Matrix-Applied Characterized Autologous Cultured Chondrocytes Versus Microfracture: Two-Year Follow-up of a Prospective Randomized Trial.

Background: Randomized controlled trials studying the efficacy and safety of matrix-applied characterized autologous cultured chondrocytes (MACI) versus microfracture (MFX) for treating cartilage defects are limited.

Purpose: To compare the clinical efficacy and safety of MACI versus MFX in the treatment of patients with symptomatic cartilage defects of the knee.

Study Design: Randomized controlled clinical trial; Level of evidence, 1.

Comparison of the cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation in humans.

Our study compares the histological and immunohistochemical cellular composition of two different chondrocyte-seeded biomaterials and the results of their transplantation. Our study cohort included 21 patients, comprising 19 men and two women with a mean age of 32 years, who were affected by single chondral lesions of the femoral condyles. These patients were enrolled in our study and treated with arthroscopic implantation of the tissue Hyalograft C and/or Brno culture.

Cruciate ligaments in proximal femoral focal deficiency: arthroscopic assessment.

Background: Because there is limited information concerning the cruciate ligaments in proximal femoral focal deficiency, knee arthroscopy was used to identify the changes of cruciate ligaments and their relation to the different types of this deficiency.

Methods: Knee arthroscopy was performed in 21 consecutive patients with deficiency types III, IV, VII-IX using the Pappas classification. A new classification of the knee was created.

[Ipsilateral traumatic dislocation of hip and knee joints. Case report].

Traumatic obturator dislocation of the hip without fracture associated with antero-lateral knee dislocation is considered to be an extreme injury. Such a case, which has never been treated in our department, is reported here. The patient was a 40-year-old man who suffered his injury in a motorcycle accident.

[Total hip replacement from a MIS-AL approach (comparison with a standard anterolateral approach)].

Purpose Of The Study: Minimally invasive surgery (MIS) techniques have recently become a powerful and effective marketing instruments that are often perceived by the patient as the criterion of the surgeon's and institution's standard. In addition to studies reporting the benefits of minimally invasive procedures, some authors have recently found no such benefits or even pointed out some disadvantages. In this paper we present our own view of this issue.

Distribution of chondrocytes containing alpha-smooth muscle actin in human normal, osteoarthrotic, and transplanted articular cartilage.

The aim of our study was to evaluate the occurrence of chondrocytes containing alpha-smooth muscle actin in human normal and diseased cartilage. Immunohistochemistry using monoclonal antibodies for alpha-smooth actin, muscle-specific actin, S-100 protein, CD 34, and desmin was performed on samples of human articular cartilage obtained at autopsy following sudden death, during total hip and knee replacement for osteoarthritis, or after femoral neck fracture in patients without symptoms of osteoarthritis. Moreover, the layers of residual cartilage from chondral posttraumatic defects obtained during preoperative arthroscopy and of newly formed cartilage after autologous-chondrocyte transplantation (Hyalograft C) obtained during second-look arthroscopy were also examined by immunohistochemistry and RT PCR.

[Treatment of deep cartilage defects of the knee with autologous chondrocyte transplantation on a hyaluronic Acid ester scaffolds (Hyalograft C)].

Purpose Of The Study: The treatment of chondral defects by transplantation of autologous chondrocytes has recently shown further development. Various biomaterials are used as carriers facilitating attachment and even distribution of chondrocytes in the defect. Since 2003 Hyalograft C, hyaluronan-based scaffolds, has been used, in a clinical study, for implantation of autologous chondrocytes in the treatment of deep chondral lesions of the knee at our department.

Desmin-positive and alpha-smooth muscle actin positive chondrocytes in human defective articular cartilage--preliminary report.

We studied the results of immunostaining for S-100 protein, alpha-smooth muscle actin, muscle specific actin and desmin in articular cartilage specimens obtained during an arthroscopy from eight patients with different degrees of osteoarthritis of the knee joint. In all cases, most of the cartilage cells were strongly positive for S-100 protein. Actin positive chondrocytes were present in four samples showing repair cartilage changes with occurrence of fibrocartilage tissue.

[Initial experience with implantation of the cemented Beznoska/S.V.L. total knee joint endoprosthesis].

Purpose Of The Study: The authors present the results of their first trial of a new, Beznoska/S. V. L.

[Arthroscopic reconstruction of the anterior cruciate ligament using the transtibial technique and a graft from the patellar ligament--results after 5-6 years].

Purpose Of The Study: Arthroscopic reconstruction of the anterior cruciate ligament (ACL) by transtibial technique with free graft from the patellar tendon fixed by interference screws is a commonly used procedure. The aim of this study is to evaluate the results 5 to 6 years after the surgery.

Material: Fifty-two of 79 patients operated on in 1995 were checked in the period of 2000-2001.

[Distal shortening osteotomy of the metatarsals using the Weil technique: surgical treatment of metatarsalgia and dislocation of the metatarsophalangeal joint].

Purpose: Weil technique of the distal shortening osteotomy of metatarsal is a relatively new method of the surgical treatment of metatarsalgia and dislocation of metatarsophalangeal (MTP) joints. A retrospective study evaluates the first experience in Weil osteotomy at the authors' department.

Material: Between May 1999 and the end of 2000 twelve patients (14 feet) were operated on in which the Weil technique of the osteotomy was performed on 28 metatarsals.

[The Zweymuller endoprosthesis in hip joint revision surgery].

Purpose Of The Study: The aim of the study is the verification of the applicability of the Zweymüller system of total hip replacement in dependence on the size of defects of acetabulum and femur and evaluation of early results in patients operated on in 1998 and 1999.

Material: In 1998 and 1999 we reimplanted 125 total hip replacements. In 60 revision surgeries we used at least one component of Zweymüller Bicon Plus system.

[Arthroscopic subacromial decompression.].

Arthroscopic subacromial decompression is a generally recognized method of the treatment of the primary impingement sydrome and is one of the basic arthroscopic methods. The data in literature present 73-94 % of good and very good results. From May 1993 till June 1996 we treated at our clinic 40 patients with arthroscopic subacromial decompression of which 38 patients were followed up.

[Artroskopická náhrada predního zkrízeného vazu volným stepem z ligamentum patellae transtibiální technikou.].

Surgical techniques of replacement of the anterior cruciate ligament were further developed in recent years. In the submitted paper the authors describe arthroscopic replacement of the anterior cruciate ligament by transtibial technique of a single incision with an incomplete femoral canal. The objective of the work is to test, based on the authors' own shortterm experience, the reliability and contribution of this technique and possibly correct it with regard to the assembled results and experience.

[Physical medicine and sports].

In the treatment of sportsmen where one of the essential requirements is a rapid and accurate diagnosis with effective therapy which reduces the period of rest and immobilization, knowledge from the sphere of manual medicine is a great asset. In addition to manipulation procedures proper which call for accurately defined conditions and practice, it is possible to apply a wide range of reflex methods and mobilization to achieve favourable therapeutic results in predominantly functional disorders of the locomotor apparatus. These efforts have their limitations and possibilities and in some instances it is necessary to seek in addition to impaired function also more serious diseases or damage caused by repeated microtraumas.

[Indications for and contribution of diagnostic arthroscopy of the knee joint].

The paper deals with indications and diagnostic possibilities of arthroscopy of the knee joint. The first part is devoted to the application of arthroscopy in the diagnosis of acute knee injuries. The Gillquists scheme of diagnostic procedure is outlined.