Changing pattern of dengue virus serotypes in Thailand between 2004 and 2010.

Dengue virus infection is a major concern in several countries, and more than 50 million people are infected worldwide each year. Thailand is one of the countries where people are susceptible to infection due to favourable geographical and environmental conditions. In this retrospective study, we reported the changing pattern of dengue virus serotypes during the period between 2004 and 2010.

Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus.

Objective: To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.

Methods: We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.

Results: The assay's sensitivity was 97.

Evaluation of rapid immunochromatographic NS1 test, anti-dengue IgM test, semi-nested PCR and IgM ELISA for detection of dengue virus.

Dengue virus (DENV) causes various clinical symptoms of differing severity based on time of infections. The existing laboratory methods, semi-nested PCR and Dengue IgM ELISA, still have limitations for diagnosis. A commercially available rapid immunochromatographic dengue NS1 antigen and IgM antibody tests in comparison with semi-nested PCR and IgM ELISA for confirmation of DENV infection were evaluated.