[Mass-spectrometric analysis of proteasomal subunits possessing endoribonuclease activity].

Proteasomes act as the main apparatus of non-lysosomal intracellular proteolysis and participate in the regulation of most important cellular processes. Despite considerable progress in the understanding of proteasome's functioning, some issues, in particular, RNase activity of these ribonucleoprotein complexes and its regulation remain scarcely explored. In this paper we found several proteins corresponding by electrophoretic mobility to subunits of the complex 20S proteasome to possess endoribonuclease activity with respect to both sense and antisense sequences of the c-myc mRNA 3'-UTR.

[Identification of rat and human hemoglobin acetilation sites after its interaction with acetylsalicylic acid].

The possibility of interaction of 0.1 mg/mL acetylsalicylic acid with purified human and rat globin in vitro during 24 h at 37 degrees C was investigated. The rat globin can be modified with acetylsalicylic acid on aminoacid residues K-17, K-57, K-91, K-140 in alpha subunit as well as on K-18, K-77 in beta subunit.

[Using of metal-affinity chromatography for identification of alkylated rat globin].

Rat globin peptides alkylated by sulfur mustard on amino-acid residues C-126, C-93 and E-27 with MH+ 1444.62 Da, 1561.66 Da, 1676.

[Analysis of nuclear protein complexes comprising alpha-actinin-4 by 2D-electrophoresis and mass-spectrometry].

Actin-binding protein alpha-actinin-4 is a member of spectrin super family. It is located in the cytoplasm and in the nucleus. However, nuclear functions of alpha-actinin-4 are still not clear.

[New approaches to early diagnosis of chronic organophosphorus chemicals intoxication in workers at chemical weapons extermination objects].

Mass spectrum analysis revealed differences in general contents of low-molecular peptides spectrums in chemical weapons extermination object staffers, in comparison with the reference group. Findings are that serum paraoxonase activity in chemical weapons extermination object staffers in significantly increased.

[Silicateins identification of the freshwater sponge L. baicalensis].

Siliceous spicules of the freshwater Baikal sponge Lubomirskia baicalensis contain several proteins including silicateins. Existences of four different genes of silicatein alpha (alpha1, alpha2, alpha3, alpha4) which are related to silicatein alpha from the sea sponges were found when cDNA library analysis was made. The intron-exon structure of the full-size silicatein alpha1 gene was determined.

[Interaction of laminin with the plasma membrane components of ascitic Zajdela hepatoma cells].

The laminin affinity chromatography was used for isolating laminin-binding proteins from the plasma membrane of Zajdela hepatoma cells synthesizing laminin. These were components with mol. weights about 80, 67, 60, 55, 52, 48 and 43 kDa.

[Revelation and identification of laminin in the structure of plasma membrane of ascitic Zajdela hepatoma cells].

Cell dysdifferentiation during neoplastic transformation is a crucial problem of cell biology and oncology. Antigenic diversion of cancer cells is a typical characteristic of dysdifferentiation. It involves the appearance of antigens which are unusual for normal tissue of this type.

[alpha-Actinin-4 and p65/RelA subunit of NF-kappaB transcription factor are co-localized and migrate together into the nucleus in EGF-stimulated A431 cell].

The NF-kappaB/Rel family of transcription factors in mammalian cells regulates inducible transcription of a large number of genes in response to diverse stimuli. Despite a great number of publications on this subject, little is known about precise NF-kappaB localization in the cytoplasm. As previously demonstrated, in normal rat fibroblast and human epidermoid carcinoma A431 cells p65/RelA subunit of NF-kappaB is co-localized in the cytoplasm with actin structures.

[Amplitude and time characteristics of latent auditory evoked potentials in occupational hearing disorders with and without the recruitment phenomenon].

Cortical evoked potentials (CEP) of workers exposed to industrial noise were recorded by an Amplaid MK-6 unit (Italy). They suffered from neurosensory hypoacusis with or without the recruitment phenomenon. The results were compared with those derived from patients with neurosensory hypoacusis of vascular origin, aggravated by the recruitment phenomenon, and in the cortical structures of the hearing organ in the case of neurosensory hypoacusis of both vascular and noise origin.

[Risk of damaging effect of noise on the hearing organ].

Hearing measurement of 6,784 workers exposed to industrial noise helped to determine the prevalence of occupation-related hypoacusis, which was estimated to vary from 4.5 to 53%. The work record of 10 to 14 years was found to be most risky for the development of cochlear neuritis.

[Morbidity with temporary disability among workers in the main professions at the machine-building plant].

The article contains the results of a profound analysis of morbidity with temporary disability among the workers of a machine-building plant's main shops. According to Dr E. L.

[Methodological principles of otorhinolaryngological ambulatory examinations and medical rehabilitation of workers exposed to intensive occupational noise and air pollutants].

This paper presents principles of otorhinolaryngological mass-scale examination of industrial workers exposed to noise and dust. They require not only early detection of pathologies of the hearing organ and upper airways but also assessment of the health status of each person and identification of groups at risk. Dynamic observation over healthy and sick subjects as well as proper application of prophylactic and therapeutic measures helps maintain good health and high performance of industrial workers.

[Indicators of the state of hearing and olfactory analyzers in healthy man].

This paper presents the concept of a biological norm, methods of experimental studies, and parameters of the hearing and olfactory function as derived from large-scale examinations of healthy people of different sex and age groups. On this basis parameters of the physiological norm for the hearing and olfactory function were calculated as applied to various age groups. The paper gives tables of average thresholds and limits of variations of hearing and olfactory sensitivity adapted for practical use.

[Improvement of primary and secondary prevention of occupational cochlear neuritis].

The methods of primary and secondary prophylaxis of cochlear neuritis in workers exposed to a high noise level are described. It is emphasized that hearing impairment can be prevented in a more efficient manner at the early stages: noise effect on the hearing organ and signs of the effect or mild symptoms of cochlear neuritis. A complete scheme of therapy is presented which includes vasodilators, vitamins, biostimulants, sedatives, physiotherapy.

[Precancerous conditions of the larynx in workers exposed to dust and their prevention].

This paper presents the results of examinations of 522 workers exposed to various industrial dusts and 361 workers exposed to different concentrations of carcinogenic asbestos dust. The examinations showed a significantly higher incidence of total processes in the upper respiratory tract which manifested as chronic subatrophic nasopharingitis and hyperplastic laryngitis. Also, they demonstrated a significantly higher incidence of local processes that manifested as hyperplasia of the laryngeal mucosa and contact fibromas.