and TV was, on an average, 0.0176 Gy +/- 18%, and when controlled by CT it was 0.048 Gy +/- 22% which was comparable with dose exposure of 2 x-ray films in the first case and of 5 x-ray films in the second case.
View Article and Find Full Text PDFThe results of measurement of labelling index in tumor tissues and the so-called "normal" gastric mucosa, used as a cell kinetics parameter, were compared in 21 cases of stomach cancer. A procedure of double labeling DNA-synthesizing cells with different concentrations of 3H-thymidine was applied. Tumor tissue samples and specimens of gastric mucosa obtained at a distance of 5-10 cm from the visible boundary of tumor were labeled simultaneously, and a significant difference in labeling index in the tissue samples was observed.
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