Bis-conjugation of Bioactive Molecules to Cisplatin-like Complexes through (2,2'-Bipyridine)-4,4'-Dicarboxylic Acid with Optimal Cytotoxicity Profile Provided by the Combination Ethacrynic Acid/Flurbiprofen.

A facile route to Pt complexes doubly functionalized with bioactive molecules through a bipyridine-type ligand is described. Initially, ligands L (containing two ethacrynic acid units), L (ethacrynic acid+flurbiprofen) and L (ethacrynic acid+biotin) were obtained in moderate to good yields from 2,2'-bipyridine-4,4'-dicarboxylic acid. Subsequent reaction of the ligands with [PtCl (DMSO) ] afforded complexes [PtCl (L )] (2), [PtCl (L )] (3) and [PtCl (L )] (4) in high yields.

Manganese-doped green tea-derived carbon quantum dots as a targeted dual imaging and photodynamic therapy platform.

In this work, manganese-doped carbon quantum dots (Mn-CQDs) have been synthesized through a one-pot hydrothermal method by using waste green tea. The Mn dopants were introduced to impart magnetic resonance capability. Upon optimization of the experimental conditions, magnetofluorescent Mn-CQDs exhibit an excitation-dependent blue emission.

Synthesis of Cisplatin(IV) Prodrug-Tethered CuFeS Nanoparticles in Tumor-Targeted Chemotherapy and Photothermal Therapy.

In this study, for the first time, CuFeS nanocrystals were successfully prepared through a facile noninjection-based synthetic strategy, by reacting Cu and Fe precursors with dodecanethiol in a 1-octadecene solvent. This one-pot noninjection strategy features easy handling, large-scale production, and high synthetic reproducibility. Following hyaluronic acid (HA) encapsulation, CuFeS nanocrystals coated with HA (CuFeS@HA) not only readily dispersed in water and showed improved biocompatibility but also possessed a tumor-specific targeting ability of cancer cells bearing the cluster determinant 44 (CD44) receptors.

Sequential enzymatic derivatization coupled with online microdialysis sampling for simultaneous profiling of mouse tumor extracellular hydrogen peroxide, lactate, and glucose.

Probing tumor extracellular metabolites is a vitally important issue in current cancer biology. In this study an analytical system was constructed for the in vivo monitoring of mouse tumor extracellular hydrogen peroxide (HO), lactate, and glucose by means of microdialysis (MD) sampling and fluorescence determination in conjunction with a smart sequential enzymatic derivatization scheme-involving a loading sequence of fluorogenic reagent/horseradish peroxidase, microdialysate, lactate oxidase, pyruvate, and glucose oxidase-for step-by-step determination of sampled HO, lactate, and glucose in mouse tumor microdialysate. After optimization of the overall experimental parameters, the system's detection limit reached as low as 0.

Online open-tubular fractionation scheme coupled with push-pull perfusion sampling for profiling extravasation of gold nanoparticles in a mouse tumor model.

The extravasation of administered nano-drug carriers is a critical process for determining their distributions in target and non-target organs, as well as their pharmaceutical efficacies and side effects. To evaluate the extravasation behavior of gold nanoparticles (AuNPs), currently the most popular drug delivery system, in a mouse tumor model, in this study we employed push-pull perfusion (PPP) as a means of continuously sampling tumor extracellular AuNPs. To facilitate quantification of the extravasated AuNPs through inductively coupled plasma mass spectrometry, we also developed a novel online open-tubular fractionation scheme to allow interference-free determination of the sampled extracellular AuNPs from the coexisting biological matrix.