Constructed wetland as a green remediation technology for the treatment of wastewater from underground coal gasification process.

The wastewater from underground coal gasification (UCG) process has extremely complex composition and high concentrations of toxic and refractory compounds including phenolics, aliphatic and aromatic hydrocarbons, ammonia, cyanides, hazardous metals and metalloids. So, the development of biological processes for treating UCG wastewater poses a serious challenge in the sustainable coal industry. The aim of the study was to develop an innovative and efficient wetland construction technology suitable for a treatment of UCG wastewater using available and low-cost media.

Exudates from Miscanthus x giganteus change the response of a root-associated Pseudomonas putida strain towards heavy metals.

The composition of root exudates is modulated by several environmental factors, and it remains unclear how that affects beneficial rhizosphere or inoculated microorganisms under heavy metal (HM) contamination. Therefore, we evaluated the transcriptional response of Pseudomonas putida E36 (a Miscanthus x giganteus isolate with plant growth promotion-related properties) to Cd, Pb and Zn in an in vitro study implementing root exudates from M. x giganteus.

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study.

The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined.

Hybrid nanolayers of star polymers and silver nanoparticles with antibacterial activity.

The aim of this study was to obtain stable star polymer layers with incorporated silver nanoparticles (AgNPs) and to study the antimicrobial activity of these hybrid materials. In this work, a novel approach regarding the synthesis of AgNPs directly by the star polymer layer is presented. Nanolayers of poly(N,N'-dimethylaminoethyl methacrylate) and hydroxyl-bearing poly[oligo(ethylene glycol) methacrylate] (P(DMAEMA-co-OEGMA-OH)) stars, covalently bound with solid supports, were obtained through chemical reaction of hydroxyl groups in the star arms with substrate modified with imidazole derivative.

Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study.

The present study was focused on the identification of multi-resistant bacteria from the WHO priority pathogens list in the samples taken from different stages of the full-scale municipal wastewater treatment plant and receiving water. Additionally, the seasonal variations of the selected multi-resistant pathogens were analyzed in the samples. In order to the aim of the study, the metagenomic DNA from the collected samples was isolated and sequenced.

Industrialization as a source of heavy metals and antibiotics which can enhance the antibiotic resistance in wastewater, sewage sludge and river water.

The spread of antibiotic resistance is closely related with selective pressure in the environment. Wastewater from industrialized regions is characterized by higher concentrations of these pollutants than sewage from less industrialized areas. The aim of this study was to compare the concentrations of contaminants such as antibiotics and heavy metals (HMs), and to evaluate their impact on the spread of genes encoding resistance to antimicrobial drugs in samples of wastewater, sewage sludge and river water in two regions with different levels of industrialization.

Insights into the microbial diversity and structure in a full-scale municipal wastewater treatment plant with particular regard to Archaea.

Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream discharge point). Our study was focused on showing how the treatment processes influenced the Eubacteria and Archaea composition. Alpha and Beta diversity were used to evaluate the microbial diversity changes in the collected samples.

Whole-Genome Sequences of Antibiotic-Resistant Aeromonas caviae Strains Isolated from Treated Wastewater.

The presented data provide new information on antibiotic resistance and virulence genes in the genomes of strains TW-2 and TW-6, isolated from treated wastewater. The results confirm the presence of multi-antibiotic-resistant strains with virulence properties as "high-risk isolates" in treated wastewater.

Surfactin as a Green Agent Controlling the Growth of Porous Calcite Microstructures.

This study presents a new, simple way to obtain mesoporous calcite structures via a green method using an eco-friendly surface-active compound, surfactin, as a controlling agent. The effects of synthesis time and surfactin concentration were investigated. The obtained structures were characterized by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) coupled with gas mass spectrometry (QMS) analysis.

Effect of the freeze-drying process on the phenotypic diversity of Pseudomonas putida strains isolated from the interior of healthy roots of Sida hermaphrodita: Phenotype microarrays (PMs).

The objective of this study was to research the effect of the freeze-drying process on the metabolic changes of Pseudomonas putida strains (E41, E42, R85) isolated from the interior of Sida hermaphrodita roots with the use of the phenotypic microarrays (PM) technology. The proposed method of the freeze-drying process with inulin as component lycoprotectant demonstrated a high bacterial survival ratio (BSR) immediately after freeze-drying and storage after 12 months. While, after 360 days of freeze-drying BSR decreased to value of 74.

Fluoroquinolone Resistance and Virulence Properties Among Wastewater Isolates.

The study provides data on antibiotic resistance as well as the virulence characteristics of isolated from raw and treated wastewater. The isolates were identified as by 16S rRNA gene sequencing. In the analyzed strains, high frequency for the following genes was observed: , B, and D.

Biosurfactants: Eco-Friendly and Innovative Biocides against Biocorrosion.

Corrosion influenced by microbes, commonly known as microbiologically induced corrosion (MIC), is associated with biofilm, which has been one of the problems in the industry. The damages of industrial equipment or infrastructures due to corrosion lead to large economic and environmental problems. Synthetic chemical biocides are now commonly used to prevent corrosion, but most of them are not effective against the biofilms, and they are toxic and not degradable.

Environmental Risk and Risk of Resistance Selection Due to Antimicrobials' Occurrence in Two Polish Wastewater Treatment Plants and Receiving Surface Water.

In this study, a screening of 26 selected antimicrobials using liquid chromatography coupled to a tandem mass spectrometry method in two Polish wastewater treatment plants and their receiving surface waters was provided. The highest average concentrations of metronidazole (7400 ng/L), ciprofloxacin (4300 ng/L), vancomycin (3200 ng/L), and sulfamethoxazole (3000 ng/L) were observed in influent of WWTP2. Ciprofloxacin and sulfamethoxazole were the most dominant antimicrobials in influent and effluent of both WWTPs.

Nanolayers of Poly(,-Dimethylaminoethyl Methacrylate) with a Star Topology and Their Antibacterial Activity.

In this paper, we focus on the synthesis and characterization of novel stable nanolayers made of star methacrylate polymers. The effect of nanolayer modification on its antibacterial properties was also studied. A covalent immobilization of star poly(,'-dimethylaminoethyl methacrylate) (PDMAEMA) to benzophenone functionalized glass or silicon supports was carried out via a "grafting to" approach using UV irradiation.

Changes induced by heavy metals in the plant-associated microbiome of Miscanthus x giganteus.

Miscanthus x giganteus is a high biomass producing plant with tolerance to heavy metals. This makes Miscanthus interesting to be used for phytoremediation of heavy metal contaminated areas coupled with energy production. Since plant performance in metal polluted areas is impaired, their growth and phytoremediation effect can be improved with bacterial assistance.

Surfactants of microbial origin as antibiofilm agents.

The microbial world provides new energy sources and many various 'green' chemicals. One type of chemicals produced by microorganisms is the biosurfactant group. Biosurfactants are universal molecules, exhibiting surface properties often accompanied by desired biological activity.

Characterization of Extracellular Biosurfactants Expressed by a Pseudomonas putida Strain Isolated from the Interior of Healthy Roots from Sida hermaphrodita Grown in a Heavy Metal Contaminated Soil.

Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant derived from P.

Effect of a lipopeptide biosurfactant on the precipitation of calcium carbonate.

Observing flora and fauna, it can be said that nature is a great architect. Nature can create amazing structures with unique properties that may find potential applications in industry. This phenomenon is why the biomimetic synthesis of calcium carbonate with various polymorphs, sizes and morphologies using natural biomolecules, such as proteins and polysaccharides, has become an interesting topic in recent years.

Properties of Antibiotic-Resistant Bacteria Isolated from Onsite Wastewater Treatment Plant in Relation to Biofilm Formation.

The aim of the present study was to determine some properties of antibiotic-resistant bacterial strains isolated from onsite wastewater technology in relation to biofilm formation, e.g., autoaggregation and motility.

Structural identification of lipopeptide biosurfactants produced by Bacillus subtilis strains grown on the media obtained from renewable natural resources.

The aim of the study was to identify and characterize lipopeptide (LP) biosurfactants produced by two Bacillus subtilis strains (KP7 and I'-1a) grown on various media prepared from renewable natural resources: two different brewery wastewaters (BW#4 and BW#6), 2% beet molasses (M), apple peels extract (APE) supplemented with 0.25% of yeast extract (YE) or 0.25% peptone (P), and similarly supplemented carrot peels extract (CPE).

Complete Genome Sequences of Two Plant-Associated Isolates with Increased Heavy-Metal Tolerance.

We report here the complete genome sequences of two isolates recovered from surface-sterilized roots of The two isolates were characterized by an increased tolerance to zinc, cadmium, and lead. Furthermore, the strains showed typical plant growth-promoting properties, such as the production of indole acetic acid, cellulolytic enzymes, and siderophores.

Using phenotype microarrays in the assessment of the antibiotic susceptibility profile of bacteria isolated from wastewater in on-site treatment facilities.

The scope of the study was to apply Phenotype Biolog MicroArray (PM) technology to test the antibiotic sensitivity of the bacterial strains isolated from on-site wastewater treatment facilities. In the first step of the study, the percentage values of resistant bacteria from total heterotrophic bacteria growing on solid media supplemented with various antibiotics were determined. In the untreated wastewater, the average shares of kanamycin-, streptomycin-, and tetracycline-resistant bacteria were 53, 56, and 42%, respectively.