Molecular epidemiology of Crimean-Congo hemorrhagic fever virus in Russia.

In this report, we present new data on the diversity and geographical distribution of genetic variants in Crimean-Congo hemorrhagic fever virus (CCHFV) in Russia. Partial S, M, and L segment sequences of the CCHFV genome were obtained from 500 serum samples from CCHF patients and 103 pools of ticks collected in the south of the European region of Russia in 2007-2017. The investigated viral strains belonged to the lineages Europe 1 (596 samples), Africa 3 (1 sample) and a new genetic lineage, Europe 3 (6 samples).

Phylogenetics of Bacillus anthracis isolates from Russia and bordering countries.

Anthrax is a concern for public health and veterinary medicine in Russia. The available phylogenetic data on isolates from Russia and neighboring CIS countries are clearly not enough to gain a better understanding of their position in the global phylogenetic population structure of this pathogen. In this study, we analyzed the genomes of 66 Bacillus anthracis strains, which were isolated between 1935 and 2019 from different sources in Russia, as well as in Ukraine, Azerbaijan, Georgia, Armenia and Moldova.

Yersinia pestis strains isolated in natural plague foci of Caucasus and Transcaucasia in the context of the global evolution of species.

Background: Plague is a highly dangerous vector-borne infectious disease that has left a significant mark on history of humankind. There are 13 natural plague foci in the Caucasus, located on the territory of the Russian Federation, Azerbaijan, Armenia and Georgia. We performed whole-genome sequencing of Y.

Molecular genotyping of 15 B. anthracis strains isolated in Eastern Siberia and Far East.

Bacillus anthracis is a pathogenic bacterium, which causes anthrax disease. The ability of this bacterium to form spores, which can be preserved in soil for decades and cause outbreaks later on, makes this pathogen a serious problem for veterinary and health services of many countries. Siberia is one of the most anthrax-influenced regions of Russia.

Diazapyrenes: interaction with nucleic acids and biological activity.

The review summarizes data on the practical aspects of the interaction of nucleic acids with diazapyrene derivatives. The information on biological activity is given and the probable mechanisms underlying the action of diazapyrenes are analyzed. It contains 119 references.

Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species.

Background: Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Previously, genotyping of Russian B.

Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia.

Background: Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B.

Global evolution and phylogeography of Brucella melitensis strains.

Background: Brucellosis is a bacterial zoonotic disease. Annually in the world more than 500,000 new cases of brucellosis in humans are registered. In this study, we propose an evolutionary model of the historical distribution of B.

[RETROSPECTIVE ANALYSIS OF BIOLOGICAL AND MOLECULAR-GENETIC PROPERTIES OF STRAINS - CAUSATIVE AGENTS OF CHOLERA - ISOLATED IN UKRAINE IN 1994 - 2011].

Aim: Retrospective analysis of biological and molecular-genetic properties of strains - cau- sative agents of cholera - isolated in the period of epidemics in Ukraine in 1994 - 2011.

Materials And Methods: Phenotypic and molecular-genetic properties of 5 strains of cholera vibrios, biovar El Tor isolated from cholera patients and 4 strains from the environmental samples were studied using traditional bacteriological and genetic methods. Detection of DNA for toxigenicity genes and genes characteristic for El Tor and classic biovar were carried out by PCR method using rea- gent kits <> and <> an experimental test system).

[New single nucleotide polymorphisms of androgen receptor gene (AR) in the Russian breed of Dzhalginsky Merino sheep].

This paper investigates the structure of androgen receptor gene (AR) in the Russian breed of Dzhalginsky Merino sheep. Polymorphisms of the gene were detected using NimbleGen sequencing technology (Roche, United States). Eight single nucleotide polymorphisms (SNPs) and two insertions were detected.

A NEW GENETIC VARIANT OF THE CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS ISOLATED IN CRIMEA.

This work represents the results of the genetic identification of the Crimean-Congo hemorrhagic fever virus (CCHF virus) strains isolated in the Crimean Federal District in conducting the epidemiological survey of the imported case of CCHF from Crimea in 2015. One sample of the serum from a patient and 61 pools (506 specimens) of ticks collected during the epizootiological survey of 6 administrative districts of the Crimean Federal District were tested using PCR for the presence of the CCHF virus RNA. RNA of the CCHF virus was detected in serum from a patient and 10 samples of ticks.

Associations Between Newly Discovered Polymorphisms of the CEBPD GENE LOCUS and Body Parameters in Sheep.

An understanding of what effects particular genes can have on body parameters in productive animals is particularly significant for the process of marker-assisted selection. The gene of transcriptional factor CCAAT/enhancer-binding protein delta (CEBPD gene) is involved in the process of growth in animals and is known to be a promising candidate for use as a genomic marker. The structure of the CEBPD gene locus was determined using NimbleGen sequencing technology (Roche, USA).

Comparative genomic analysis of two isolates of Vibrio cholerae O1 Ogawa El Tor isolated during outbreak in Mariupol in 2011.

Cholera is a water-borne, severe enteric infection essentially caused by toxigenic strains of Vibrio cholera O1 and O139 serogroups. An outbreak of cholera was registered during May-July 2011 in Mariupol, Ukraine, with 33 cholera cases and 25 carriers of cholera. Following this outbreak, the toxigenic strain of V.

[Comparative study of pharmacokinetics of ofloxacin in a free and niosomal forms in experiments on white mice when administered per os].

Aim: To study features of pharmacokinetics of ofloxacin as a part of anion PEGylated niosomes on a basis of sorbitan monostearate (Span 60) to experimental white mice per os.

Materials And Methods: Ofloxacin was entrapped in niosomes consisting of Span 60, cholesterol, PEG 4000 and dicetylphosphate. Sizes of niosomes estimated by means of probe microscopy.

[Morphological changes in male sexual glands in Kaluga regions contaminated with radionuclides].

41 testes of males who died after living 7-9 years in Kaluga region contaminated with radionuclides after the Chernobyl accident (1-5 and higher Ci/km2) were studied morphologically. Various alterations were found in 75.6% of the cases.

[Seven cases with angioid retinal strips].

Seven patients with angioid strips of the retina are described. In 5 patients angioid strips were concomitant with Darier's elastic pseudoxanthoma. In 3 the disease was hereditary (2 sisters and a daughter of one of them).

[Current characteristics of advanced and rapidly progressive forms of pulmonary tuberculosis].

Tuberculosis morbidity and mortality in the Moldova Republic has deteriorated since 1991. The percentage of advanced and rapidly progressive forms of the disease rose 2-3-fold. Most of the patients are 21-50-year-old unemployed men living in poor financial and social conditions.

[Effect of short-term hypoxia on body temperature and lactic acid in the blood of the mouse].

In mice, changes of body temperature and concentration of lactic acid in the blood were studied after three modes of barochamber hypoxia (altitude 8000 m) at the temperatures +10, +20 and +36 degrees C. The greatest drop of body temperature occurred at +10 degrees C, the least one at +36 degrees C. The lactic acid concentration was the greatest (up to 3.

[Urea synthesis in the myocardium].

Urea synthesis was studied in the isolated rat heart-perfused with ammonium chloride (10 mM), mixtures of ammonium chloride (10 mM) and 1-aspartic acid (10 mM), 1-ornithine (2.5 mM), 1-arginine (10mM), 1-glutamine (10 mM), 1-alanine (10 mM), 1-leucine (5 mM) and pyruvate (5mM). Ammonium chloride and 1-arginine are the most effective activators of urea synthesis, while 1-leucine and pyruvate produce an inhibitory action.