Caged Zn Photolysis in Zebrafish Whole Brains Reveals Subsecond Modulation of Dopamine Uptake.

Free, ionic zinc (Zn) modulates neurotransmitter dynamics in the brain. However, the sub-s effects of transient concentration changes of Zn on neurotransmitter release and uptake are not well understood. To address this lack of knowledge, we have combined the photolysis of the novel caged Zn compound [Zn(DPAdeCageOMe)] with fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes in live, whole brain preparations from zebrafish ().

Dopamine Release Impairments Accompany Locomotor and Cognitive Deficiencies in Rotenone-Treated Parkinson's Disease Model Zebrafish.

In this work, we carried out neurochemical and behavioral analysis of zebrafish () treated with rotenone, an agent used to chemically induce a syndrome resembling Parkinson's disease (PD). Dopamine release, measured with fast-scan cyclic voltammetry (FSCV) at carbon-fiber electrodes in acutely harvested whole brains, was about 30% of that found in controls. Uptake, represented by the first order rate constant () and the half-life () determined by nonlinear regression modeling of the stimulated release plots, was also diminished.

Impaired Dopamine Release and Latent Learning in Alzheimer's Disease Model Zebrafish.

Alzheimer's disease (AD) is a progressive, fatal, neurodegenerative disorder for which only treatments of limited efficacy are available. Despite early mentions of dementia in the ancient literature and the first patient diagnosed in 1906, the underlying causes of AD are not well understood. This study examined the possible role of dopamine, a neurotransmitter that is involved in cognitive and motor function, in AD.

Characterization of D3 Autoreceptor Function in Whole Zebrafish Brain with Fast-Scan Cyclic Voltammetry.

Zebrafish () are ideal model organisms for investigating nervous system function, both in health and disease. Nevertheless, functional characteristics of dopamine (DA) release and uptake regulation are still not well-understood in zebrafish. In this study, we assessed D3 autoreceptor function in the telencephalon of whole zebrafish brains by measuring the electrically stimulated DA release ([DA]) and uptake at carbon fiber microelectrodes with fast-scan cyclic voltammetry.