[Hypercoagulation syndrome in multiple myeloma].

Aim: To determine the frequency and pattern of blood clotting disorders in patients with multiple myeloma (MM) and to evaluate the adequacy of preventive anticoagulant or antiaggregant therapy.

Subjects And Methods: The prospective study conducted at the Department for High-Dose Chemotherapy and Bone Marrow Transplantation, Hematology Research Center, Ministry of Health of the Russian Federation, in March 2012 to May 2013, enrolled 25 patients (13 men and 12 women) aged 29-72 years (median age 54 years) with new-onset MM. The latter was staged using the Durie-Salmon classification: Stages I, II, and III were determined in 2, 10, and 13 patients, respectively.

[Effect of bortezomib on the efficiency of hematopoietic stem cell mobilization in patients with multiple myeloma].

Aim: To study the results of mobilizing and collecting autologous hematopoietic stem cells (HSC) in patients with multiple myeloma (MM) receiving bortezomib as part of induction therapy regimens.

Materials And Methods: In June 2001 to April 2010, the Department of Bone Marrow Transplantation, Hematology Research Center, Ministry of Health and Social Development of Russia, mobilized autologous HSC in 93 patients with MM, by using cyclophosphan (CF) and granulocyte colony-stimulating factor. The analysis covered 73 patients who received VAD and/or bortezomib-containing courses as induction therapy.

[Optimization of mobilization regimes of blood hemopoietic stem cells in patients with multiple myeloma].

Aim: To determine an optimal cyclophosphamide dose in the mobilization scheme providing adequate collection of CD34+ cells in patients with multiple myeloma (MM), to optimize the time of initiation of granulocytic colony-stimulating factor (G-CSF) administration, to study effects of induction therapy schemes on results of mobilization and collection of CD34+ cells.

Material And Methods: Department of hemoblastoses chemotherapy and bone marrow transplantation of the Russian Hematological Center performed mobilization of autologous blood hemopoietic stem cells (BHSC) in 93 MM patients treated in 2001-2010. This was done with cyclophosphamide and G-CSF.

[Prolonged bone marrow aplasia in patients with acute leukemia after chemotherapy].

Aim: To analyze the causes of prolonged hematopoietic tissue aplasias in patients with acute leukemias (AL) after chemotherapy courses.

Materials And Methods: Data on 7 patients with acute myeloid leukemia, followed up at the Hematology Departments, Hematology Research Center, Russian Academy of Medical Sciences, over the period 2003 to 2007, who had developed deep bone marrow aplasia (BMA) inadequate to cytostatic drug exposure during chemotherapy, were analyzed. The authors compared in all the patients the values of peripheral blood and bone marrow (BM) puncture specimens and the results of blood tests using the polymerase chain reaction at different AL development stages with the results of an immunohistochemical study using the markers of viruses of hepatitis C and B, a herpes group (EBV, CMV, HSV-1, HSV-2) and parvovirus B19.

[Transplantations of allogenic and autologous hemopoietic stem cells in acute leukemia (results of 20-year experience)].

Aim: To analyse results of transplantation of allogenic and autologous hemopoietic stem cells (allo-THSC and auto-THSC) with myeloablation preconditioning in patients with acute leukemia (AL) performed in 1987-2006.

Material And Methods: A total of 71 allogenic and 45 autologous THSC were performed in 116 patients with different AL variants. Conditioning in all allo-THSC included busulfan (16 mg/kg) and cyclophosphamide (120 mg/kg).

[Infectious complications after transplantation of autologous hemopoietic blood cells in patients with hematological malignancies].

Aim: To characterize infectious complications arising within 30 days after transplantation of autologous hemopoietic blood cells in 42 patients with hematological malignancy (HM); to compare the course of early posttransplantation period with reference to a kind of high-dose conditioning and dose of transplanted CD34+ cells.

Material And Methods: Autotransplantation (AT) was conducted as consolidation of a complete or partial remission in 20 patients with multiple myeloma, 14 patients with lymphogranulomatosis and lymphosarcoma, 7 patients with acute leukemia and 1 patient with rabdomyosarcoma. The program of pretransplantation conditioning corresponded to the disease form and included: melphalan, BEAM, busulphane-cyclophosphamide.

[Is leukemic transformation of donor cells possible?].

Aim: To genotype tumor cells in the recurrence of leukemia after allogenic transplantation of bone marrow (TBM).

Material And Methods: Standard cytogenetics and fluorescent hybridization in situ (FISH) with a probe to the centrometic sites of X/Y chromosomes were used in examination of 2 patients with acute promyelocytic and acute non-differentiated leukemia after allogenic TBM from donors of the opposite gender. Bone marrow was studied 1, 2, 3, 6, 9, 12, 15, 17, 18 months after the transplantation.

[A study of the effect of the adsorbed molecules of concanavalin A on characteristics of the bound water in the hydrate coat of the disperse silica by 1H NMR of the frozen water suspensions].

The experimental technique is presented which allows the investigation of water at the adsorbent-biopolymer interface by record of temperature dependencies of 1H NMR signals during the heating of previously frozen water suspensions. It was found that the adsorption of protein conconovalin A resulted in twofold decrease of strong bound water amount and in 70% increase of loosely bound one. The mean-value of Gibbs energy decreased twice for strong bound water and increased twice for loosely bound one.

[Cloning and partial characteristics of the barley D-hordein sequence].

A number of clones containing major endosperm-specifically transcribed gene copies were selected from a cDNA library developed on the basis of barley endosperm mRNA. Approx. 30% of the recombinant clones carried sequences homologous to mRNA of various cereal storage proteins.

[Interaction of Candida albicans with macrophages].

Study of the in vitro interaction of mouse peritoneal macrophages with C. albicans has revealed that these macrophages, though easily phagocytizing C. albicans blastospores, are incapable of destroying the fungus.

[Immune system function in candidiasis patients].

To compare the clinical picture and the immunological characteristics, 58 candidiasis patients differing by the severity and dissemination of the disease were examined. Chronic candidiasis of the skin and mucous membranes, the most severe and disseminated form of the disease, is associated with a decrease in the number of T-lymphocytes and changes in their subpopulations, as well as high titers of Candida albicans antigen and antibodies to it in blood sera. The immune system of patients with visceral candidiasis and chronic vulvovaginal candidiasis was similar to that of healthy persons in the characteristics under study.

[Nature of the restructuring of the T- and B-immunity systems in patients with cutaneous and visceral candidiasis].

The examination of 90 patients with superficial and visceral candidiasis, as well as Candida carriers, has revealed that the heavy contamination of the body with fungi of the genus Candida is accompanied by the specific transformation of the immune system, manifested by differences in the character of sensitization to Candida antigens: in the patients with superficial and visceral candidiasis the B-system of immunity is sensitized to a greater degree, while in the Candida carriers the sensitization of the T-system is more pronounced. Despite the fact that the immune system of patients with the disseminated form of visceral candidiasis is capable of developing the delayed hypersensitivity (DH) reaction to bacterial allergens, such patients have been shown to develop DH to Candida antigens only in rare cases. This is probably due to disturbances in their immunocompetent systems, caused, seemingly, by the secondary immune insufficiency.

[T- and B-immunity system indices in candidiasis].

The examination of 73 patients with superficial and visceral candidiasis and Candida-carriers yielded no data to the effect that the increased activity of opportunistic fungi of the genus Candida in the body was preceded by organic lesions in the T- and B-systems of lymphocytes. Only in 16 patients who had visceral candidiasis for 8-12 years with combined lesions of several organs the decreased capacity of lymphocytes for reacting with phytohemagglutinin was observed, which was probably a manifestation of the secondary immune insufficiency, developing in the presence of prolonged infection.