[Evaluation of selected parameters of lymphocyte activity in umbilical blood. II. Release of angiogenic cytokines].

Angiogenic abilities of mononuclear cells in the umbilical blood was estimated in 31 healthy newborns. LIA test was performed on 6-8 weeks old mice, which underwent immunosuppression before intracutaneous injection of leucocytes and were narcotized after 72 hours. The mean number of newly formed vessels was 12.

A ribonuclease inhibitor expresses anti-angiogenic properties and leads to reduced tumor growth in mice.

Our experiments were designed to determine whether recombinant ribonuclease inhibitor (RNasin) could inhibit angiogenesis and reduce tumor growth in adult mice. We used the Fajardo disc angiogenesis assay as the primary means of measuring new blood vessel growth. This assay measures the penetration of cells into a polyvinyl alcohol sponge with a central core of ELVAX-coated sponge containing test substances.

Assays for angiogenesis: a review.

Accurate, reliable quantitation of the neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new blood vessel growth. Over many years, ingenious ways have been developed for measuring this process, and they have contributed much to our present understanding of the vasculogenesis and angiogenesis that accompany normal embryonic development, lactation and wound healing, as well as tumor growth and a variety of other disease states ranging from diabetic retinopathy to autoimmune vasculitis. In this review we describe and evaluate the methodology and specific features of some of the most frequently used of these assays.

[Studies on impaired angiogenesis activity of the leucocytes in patients with oral candidiasis].

The preliminary characteristics is reported of the cells causing active suppression leading to a decrease of the angiogenesis activity of leucocytes isolated from patients with oral candidiasis. The effect of three immunomodulators on this suppression was studied.

[Subpopulations of t lymphocytes in patients with ischemic heart disease].

T-cells subpopulations were determined with theophylline test in 42 patients with the ischemic heart disease, including 31 patients with the history of myocardial infarction, and in 78 healthy volunteers. The patients' age was between 20 and 40 years and between 60 and 80 years. Defect of theophylline-sensitive fraction (suppressor T-cells and cytotoxically not specific lymphocytes in young patients has been observed.

Effect of endurance and "sprint" physical training on lymphocyte-induced angiogenesis in rats.

Angiogenic activity of mononuclear leucocytes (MNC) obtained from the spleen was studied in rats subjected to 5-week endurance or "sprint" exercise training. Increased angiogenic activity of MNC, accompanied by an elevation of FeG+ lymphocyte number, was found only in animals performing very intensive, short-term efforts ("sprint" training).

A contribution to studies on the mechanism of lymphocyte-induced angiogenesis: human peripheral blood mononuclear leucocytes with highest angiogenic activity.

Using the lymphocyte-induced angiogenesis test a comparison of angiogenic activity of some mononuclear cell fractions was performed. It is shown that the highest angiogenic activity was expressed by cells bearing both the receptor for the Fe portion of IgG and a CD4 surface antigen.

Modulatory effect of sera from scleroderma patients on lymphocyte-induced angiogenesis. II. Effector cells for the enhancing effect of acroscleroderma patients' sera.

In our previous study, using the modified lymphocyte-induced angiogenesis assay, we found that incubation of normal human peripheral blood mononuclear cells (PBMCs) with sera from patients with acroscleroderma, but not with diffuse scleroderma, markedly enhanced their angiogenic capability. The results of the present work suggest that this enhancement is mediated by lymphocytes bearing receptors for the Fc portion of IgG and belonging mainly to the CD4+ T-cell subset.

Theophylline-resistant and theophylline-sensitive "early" and "late" E rosette-forming cells in young and aged humans.

The percentage of cells with high-affinity sheep red blood cell (SRBC) receptors, "active" or "early" rosette-forming cells, (ARFC) in theophylline-resistant peripheral blood T lymphocytes achieve higher values in aged subjects and, in particular, in the group with clinically manifested atherosclerosis. A diminished OKT-4/OKT-8 ratio in theophylline-resistant ARFC was noticed in this group. On the contrary, the proportion of the theophylline-resistant cells with low-affinity receptors, cold or late rosette-forming cells (CRFC) attained lower values in aged subjects than in young subjects.

Serum samples from patients with active psoriasis enhance lymphocyte-induced angiogenesis and modulate endothelial cell proliferation.

In former studies we have shown the increased angiogenic capability of peripheral blood mononuclear cells (MNCs) from patients with the active guttate type of psoriasis vulgaris. The present study tested the effects of serum factors from 67 patients with various forms of psoriasis vulgaris and from 32 healthy individuals on the angiogenic capability of normal human MNCs in an animal system. The angiogenesis-enhancing effect was most marked in the serum samples from patients with plaquelike and peripherally spreading lesions lasting for one to two months, and tended to disappear in patients with long-lasting and/or stationary lesions.

Altered immune reactivity of mice born from mothers treated with ampicillin during gestation.

The cellular and humoral immune responses of 30-day-old mice born from mothers treated with ampicillin during pregnancy were studied. A lowering of cellular immunity, accompanied by a decrease in T lymphocyte percentage in lymph nodes, and an increase of humoral immune response were observed.

Enhanced angiogenic capability of monocyte-enriched mononuclear cell suspensions from patients with systemic scleroderma.

Different subsets of peripheral blood mononuclear cells (MNC) from 15 patients with systemic scleroderma were tested for their ability to evoke angiogenesis in a xenogenic system. The angiogenic capability of total MNC from patients with systemic scleroderma was lower than that of normal human cells, irrespective of the form of the disease. However, the capability of a monocyte-enriched subset of MNC from patients with scleroderma was found to be increased, as compared with their total MNC and with that of the corresponding subset from healthy individuals.

Modulatory effect of sera from scleroderma patients on lymphocyte-induced angiogenesis.

Sera from 22 patients with progressive systemic sclerosis were tested for the ability to modify the angiogenic capability of normal human mononuclear cells. The sera from patients with acrosclerosis, including the abortive form (CREST syndrome: calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias), markedly enhanced this capability compared with sera from both healthy donors and patients with severe acrosclerosis and diffuse scleroderma. The enhancing effect of sera from patients with acrosclerosis decreased and/or disappeared in cases where the patient's acrosclerosis was chronic and severe.

The effect of experimental hyperlipidemia on the angiogenic activity of rabbit peripheral blood lymphocytes.

Peripheral blood lymphocytes from young rabbits with experimentally evoked hyperlipidemia and atherosclerosis express enhanced angiogeneic activity. It seems that angiogenesis-enhancing factor in the sera of cholesterol fed rabbits is not present.