Magnetic resonance imaging of canine mast cell tumors.

Mast cell tumors (MCT) are the most common cutaneous tumors in dogs. Our purpose was to describe the magnetic resonance (MR) imaging characteristics of cutaneous MCT and to identify imaging characteristics that allow differentiation of metastatic from normal lymph nodes. Eight dogs with a total of nineMCT were imaged as were their presumed draining and associated contralateral lymph nodes.

Is laser assisted angioplasty a real alternative to surgical treatment of occluded peripheral vessels?

Since 1988 250 patients had occluded peripheral arteries reopened by an Argon laser. The mean age of the 110 men and 85 women was 71 years and the mean length of all occlusions 13.4 cm.

Comparative studies on the polyamine metabolism and DFMO treatment of MCF-7 and MDA-MB-231 breast cancer cell lines and xenografts.

In order to characterize the estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 human breast cancer cells and xenografts, their growth kinetic parameters and some biochemical characteristics concerning the receptor status and polyamine metabolism were determined and compared. The doubling times calculated from the growth curves showed higher proliferation rate of MDA-MB-231 cells, both in culture (21 hours) and in xenograft (9.7 days), in comparison to the MCF-7 cells which had values of 32 hours and 11.

[Quantitative flow measurement of the art. dorsalis pedis in normal probands and patients with arterial occlusive disease of a low Fontaine stage].

Quantitative blood flow measurements of the dorsal foot artery were performed with a multichannel digital pulsed Doppler device in normal volunteers and in patients with Fontaine IIa stage peripheral arterial occlusive disease. The measured parameters were: 1. vessel diameter, 2.

[Initial experiences with transpopliteal, laser-assisted balloon angioplasty of femoral artery occlusions].

For many years great efforts are being made to recanalise totally occluded vessels and averting a bypass operation as long as possible. In two years 190 patients were treated successfully by the Lastac System. Encouraged by very good results, we started to recanalise the totally occluded superficial femoral artery via a transpopliteal approach.

[Recanalization of peripheral vascular occlusions using an argon laser].

A new Argonlaser developed in the United States of America is available to the radiological and surgical departments of the Hospital of St. Pölten. It is used for recanalization of totally occluded peripheral arteries.

Changes in the polyamine and nucleotide metabolism of P388 leukemia cells treated with DL-alpha-difluoromethylornithine in culture.

Influence of DL-alpha-difluoromethylornithine (DFMO) treatment on the growth kinetics, labelling index, extra- and intracellular polyamine and nucleotide concentrations was monitored in cultured P388 leukemia cells. A substantial decrease of cell proliferation was observed when the cells were continuously treated with 1-5 mM DFMO. Depletion of cellular polyamines, mostly of putrescine and spermidine, was seen with a concomitant but delayed increase of spermidine and spermine levels in the culture medium.

Permanent metachromatic effect of 1,9-dimethyl-methylene blue on acidic mucopolysaccharides of mastocytes.

The 1,9-dimethyl-methylene blue (SERVA) has a very strong metachromatic reaction with sulphated mucopolysaccharides of mastocytes at pH = 1 (Tris-HCl buffer). This metachromasia is retained also at increasing pH (2.5-5), and is not affected either by the quality or by the concentration of the ions present (1/240 M Tris-HCl, and 0.

Cytochemical characterization of Yoshida sarcoma cells resistant to dibromodulcitol.

Two Yoshida ascites sarcoma cell populations, one of them originally sensitive and another rendered resistant to the alkylating agent dibromodulcitol (DBD), were compared for doubling time, labeling index, survival time, morphological features, cellular DNA content and modal DNA value. Cross-resistance studies were carried out on bilaterally growing solid tumours derived from the sensitive and resistant Yoshida cell populations. Glycogen-containing granules appeared in the cytoplasm of the DBD-resistant sarcoma cells; these were not present in the sensitive tumour.

The effect of N-formylleurosine on DNA synthesis of Ehrlich ascites tumor.

Some of the vinca alkaloids are well known and widely used in clinical practice, in spite of their numerous side-effects. For the elimination of untoward side-effects semi-synthetic alkaloids have been produced. This work reports on N-formylleurosine (N-F-Leu), one of these agents.

Study of the antitumoral activity of S-carbamoyl-L-cysteine derivatives in animal experiments.

The antitumoral activity of three amino acid derivatives, S-carbamoyl-L-cysteine, S-ethyl-carbamoyl-L-cysteine, and S-chloroethyl-carbamoyl-L-cysteine, was studied. The ethyl and chloroethyl derivatives had a pronounced curative effect on certain animal tumors. It is interesting in the case of S-carbamoyl-L-cysteine that N-ethylization of the substance resulted in increased chemotherapeutic effectivity with no simultaneous change of the toxicity.

Cytophotometric determination of basic proteins with heparin-alcian blue method.

For the quantitative cytochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelectrophoretic examinations) and no aspecific staining (Millipore filter model). In EHRLICH ascites tumour cells, changes in the FEULGEN-DNA content is followed by the proportional changes of HAB-basic protein content.