Multiparameter flow cytometry for simultaneous assessment of p53 protein expression and cellular DNA content in oral squamous cell carcinomas: evidence for the development of aneuploid clones from p53-deficient diploid progenitor cells.

Diploid tumour cells regularly continue to progress after the development of aneuploid cell populations in head and neck squamous cell carcinomas. The coexistence of aneuploid clones with their diploid progenitor cells provides a unique opportunity to study the order of appearance of p53 mutation and aneuploidy in the same tumour. Multiparameter flow cytometry was therefore applied to 22 oral squamous cell carcinomas to simultaneously assess cellular DNA content and p53 protein expression on a single-cell basis.

Progression of diploid tumor cells in aneuploid head and neck squamous cell carcinomas.

The development of aneuploid clones from diploid progenitor cells is a regular characteristic of head and neck squamous cell carcinoma progression. While the significance of aneuploidy formation for the acquisition of invasive and metastatic behavior is well documented, little is known about the contribution of diploid tumor cells after aneuploid clones have emerged. To distinguish diploid cells of epithelial origin from benign cellular components, we applied multiparameter flow cytometry of DNA content and cytokeratin (CK) expression to 36 primary tumors.

High-resolution DNA flow cytometry in papillary cystadenoma lymphomatosum (Warthin's tumour).

Twenty-eight examples of papillary cystadenoma lymphomatosum (Warthin's tumour) of the parotid gland were analysed by high-resolution DNA flow cytometry. The mean coefficient of variation was found to be 1.19% (SD: 0.

Representativity of incisional biopsies for the assessment of flow cytometric DNA content in head and neck squamous cell carcinoma.

DNA flow cytometry studies of squamous cell carcinoma of the head and neck have shown that patients with diploid tumours have favourable prognoses, whereas the outcomes of those with DNA aneuploid tumours are poor. DNA flow cytometry is therefore increasingly used as an integral part of diagnostic procedures. To evaluate how representative biopsies predict the DNA ploidy of oral carcinomas, incisional biopsies taken pretherapeutically from 256 tumours were compared with the corresponding surgical resection specimens.

[Potentiation of the effects of anxiolytics with magnesium salts].

In a double blind trial the authors tested the assumption that magnesium potentiates by its specific action the therapeutic effect of anxiolytics. A group of 20 women treated on account of anxious depressive neurosis was divided into two sub-groups. The first was given anxiolytic and placebo, the second one anxiolytics and Mg-lactate in corresponding amounts.

Health problems of windowless henhouses.

The working, health and zoohygiene problems of keeping chickens (45,000) in TEROZ type windowless houses on deep litter with controlled light regimen and hens (75,000) in cages were the subject of study for 3 years. The poultry was fed on factory-produced feedstuffs with the then permitted addition of CTC in amounts of 20 g/l tonne. Except for the low intensity of illumination in the chicken house (10-1.