Rational Formulation of targeted ABT-737 nanoparticles by self-assembled polypeptides and designed peptides.

Here we present the development of nanoparticles (NPs) formulations specifically designed for targeting the antiapoptotic Bcl-2 proteins on the outer membrane of mitochondria with the drug agent ABT-737. The NPs which are self-assembled by the natural polypeptide poly gamma glutamic acid (ϒPGA) and a designed cationic and amphiphilic peptide (PFK) have been shown to target drugs toward mitochondria. In this study we systematically developed the formulation of such NPs loaded with the ABT-737 and demonstrated the cytotoxic effect of the best identified formulation on MDA-MB-231 cells.

Staphylococcus aureus functional amyloids catalyze degradation of β-lactam antibiotics.

Antibiotic resistance of bacteria is considered one of the most alarming developments in modern medicine. While varied pathways for bacteria acquiring antibiotic resistance have been identified, there still are open questions concerning the mechanisms underlying resistance. Here, we show that alpha phenol-soluble modulins (PSMαs), functional bacterial amyloids secreted by Staphylococcus aureus, catalyze hydrolysis of β-lactams, a prominent class of antibiotic compounds.

Hybrid Hydrogels of FKF-Peptide Assemblies and Gelatin for Sustained Antimicrobial Activity.

The growing resistance of pathogenic bacteria to conventional antibiotics promotes the development of new antimicrobial agents, including peptides. Hydrogels composed of antimicrobial peptides (AMPs) may be applied as topical treatments for skin infection and wound regeneration. The unique antimicrobial and ultrashort-peptide FKF (Phe-Lys-Phe) was recently demonstrated to form bactericidal hydrogels.

Assembly of cationic and amphiphilic β-sheet FKF tripeptide confers antibacterial activity.

The race drawn against bacteria facing the evolution of antimicrobial resistance fuels research for new drugs and therapeutic strategies. FKF, a tripeptide that is cationic and amphiphilic was examined in light of its potential antimicrobial activity. Acid titration of purified peptide solution, 6% w/v (136 mM), yielded a hydrogel at pH~ 4.