[Meta-analysis of experimental studies of the effect of melatonin monotherapy on the levels of thyroid hormones and glucocorticoids in rats kept under standard condition].

Background: Melatonin is known to modulate circadian and seasonal rhythms in metabolism, reproduction, and behavior. However, the effect of exogenous melatonin supplementation on the functioning of the thyroid and adrenal glands in species without a clear seasonality in reproduction is still unclear.

Aim: Using a meta-analysis of publications, to investigate the effect of melatonin monotherapy on the concentrations of pituitary thyroid-stimulating hormone, thyroid hormones (TG), pituitary adrenocorticotropic hormone and corticosterone (CS) in rats kept under standard laboratory conditions.

Seasonal dynamics of myocardial infarctions in regions with different types of a climate: a meta-analysis.

Background: It is known that cardiovascular events (CVE) occur more often in winter than in summer. However, dependence of myocardial infarction (MI) risk of on various meteorological factors is still not fully understood. Also, the dependence of the seasonal dynamics of MI on gender and age has not yet been studied.

Seasonal variations in levels of human thyroid-stimulating hormone and thyroid hormones: a meta-analysis.

Seasonal dynamics in biological functions of mammals is regulated by melatonin-mediated circannual fluctuations in the secretion of thyroid-stimulating hormone (TSH) and thyroid hormones. Most anatomical and molecular structures responsive to photoperiod and melatonin secretion changes and the associated receptors are preserved in modern humans. This work aimed to determine the seasonal dynamics of TSH and thyroid hormone levels (total triiodothyronine (T3), free triiodothyronine (FT3), thyroxine (T4), free thyroxine (FT4) and to investigate the dependence of these variations on gender, age and amplitude of meteorological fluctuations.

Size-Dependent Bioactivity of Silver Nanoparticles: Antibacterial Properties, Influence on Copper Status in Mice, and Whole-Body Turnover.

Purpose: The ability of silver nanoparticles (AgNPs) of different sizes to influence copper metabolism in mice is assessed.

Materials And Methods: AgNPs with diameters of 10, 20, and 75 nm were fabricated through a chemical reduction of silver nitrate and characterized by UV/Vis spectrometry, transmission and scanning electronic microscopy, and laser diffractometry. To test their bioactivity, cells, cultured A549 cells, and C57Bl/6 mice were used.

[Changes in the autonomic control of the cardiovascular system in human aging. meta-analysis.].

It is known that with the aging, the adaptive capabilities of his body decrease. This is due to both changes in autonomic regulation and morphological remodeling of the cardiovascular system. To assess age-related changes in the autonomic nervous and cardiovascular systems, a meta-analysis was carried out, which examined changes in the aging of hemodynamic parameters [blood pressure (BP) and heart rate (HR)], heart rate variability (HRV), catecholamine concentrations, electrical activity of muscle sympathetic nerves (MSHA) , as well as the magnitude of the BR.

Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion.

Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo.

[Dose-dependent effects of melatonin on the functioning of the cardiovascular system and on the behavior of normotensive rats of different ages.].

The purpose of the work on normotensive rats of different age groups (3, 15 and 22 months) to study the synchronism between the functioning of the cardiovascular system and the locomotor activity of animals in the «open field» test by a single injection of exogenous melatonin in different doses (1 and 10 mg/kg). Studies have revealed the unidirectional dose-dependent effect of exogenous melatonin on the locomotor activity of rats of different ages and the age-dependent effect of melatonin on the parameters of the cardiovascular system. It shows the possibility desynchronization of the circadian rhythms of locomotor activity and the functioning of the cardiovascular system with aging, which can lead to a discrepancy between hemodynamic parameters and the level of locomotor activity.

[Effects of hyper- and hypothermia on hemodynamic parameters in people of different age groups. Meta-analysis.].

Age-related changes in thermoregulation occur due to the deterioration of the adaptive capacity of the cardiovascular system caused by remodeling of the vascular wall and myocardium, capillary density reduction, changes in the synthesis and clearance of neurotransmitters and vasoactive substances, as well as the inhibition of the vegetative mechanisms of hemodynamic parameters regulation. A common way to simulate hyper- and hypothermal conditions is to use a suit with tubes to fill with water at different temperatures. The purpose of this work is to conduct a meta-analysis of the hemodynamic parameters shifts when modeling hyper- and hypothermia using such a suit in people of different age groups.

Can the outcomes of mesenchymal stem cell-based therapy for myocardial infarction be improved? Providing weapons and armour to cells.

Use of mesenchymal stem cell (MSC) transplantation after myocardial infarction (MI) has been found to have infarct-limiting effects in numerous experimental and clinical studies. However, recent meta-analyses of randomized clinical trials on MSC-based MI therapy have highlighted the need for improving its efficacy. There are two principal approaches for increasing therapeutic effect of MSCs: (i) preventing massive MSC death in ischaemic tissue and (ii) increasing production of cardioreparative growth factors and cytokines with transplanted MSCs.

[ADRENERGIC AND IMIDAZOLINE MECHANISMS OF CIRCULATION CENTRAL REGULATION IN RATS OF DIFFERENT LINES].

The purpose of this work was a comparative analysis of the brain adrenergic and imidazoline receptors activation effects in laboratory rats with normal and elevated activity of the sympathetic nervous system. The experiments were carried out on male rats of Wistar and Wistar-Kyoto lines and spontaneously hypertensive (SHR) line. In freely moving rats the mean arterial pressure, heart beat-to-beat interval and cardiochronotropic component of baroreceptor reflex were recorded and analyzed at rest and emotional stress, caused by a conditioned signal.

[Melatonin in complex treatment for colorectal cancer patients].

Colorectal cancer is closely related to changes in the immune status at all stages of the disease. The main method of treatment for colorectal cancer is surgery. Surgical intervention as any pathological impact on the body from the outside is leading to suppression of both humoral and cellular immunity.

[IMMUNE RESPONSE IN PATIENTS WITH COLORECTAL CANCER IN POSTOPERATIVE PERIOD].

It was shown by the authors that changes of the level of cytokines reflected the degree of invasiveness of operative intervention. The endovideosurgical approach was less traumatic and provided a rapid rehabilitation of the patients in postoperative period. It is possible to consider the high levels of IL-10 as a predictor of development of local inflammatory process and as an indicator of probable infectious complications in postoperative period.

[ASSESSMENT OF EFFICACY OF MELATONIN IN SURGICAL TREATMENT OF COLON TUMORS].

Melatonin is neurohormone, which is involved in regulation of many functions of an organism, including the digestive system. Therefore the authors offered to include this hormone as a preconditioner factor in surgical treatment of colon tumors using laparotomy and laparoscopy. Preoperative application of melatonin allowed shortening the terms of postoperative period and hospital stay.

The effects of silver ions on copper metabolism in rats.

The influence of short and prolonged diet containing silver ions (Ag-diet) on copper metabolism was studied. Two groups of animals were used: one group of adult rats received a Ag-diet for one month (Ag-A1) and another group received a Ag-diet for 6 months from birth (Ag-N6). In Ag-A1 rats, the Ag-diet caused a dramatic decrease of copper status indexes that was manifested as ceruloplasmin-associated copper deficiency.

[Experimental study of the effect of benzidinsulfon, included in the group of diaminodifenils, with potential carcinogenic effects].

There was performed a study of carcinogenicity of benzidinsulfon (4.4'-diaminodiphenil sulfone) in rats and mice. Experimental animals (99 mice and 99 rats, approximately equally divided by sex) received the drug throughout the life by subcutaneous injections (once a week) or addition to food (5 times a week).

Arterial baroreceptor reflex counteracts long-term blood pressure increase in the rat model of renovascular hypertension.

Introduction: The present study tested the hypothesis that long-term effects of baroreceptor activation might contribute to the prevention of persistent arterial blood pressure (BP) increase in the rat model of renovascular hypertension (HTN).

Methods: Repetitive arterial baroreflex (BR) testing was performed in normo- and hypertensive rats. The relationship between initial arterial BR sensitivity and severity of subsequently induced two-kidney one-clip (2K1C) renovascular HTN was studied in Wistar rats.

[Somatosympathetic reflex in rats with various models of arterial hypertension].

Spontaneous and reflex activities of sympathetic nerve were compared in animals with arterial hypertension of different aetiology. Reflex discharges elicited by single-shock stimulation of afferent fibres were recorded. In acute experiences on anaesthetized rats with renovascular and spontaneous (SHR) model of arterial hypertension, electric basal and evoked activity (somatosympathetic reflex) in cervical sympathetic trunk were recorded.

[Baroreceptor reflex and arterial hypertension].

The vaso-renal arterial hypertension was produced in in the "two kidneys-one clip" model in experiments on Wistar male rats. This procedure was performed both in rats with intact mechanoreceptor zones of large vessels and the rats with destroyed baroreceptors. The date obtained indicate that in rats with cutting of "buffer" nerves the arterial hypertension after clipping renal artery occurred in all rats while in rats with intact sino-carotid and aortal nerves arterial hypertension developed only in 39% of the animals.

[The role of the central nervous system imidazoline receptors in cardiovascular manifestations of emotional stress].

The effect of central nervous system imidazoline receptors activation on basal blood pressure level, heart rate and arterial baroreceptor reflex in steady state and aversive emotional tension was tested in experiments on alert WKY, SHR and white bastard rats. It was found that the brain imidazoline receptors activation led to arterial baroreceptor reflex rise (both in resting and in emotional tension) and caused an emotional stress pressor effects decrease. No data proving involvement of imidazoline receptors in functioning of the systems maintaining level of blood pressure, were found.

[Use of Azopiram for rapid diagnosis of epithelial tumors of the urinary bladder (experimental data)].

Correlation of development of tumor pathology and macro- and microhematuria was investigated in the experiments using 50 male rats with N-butyl-N-(4-hydroxybutyl)-nitrosoamine-induced tumors of the urinary bladder. Our highly-sensitive agent Azopiram was employed to monitor hematuria. Microhematuria, which subsequently developed into macrohematuria as tumor matured and turned malignant, was identified as early as at such incipient neoplastic changes as focal hyperplasia of the urothelium and micropapillomatosis.

[Cardiovascular responses to emotional stress during activation of the brain imidazoline receptors].

In alert normotensive and hypertensive (SHR) rats, effects of imidazoline receptor activating compounds of the central nervous system on the level of blood pressure, heart rate and value of arterial baroreceptor reflex at rest and in emotional tension, were investigated. It was shown, that the activation of the cerebral imidazoline receptors leads to an increase in the baroreceptor reflex value (both in resting and in emotional tension) and a decrease in the pressor reaction evoked by emotional affect. No data showing the role of imidazoline receptors in functioning of systems regulating of the initial level of blood pressure were obtained.

[Arterial mechanoreceptor reflex as a factor for execution of hypertensive responses to disturbing effects].

It has been shown in experiments on conscious cats and rats that arterial mechanoreceptor reflex is inhibited during averse emotional influence, and the changed reflex is a cause of elevation in arterial pressure. In one's turn the hypertension contributes to entry of blood plasma norepinephrine into brain and in this way increases the arterial baroreceptor reflex after the emotional influence is stopped. The augmentation of baroreflex promotes restoration of homeostasis after cessation of the stress situation.

[The possible carcinogenic action of a protein concentrate from Saccharomyces yeasts grown on molasses].

A testing was done in a chronic experiment on 300 rats and 360 mice of both sexes for carcinogenic potential of a new protein product from Saccharomyces yeast grown in melasse. The production procedures and techniques of the above product have been worked out at the Ukrainian Research Institute of Spirits and Biotechnology of Food Stuffs of Gospishcheprom (State Food Industry) of Ukraine. The studies made showed the new protein product has no carcinogenic effect.

[The mechanisms regulating the value of the arterial pressure in alert rats].

N-g-nitro-L-arginine increased the mean arterial pressure in intact alert rats against the background of phenoxybenzamine and captopryl, as well as benzohexonium. The findings suggest that systems of short-term blood-pressure control are limited by the sympatho-adrenal vasopressin and renin-angiotensin systems.