DISTRIBUTION AND FREQUENCY OF BLASTOCYSTIS SP. BY METHODS OF MICROSCOPY AND CULTIVATION IN FAECES OF RESIDENTS OF KHARKOV REGION.

Blastocystis sp. - is the most prevalent anaerobic intestinal protozoan parasite in humans and many animals; from 1 to 2 billion people in the world are colonized by this pathogen. Blastocystis sp.

Seroprevalence of babesiosis in immunocompetent and immunocompromised individuals.

Unlabelled: Interest in Babesia species is gaining an increasing attention as an emerging tick-borne pathogen. Infection is primarily transmitted through Ixodes ticks, and alternatively by blood transfusions from asymptomatic donors.

Aim: The aim of the study was detection of Babesia seroprevalence in different groups of population with the usage of experimental B.

Implementation and analysis of Babesia immunoassay testing.

Unlabelled: Lifelong withdrawal from the donor population of those who have been diagnosed with babesiosis must be used for transmission prevention.

Aim: The aim of the study was a detection of Babesia antibodies level with the usage of experimental Babesia divergens whole-cell slide antigen and commercial B. microti immunofluorescence assay substrate slide (Fuller Laboratories, USA).

Anaplasmosis: experimental immunodeficient state model.

Objective: Introduction: The recently described anaplasmosis infection is widespread but concerns to the insufficiently known group of diseases. The aim of our research is the development of uniform biological model for reproducing of artificial immunodeficient state by experimental anaplasmosis.

Patients And Methods: Materials and methods: Algorithm of experimental anaplasmosis reproducing, consisted of such consecutive stages: 1) artificial forming of the immunodeficient state at nonlinear white mise (Mus musculus L.

Use of Piezoelectric Immunosensors for Detection of Interferon-Gamma Interaction with Specific Antibodies in the Presence of Released-Active Forms of Antibodies to Interferon-Gamma.

In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected.

[Characterization of the lipopolysaccharide from Budvicia aquatica DLR 20186].

A lipopolysaccharide (LPS) from Budvicia aquatica DRL 20186 was isolated, studied, and chemically identified. It was shown to be lowly toxic, but highly pyrogenic. Its fatty acid composition was similar to that of the LPS from other Enterobacteriaceae, with predominance of tetradecanoic (32.

[Biological activity of Budvicia aquatica lipopolysaccharides].

The fatty acid composition of lipopolysaccharides (LPS) lipids A of Budvicia aquatica strains (n = 6)--representatives of Enterobacteriaceae new species are studied for the first time. It was established that fatty acids with the length of carbon chains from C12 to C18 are presented. All of B.

[Characterization of Budvicia aquatica lipopolysaccharides].

For the first time lipopolysaccharides (LPS) of 6 Budvicia aquatica strains--representatives of new Enterobacteriaceae species have been isolated. It was shown that the yield of LPS ranged from 0.9 to 7.

[Characteristics of structure components of Rahnella aquatilis lipopolysaccharides].

LPS have been isolated from 6 strains of Rahnella aquatilis, their separate structure components have been obtained: lipid A, O-specific polysaccharide shain (OPS) and core oligosaccharide. 3-Oxytetradecanoic (31.7%) and tetradecanoic (17.

[The results of a 5-year study of listeriosis in Ukraine].

Data on the isolation of Listeria spp. cultures on the territory of Ukraine, the frequency of Listeria contamination of alimentary raw materials and foodstuffs, the level of listeriosis morbidity, the clinical forms of the manifestation of this disease, as well as the occurrence of asymptomatic carriership among clinically healthy persons of epidemiologically significant professions, are given. The results of the study of the biological properties of 73 strains of Listeria spp.

[Listeria bacterial carriage: a method for sanative treatment].

1.6% of clinically healthy women active in certain professions have been found to be carriers of pathogenic species of Listeria. The study of the sensitivity of 73 strains of Listeria spp.

[Development and approbation of polymerase chain reaction for detection of pathogen in Listeria infection].

A system of primers for detection of Listeria by polymerase chain reaction (PCR) has been developed. Specificity and sensitivity of the method was evaluated by analyses of reference clinical and abiotic samples. Clinical trials of PCR were carried by detecting latent (asymptomatic) carriers of Listeria among healthy women of epidemiologically significant professions.

[Development and testing of polymerase chain reaction method for detection of meningococcal infection pathogen].

A system of primers has been developed for the diagnosis of meningococcal infection has been developed on the basis of the polymerase chain reaction (PCR). Specificity and sensitivity of detection of N. meningitidis has been evaluated in analysis of the model clinical specimens.

[The pathogenicity enzymes of clinical strains of Klebsiella pneumoniae].

In 108 K. pneumoniae clinical strains isolated in pneumonia (32 strains), inflammatory processes of the urinary tracts (36 strains) and toxicoseptic states (40 strains) caseinolytic, gelatinase, phosphatase, lecithinase activities and the capacity for producing DNAase and RNAase were studied. The presence of caseinolytic activity was found in 38 cultures (35.

[The determination of the volume (weight) of an inoculate applied using the Semenikhina-designed replicator].

The weight (volume) of inoculate applied with Semenikhina's replicator stamp with 1, 2, and 3-mm needles is determined. The method is sufficiently precise for microbiological screenings. The volume of inoculate depends on the diameter and geometrical shape of the needle and characteristics of inoculated substance and surface onto which it is applied.

[The biological properties of adsorbed and nonadsorbed OH-agglutinating sera for the identification of Rahnella aquatilis bacteria].

The paper presents the production technology of rabbit hyperimmune adsorbed and unadsorbed OH-agglutinating sera for Rahnella aquatilis bacteria identification. Results are reported for such biological characteristics of the new preparations as their specificity and agglutination activity (titer). It is shown that cultures R.

[Rarely encountered, atypical and new species of enterobacteria].

The article characterizes "rare", atypical and new individualized representatives of Enterobacteriaceae family. Classification approach is formulated which makes it possible to subdivide enterobacteria into three groups according to frequency of isolation, genetic determination and degree of atypicality. Schemes are presented for differential diagnosis for correct carrying out laboratory investigations and adequate interpretation of bacteriological assays results.

[Species of enterobacteria new to medicine].

The paper presents main biological characteristics of six new species of enterobacteria, which may cause a number of infectious processes in man: Moellerella wisconsensis, Koserella trabulsii. Leclercia adecarboxylata. Escherichia fergusonii.

[The characteristics of the biological properties of Listeria strains isolated in Ukraine and Belgium].

Biological characteristics of 21 strains of bacteria of Listeria genus have been studied in Ukraine and Belgium. Morphological, cultural biochemical properties of cultures have been described. It has been proved that 85.

[A method of preparing specimens for the electron microscopic study of bacterial fimbrial structures].

A method for making bacterial preparations for studies of fimbrial structures by electron microscopy has been developed, making use of impressions of 'young' colonies in agar microscopy. The suggested method involves three steps: cultivation of the examined bacteria in lamellar media till they attain the 'young' age; agar microscopy of the grown culture and making of impressions from the selected sites; contrasting of the objects and drying of the preparations. The new method is simple, economic, highly reliable and yields good results.

[An evaluation of the biochemical typing of Klebsiella cultures].

The paper deals with the results of analysis of 219 strains of Klebsiella using 12 tests--fermentation of adonite, asparaginic acid, sodium citrate, dulcite, d-tartrate, glutamic acid, inosite, L-proline, sodium malonate; reactions with methyl red, Foges-Proscauer, with 5-ASA. A scheme for determining fermentovars of Klebsiella is suggested which includes the tests--fermentation of adonite, dulcite, d-tartrate, glutamic acid; color reaction with 5-ASA. The groups of Klebsiella different in origin are characterized by nonhomogeneous distribution of different fermentovars.

[The bacteriocin typing of Klebsiella pneumoniae strains].

Results of bacteriocin typing of 196 strains of the Klebsiella genus are presented. They are typified by their sensitivity to bacteriocins and by their production using colicinogenic and indicating strains from collection of P. Fredericq [correction of Frederick], D.