[Experience of surgical care administration to patients with closed abdominal trauma, serving on the ships of the Navy].

The analysis of surgical care administration to personnel, serving on ships of the Navy of Russia and performing different tasks in off-shore maritime and ocean zones in 2012-2013 showed that there is a requirement to create seven additional ship groups of specialized medical care: on the Northern Fleet--2, on the Pacific Fleet--2, on the Baltic Fleet--1, on the Black Sea Fleet--1, on the Caspian Flotilla--1. There is also a reasonable requirement to include anaesthesiologist and nurse anaesthetist into these groups.

[Delivery of surgical care on naval ships: formation, development, and current stage].

Acute surgical diseases were and remain one of the most important problems of the organization of medical care and treatment of patients in the conditions of long distant sea voyage, when there is no possibility for medical evacuation. We analyzed the positive experience of surgical care in the sea, gained by Soviet and then and by Russian Navy physicians. As we haven't registered significant changes in morbidity of Navy crewmembers, we think that studying and creative application of this experience will have the positive effect.

[Health, morbidity and injury rate among the north fleet seafaring personnel].

Health analysis of the basic and contractual servicemen of varying age was performed using the data of multi-year observations of personnel of a surface naval unit in the Kola arctic region. The article presents analysis of seamen health, level and structure of primary morbidity, structure and causes of injuries, distribution by the ICD-10 codes of disease, and data of regular medical check-ups. The term of comorbidity has been proposed to analyze morbidity among the servicemen and its dependence on environmental and occupational factors.

[Regulation of proliferation and viability of tumor cells in vitro by alloferon-1 and allostatin-1].

Alloferon-1 (AF) and allostatin-1 (AS) cytotoxic and growth modulating activities have been compared. AF is cationic oligopeptide isolated from the hemolymph of experimentally infected blow fly Calliphora vicina. AS is AF synthetic analog that differs from the parent molecule in two amino acids substituted.

[Growth modulating and cytotoxic effects of the peptide from hemolymph of blow fly Calliphora vicina (Diptera, Calliphoridae) in vitro].

Biological activity of alloferon (AF), peptide, consisting of 13 a. a. isolated from hemolymph of experimentally infected blow fly Calliphora vicina was studied.

Antiviral and antitumor peptides from insects.

Insects can rapidly clear microbial infections by producing a variety of immune-induced molecules including antibacterial and/or antifungal peptides/polypeptides. In this report, we present the isolation, structural characterization, and biological properties of two variants of a group of bioactive, slightly cationic peptides, referred to as alloferons. Two peptides were isolated from the blood of an experimentally infected insect, the blow fly Calliphora vicina (Diptera), with the following amino acid sequences: HGVSGHGQHGVHG (alloferon 1) and GVSGHGQHGVHG (alloferon 2).

[Cytotoxic and mitogenic effect of antimicrobial peptides from neutrophils on cultured cells].

Cytotoxic and mitogenic activities of human and rabbit defensines (HNP and NP-2, resp.) and pig antimicrobial peptides from leukocytes (PR-39, prophenin PF-2 and protegrin PG-2) were studied. The above peptides were added to serum-free cell culture medium of the target cell lines K562, L929 and Hep22a.

[The immunomodulating and immunoprotective activity of small ribonucleoprotein complexes (alpha RNPs) from the LRec-1sf cell line].

Effect of nuclear and released into culture medium alpha RNPs (N- and R-alpha-RNPs, resp.) produced by transformed rat embryo fibroblasts of serum-free cell line LRec-1sf on the nonsensibilized mouse splenocyte cytotoxicity (NK-mediated cell lysis) was studied. A preliminary treatment with N-alpha-RNPs resulted in decreasing K562 cell sensitivity to splenocyte cytotoxicity, whereas pretreatment of the splenocytes themselves exerted no cytotoxic effect.

[The release and absorption of small ribonucleoprotein complexes (alpha RNPs) in cultures of transformed rat fibroblasts and human epidermoid carcinoma].

The release of alpha RNPs and their absorbtion by the cells from culture medium were studied. The rat fibroblasts of parental serum-dependent cell line LRec-1, and of selected serum-free cell line LRec-1sf rapidly released and absorbed alpha RNPs. In the latter case, both auto- and heterologous alpha RNPs were seen to penetrate, whereas only autologous alpha RNPs entered LRec-1 cells.

[The growth-stimulating and immunomodulating activity of culture media conditioned by transformed fibroblasts from serum-free lines].

Five cell lines of transformed rodent fibroblasts capable of unlimited growth without exogenous proteins were studied. It has been demonstrated that the media conditioned by these cells (CMs), the protein preparations obtained from CMs, or the feeder cell cultures of the serum-free cell lines are mitogenic to mouse hybridoma cells as well as to primary cell cultures of the rat bone marrow. The addition of CMs proteins into mixtures of mouse splenocytes and transformed target cells resulted in stimulation or suppression of the splenocyte cytotoxicity when YAC-1 [correction of JAC-1] or K562 cell targets were used.

[The cultivation features, growth characteristics and oncogenicity of "serum-free" populations of transformed fibroblasts].

The authors report about five previously selected cell lines of transformed mouse and rat fibroblasts, capable of growing in the medium lacking exogenous proteins. In contrast to cells of the parental lines, the cells under investigation have the ability to semi-suspension growth pattern and demonstrate an increasing sensitivity to damaging action of trypsin. The lowering of the autological conditioned medium (CM) concentration below 10-15%, as well as the reducing of the initial population density to 0.

[The isolation and characteristics of monoclonal antibodies to the HNK-1 antigen of natural killers].

Monoclonal antibodies to the HNK-1 differentiated antigen of natural killer cells have been obtained. A glycoprotein of the white human brain connected with myelin was used as an antigen for immunization of mice. The monoclonal antibodies obtained are shown to reduce the cytotoxic activity of a human blood mononuclear fraction as much as by 65% in relation to the human lymphoblastoma cell culture K-562.

[The autocrine regulation of proliferation in cell cultures. I. A study of the heparin-binding factors with growth-stimulating activity contained in media conditioned with transformed rat fibroblasts].

A study was made of the medium conditioned by spontaneously transformed rat embryo fibroblasts of line Rec1-sf, which are capable of unlimited reproduction in medium free of serum and of other endogenous growth factors (c-medium). Addition of c-medium to stationary cultures of nontransformed rat embryo fibroblasts (REF), spontaneously transformed REF (line Rec1), and cells of Rec1-sf stimulated the incorporation of 3H-thymidine by 1.5-6 times.

[The regulation of splenocyte natural killer activity in C3HA mice in the early period after hepatoma 22a transplantation].

A decrease of the natural killer activity (NKA) of C3HA splenocytes after transplantation of hepatoma 22a cells was connected to some extent with the suppressor action of macrophages and T-lymphocytes. Elimination of the macrophages from the splenocyte suspension led to the increase of NKA. Addition of the macrophages to the nonadherent cells resulted in the NKA inhibition to the level of nonfractionated splenocytes.

[Effect of teturam on the testosterone level and spermatogenesis in patients with alcoholism].

Disulfiram is one of the most common agents used for the treatment of alcoholism. A study was made of disulfiram influence on the level of testosterone and spermiogenesis in 20 alcoholic patients during course therapy. The level of testosterone was investigated before treatment and 15 and 30 days after treatment with disulfiram at a daily dose of 0.

[Natural killer activity and sensitization of the lymphocytes to tissue antigens in lung cancer].

A decreased natural killer activity was observed in patients with lung cancer as the disease progressed. Surgical intervention results in its elevation at the IIId stage, while the application of cytostatics or irradiation are efficient at the IIId and IInd stages. Pronounced sensibilization of the peripheral blood lymphocytes in cancer patients to normal and cancer tissue antigens was demonstrated with the help of the leukocyte adhesion inhibition test.

[Natural killer activity of splenocytes of C3HA mice in the early period after transplantation of hepatoma 22a cells].

It is shown that during 10 days after transplantation of syngenic 22a hepatoma cells to C3HA mice, wave-like fluctuations of the natural killer cell (NK) activity of splenocytes take place. Xenogenic (K-562) and allogenic (AC-1) cells are used as target cells in the 18th hour 3H-uridine cytotoxic test. A significant increase in the NK-activity on the 2nd-3rd and 7-9th days after tumour cells transplantation is observed.

[Sensitization of murine splenocytes to normal tissue antigens and natural killer activity. II. The effect of immunization with allogeneic and xenogeneic tissues].

Immunization of C3HA mice with allogeneic tissue (liver of DBA mice) leads to wave-like variability in the natural killer activity of their recipient splenocytes. This variability is similar to that of NK-activity due to syngeneic immunization and is characterized by a sharp increase on days 1, 3-4 and 6 after immunization. Xenogeneic immunization exerts no influence on NK-activity, if compared with the injection of 0.

[Control of cell multiplication in vitro. V. The dependence of cell multiplication on the ganglioside content].

The concentration and composition of gangliosides in the cells of L-line and its clones--L-2 and Lebr 1/1, with values of saturation densities being 0.9, 1.6 and 3.

[Cyclic adenosine monophosphate and the enzyme activity of its metabolism in L cells sensitive and resistant to the cytotoxic action of ethidium bromide].

Using L-cells both sensitive and resistant to cytotoxic action of ethidium bromide (EB), a study was made of the intracellular level of cAMP, activities of adenylcyclase, phosphodiesterase and cAMP, liberated from cells into the surrounding medium. In EB resistant L-cells compared to EB sensitive ones, the higher level of cAMP with a decreased activity of adenylcyclase and an increased activity of the phosphodiesterase was shown to be associated with an impeded exit of cAMP from cells. It is suggested that the differences in cAMP levels in the EB sensitive and resistant cells are associated with the properties of cAMP-dependent protein kinases of these cells.

[Control of cell multiplication in vitro. II. Relationship between cell multiplication and cyclic AMP concentration].

Compairing non-transformed mouse fibroblasts and some L-cell variants, it was shown that the decrease of dependence on serum mitogenic factors, and the decease of sensitivity to the contact inhibition of growth do not always correlate with the decrease of intracellular cAMP content. The decrease of sensitivity of transformed cells to the factors limiting non-transformed cell growth in vivo might be also connected with a relative independence of the cell growth on the intracellular level of a cAMP.

[Control of cell proliferation in vitro. I. Relation of proliferation to blood serum factors].

The population of L-cells sensitive to the cytotoxic action of ethidium bromide (EB) has at least 2 cell types differing in some growth characteristics and in their dependence on serum factors, controlling saturation density of culture. L-cells, resistant to EB, differ from sensitive cells in many features studied and reveal the ability for a continuous and, probably, unlimited multiplication in serum-free medium.