[Experimental morphological study of the therapeutic effect of shark cartilage preparation in a model of infective allergic arthritis].

The therapeutic effect of a Polar shark cartilage preparation which is an enzymatic hydrolysate was studied in a rabbit model of infective allergic pseudotuberculous arthritis. Characterization of the chemical composition of the preparation designed by an original method is presented. Improvement of the general state of the affected joints and development of tissue immunomorphological responses were shown.

[Activity of proteolytic and nucleolytic enzymes from the gonades of hydrobionts].

The activity of nucleolytic and proteolytic enzymes in milt of nine kinds of fishes belonging to various families and of three kinds invertebrates is determined. There is carried out electrophoreses division of preparations DNA, received from milts by various methods; there are determined structure and molecular weights of oligonucleotides. The influence of activity tissue enzymes on a destruction degree of DNA is established at addition enzymes of exogenic origin.

[Inhibitory effect of antimicrobial preparation from lipids of marine fishes on tissue and microbial enzymes].

We obtained a new food preservative from marine fish lipids possessing pronounced activity in relation to bacteria and microscopic fungi. The effects of this preparation on enzymes of microorganisms and muscle tissue of marine hydrobionts were studied. In vitro the preparation irreversibly inhibited acid and alkaline proteases and proteolytic and lipolytic enzymes of microorganisms and reduced enzyme activity in fish muscle tissue.

[Effect of endogenous enzymes on composition of oligonucleotides isolated from gonads of hydrobionts].

Preparations of low-molecular-weight DNA were obtained from gonads of various hydrobiont species by alcohol precipitation technique. An enzymatic hydrolysis-based method for producing soluble low-molecular-weight DNA was developed. All the nucleic acid components of the preparations obtained were separated by electrophoresis, and their molecular weights were determined.

[Antiprotease and immunostimulating effect of peptide components isolated from cuticle of the chicken stomach].

Peptides with molecular weights of 0.8 to 16 kDa were isolated from the keratinous envelope of chicken muscular stomach cuticle. The peptide preparation had a immunostimulating activity whose qualitative and quantitative traits made it comparable with the activity of known drugs of similar composition.

[Enzymatic methods of preparation of protein hydrolysates by using proteolytic preparations of varying specificity].

The optimal conditions of enzymatic hydrolysis of proteins different composition and structure (protamin, casein, haemoglobin, fibrin, collagen) were established by using proteolytic enzymatic preparations with different specificity (pancreatin, pilrin, hepatopancreatin). The degree of hydrolysis, molecular and amino acid compositions of proteins were determined.

[Preparation and properties of trypsin from the pyloric caeca of Pacific Ocean salmon].

Trypsin from pyloric caeca of Pacific salmon was purified by affinity chromatography of the water extract on hexamethylenediamine-glycidylmethacrylate-cellulose. A protein band with a molecular weight of 22.5 kDa was found on SDS-electrophoresis in PAG.

[Trypsin-, chymotrypsin-like proteinases in fishes].

Recent data on the nature of trypsin-, chymotrypsin-like proteinases of fish are generalized. Localization and secretion of these enzymes in pyloric appendages of fish are considered in detail. Trypsin and chymotrypsin are in the state of proenzymes and transform into the active form by means of their own proteolytic factors.