[Interaction of C60 fullerene-polyvinylpyrrolidone complex and brain Aβ(1-42)-peptide in vitro].

Using a spectrophotometric method changes occurring in solution containing brain Aβ(1-42)-peptide, fullerene C60, and polyvinylpyrrolidone were analyzed. Using the Bent-French method relative binding constants of fullerene C60 with Aβ(1-42)-peptide and polyvinylpyrrolidone with Aβ(1-42)- peptide were determined. These data suggest that Aβ(1-42)-peptide interacting with the C60 fullerene-polyvinylpyrrolidone complex partially displaces polyvinylpyrrolidone and generates a new three molecular compound.

[Changes in gene expression and content of titin (connectin) in striated muscles of chronically etihanol-fed rats].

Changes in gene expression and isoform composition of giant sarcomeric protein titin (connectin) in cardiac muscle, as well as changes of its isoform composition in skeletal muscle (m. soleus) of chronically ethanol-fed rats have been studied using real-time RT-PCR and low percentage SDS-gel electrophoresis. The decrease of titin content in examined muscles and the decrease in titin gene expression in myocardium of chronically ethanol-fed rats have been shown.

[Seasonal changes in the isoform composition of the myosin heavy chains in skeletal muscles of hibernating ground squirrels Spermophilus undulatus].

Seasonal changes of the isoform composition of myosin heavy chains in skeletal muscles (m. triceps, m. longissimus dorsi, m.

[Antiamyloid properties of fullerene C60 derivatives].

A comparative estimation of the ability of complexes of fullerene C60 with polyvinylpyrrolidone and fullerene C60 derivatives (the sodium salt of the polycarboxylic derivative of fullerene C60, sodium fullerenolate), has been carried out. The fullerenes destroyed amyloid fibrils of the Abeta(1-42) peptide of the brain and the muscle X-protein. A study of the effect of fullerenes on muscle actin showed that complexes of fullerene C60 with polyvinylpyrrolidone and sodium fullerenolate did not prevent the filament formation of actin, nor did they destroy its filaments in vitro.

[Changes in the composition of cardiac muscle myosin light chains during cardiac diseases].

In this paper our data of the study on composition of human cardiac myosin light chains in norm and its changes at different stages of dilated cardiomyopathy and heart valvular diseases are presented. Functional role and diagnostic value of these changes are discussed.

[Role of light chains of myosin in the regulation of contraction of vertebrate striated muscles].

The data of the study on Ca2+ sensitivity of ATPase activity of myosin from vertebrate striated muscles in the presence of actin and the conditions of its manifestation and disappearance are presented. The role of Ca2+ sensitivity of actin-activated myosin ATPase in the regulation of contraction of vertebrate striated muscles is discussed.

[Fluorescence analysis of the action of soluble derivatives of fullerene C60 on amyloid fibrils of the brain peptide Abeta(1-42)].

It has been shown by fluorescence analysis in vitro that the water-soluble sodium salt of the polycarboxylic derivative of fullerene C60, fullerenol, and complexes of fullerene C60 with polyvinylpyrrolidone (mol. wt. 25000 and 10000) destroy amyloid fibrils of the brain peptide Abeta(1-42) and prevent their formation.

[Expression of titin in the myocardium of spontaneously hypertensive rats during in the course of hypertrophy].

Changes in the expression of N2B- and N2BA-isoforms of titin in the left ventricle of the myocardium of spontaneously hypertensive rats during the development of hypertrophy have been analyzed by the methods of real-time scale polymerase chain reaction and SDS gel electrophoresis. It was shown that, in early development of hypertrophy (15-week-old rats), an increase in the expression of mRNA of the titin gene and a decrease in the content of the protein itself occur. At a later stage of development (26-week-old rats), a decrease in the expression of titin at the level of both mRNA and the protein per se was observed.

[Effect of vibrostimulation on support zones of rat's feet, and support loading on titin N2A-isoform and T2-fragment in m. soleus under the conditions of simulated microgravity].

Effects of daily 3-hr vibrostimulation of foot support zone and 3 hrs. of usual locomotion on titin N2A-isoform and proteolytic T2-fragment were studied in m. soleus of rats during 7-d suspension.

[Effect of nitroderivatives of fullerene C60 on amyloid fibrils of Abeta(1-42) peptide of the brain and muscle X-protein].

The antiamyloidogenic capacity of water-soluble nitroderivatives of fullerene C60: methyl ester of LN-[(2-nitroglyceryl) fullerenyl] proline, methyl ester of LN-[(2,3-dinitroglyceryl) fullerenyl] proline, and 2 nitroxyethyl ether LN-([2-(nitroxy) ethyl] fullerenyl) proline has been studied in vitro by high-resolution electron microscopy. It was shown that these fullerene C60 nitroderivatives are able to prevent the formation of amyloid fibrils by the brain Abeta(1-42)-peptide and muscle X-protein and to destroy mature fibrils. Electron microscopy is a promising method for selecting effective antiamyloidogenic drugs.

[Contractile properties of fibers and cytoskeletal proteins of gerbil's hindlimb muscles after space flight].

The work had the goal to compare the microgravity effects on gerbil's muscles-antagonists, m. soleus and m. tibialis anterior.

[Effect of fullerenes C60 on the amyloids of X-protein].

The inhibitory effect of hydrated fullerene C60 (HyFn) and the sodium salt of the fullerene polycarboxylic derivative C60Cl (C6H4CH2COONa)5 on the formation of amyloid fibrils by X-protein in vitro has been studied by electron microscopy. It has been shown that these compounds not only destroyed mature amyloid fibrils but also prevented the formation of new fibrils. This property of fullerenes, nanoparticles, can be used for the development of a novel medicinal nanotechnology in the therapy of amyloidoses.

[Contractile properties of the isolated rat musculus soleus and single skinned soleus fibers at the early stage of gravitational unloading: facts and hypotheses].

The contractile properties of the postural rat soleus muscle at the early stage of the gravitational unloading (3-day rat hindlimb suspension) have been studied using different modes of muscle contraction (twitch and tetanic contraction of the isolated muscle, Ca-induced contraction of isolated skinned fibers). A significant enhancement of the twitch maximal tension of unloaded muscles without changes in time-dependent characteristics was observed, although the half-relaxation time tended to increase. The fiber diameter did not change (42.

[Seasonal changes in the composition of the titin isoforms in muscles of hibernating ground squirrels].

An electrophoretic study of changes in the content of intact titin isoforms, N2B-, N2BA-, N2A-titins and T2 in skeletal and cardiac muscles of ground squirrel (Spermophillus undulatus) in different periods: summer activity, autumn activity, hibernation, arousal, and winter activity has been carried out. In atriums and ventricles of autumn activity ground squirrels, an increase (approximately 1.5 times) in the N2BA-to-N2B ratio in comparison with that in cardiac muscle of summer activity ground squirrels has been observed.

[Composition of the titin isoforms in muscles in pathology].

An electrophoretic study of changes in the composition of titin isoforms in skeletal and cardiac muscles of human and rat in pathology has been carried out. A more considerable decrease in the content of intact titin isoforms in comparison with the content of N2BA- and N2B-titins in hypertrophic heart of spontaneously hypertensive rats, N2A-titin in back muscle from patients with the "stiff-man syndrome", and in m. soleus of human and rats during the development of "muscle hypogravity syndrome" has been observed.

[Structure and functions of titin, a giant protein of skeletal and cardiac muscles: evidence and suppositions].

The structural and functional properties of skeletal and cardiac titin are described according to the recent experimental evidence and suppositions.

[Autoantibodies to light myosin chains as an early marker of damage to the myocardium].

The concentration of autoantibodies to myosin light chains in the blood serum of patients who have undergone operation on heart under conditions of artificial hypothermal circulation was measured after 3, 6, 9, 18, 24, 36 h following the reperfusion of myocardium. According to the postoperative data, the patients were divided into three groups: patients with stable hemodynamics in the postoperative period (group 1); patients with myocardial stunning following reperfusion of myocardium (group 2); and patients with severe heart failure that developed in early postoperative period (group 3). Significant differences in the concentration of autoantibodies to myosin light chains have been found between the groups.

[Titin isoforms].

The titin isoform composition in skeletal and cardiac muscles of humans and animals has been studied using SDS elecrtophoresis in agarose-strengthened 1.3-2.3% polyacrylamide gels modified by us and immunoblot analysis in order to reveal new titin isoforms with molecular weights of more than 3700 kDa.

[Seasonal changes in phosphorylation of myosin regulatory light chains and C-protein in myocardium of hibernating ground squirrel Citellus undulatus].

A comparative study concerning the extent of phosphorylation of myosin regulatory light chains and C-protein from the left ventricle of hibernating ground squirrel Citellus undulatus during the periods of hibernation and activity was carried out. During hibernation, regulatory light chains of ground squirrel were found to be completely dephosphorylated. In active animals, the share of phosphorylated light chains averages 40-45% of their total amount.

[The C-terminal fragment of thymopoietin forms F-actin bundles: electron microscopic data].

It was shown by electron microscopy that PEP33 a synthetic C-terminal peptide of the thymus hormone thymopoietin, formed bundles of actin 'filaments in the presence of 0.1 M KCl. The structure of PEP33 aggregates localizated in the bundles between actin filaments is very similar to that of aggregates observed in samples of pure PEP33.

[Cytotoxicity of amyloid fibrils of X-protein].

It is known that amyloid oligomers, protofibrils, and fibrils induce cell death, and antibiotic tetracycline inhibits the fibrillization of beta amyloid peptides and other amyloidogenic proteins and disassembles their pre-formed fibrils. Earlier we have demonstrated that sarcomeric cytoskeletal proteins of the titin family (X-, C-, and H-proteins) are capable to form in vitro amyloid fibrils, and tetracycline effectively destroys these fibrils. Here we show that the viability of polymorphonuclear leukocytes in the presence of X-protein amyloids depends on the concentration of amyloid fibrils of X-protein and the time of incubation.

[Electron microscopic study of the influence of fullerene on the formation of amyloid fibrils by A beta(25-35) peptide].

The anti-amyloidogenic capacity of hydrated fullerene C60 HyFn was revealed by the use of electron microscopy. We first showed that when, connecting with growing amyloid fibrils formed by A beta(25-35)-peptide, fullerene prevented their subsequent growth and interfered with the formation of new fibrils. Instead of long helically twisted ribbons formed by A beta(25-35)-peptide in the absence of fullerene, short narrow protofibrils were found in the presence of fullerene .

[Creatine as a metabolic controller of skeletal muscles structure and function in strength exercises in humans. The cellular mechanisms].

The effects of creatine oral supplementation combined with a 10-week resistive training of morphometric, contractile and molecular characteristics of human vast lateral muscle fibers were studied. 2 groups consisting of 9 young healthy men each were involved in resistive training of knee extensors for 10 weeks. Volunteers of the first group received per os 20 g of creatine for the 1st week of training and 5 g for the rest of the experimental training period.

[Sarcomeric proteins of the titin family form amyloids].

It was shown for the first time that skeletal muscle sarcomeric proteins of the titin family (X-, C- and H-proteins) are able to form in vitro amyloid aggregates of different types: granular aggregates, protofibrils, helically twisted ribbons, linear fibrils, and bundles of linear fibrils. Their amyloid nature was confirmed by electron, polarization, and fluorescence microscopy and by spectral methods. As opposed to other amyloidogenic proteins, X-, C-, and H-proteins easily form amyloids under mild conditions close to physiological ones (pH, ionic strength, temperature).