Laparoscopic and Robot-assisted Laparoscopic Reimplantation for Lower Ureter Pathology. A Multi-institutional Comparative Study in 1343 Patients.

Objective: To evaluate the outcomes of children with vesicoureteral reflux (VUR) and obstructive megaureter (OM) utilizing various laparoscopic and robot-assisted approaches.

Materials And Methods: Retrospective review of all pediatric laparoscopic and robot-assisted cases for lower ureter pathology was performed between 2016-2022 in 13 academic centers worldwide. Five surgical approaches were assessed: LEUR, LVCUR, LDECUR, RALUR, and RADECUR.

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay.

Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters.

Development and validation of a HPLC-MS/MS method for the analysis of fatty acids - in the form of FAME ammonium adducts - in human whole blood and erythrocytes to determine omega-3 index.

Recent scientific studies in the field of health and nutrition have unanimously affirmed the importance of consuming the omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), because of their cardioprotective properties. Fatty acid profiling in erythrocyte membranes allows the omega-3 index, which is a recognized indicator of the risk of developing cardiovascular disease, to be calculated. One consequence of the upward trend in healthy lifestyles and longevity is an increase in the number of studies into the omega-3 index, which requires a reliable method for the quantitative analysis of fatty acids.

Models of Ni- and Co-ion occupation in LiNiCoPOorthophosphate and its magnetic structure.

The neutron diffraction, magnetic and heat capacity measurements have been carried out to study the polycrystalline sample LiNiCoPOprepared by the glycerol-nitrate synthesis method. Models of Ni- and Co-ion occupation the 4octahedral position in a crystal structure LiNiCoPOare calculated for a paramagnetic state. The best model is the Ni- and Co-ions occupy the 4site inpatent space-group in sequence Ni-Co-Ni-Co or Co-Ni-Co-Ni.

Estimating population-level of alcohol, tobacco and morphine use in a small Russian region using wastewater-based epidemiology.

Introduction: Alcohol, tobacco and illicit drug use combined are the largest modifiable health risk factors. Wastewater-based epidemiology (WBE) is a complementary approach for monitoring substance use in the population. In this study we applied WBE technique to a community in the Moscow region to estimate population-level consumption of alcohol, tobacco and morphine.

A novel simple and sensitive approach for determination of 1,1-dimethylhydrazine in aqueous samples by high performance liquid chromatography with ultraviolet and tandem mass spectrometric detection after derivatization with unsubstituted aromatic aldehydes.

In this work, simple, rapid and highly sensitive method of hazardous chemical 1,1-dimethylhydrazine (unsymmetrical dimethylhydrazine, UDMH) determination based on pre-column derivatization with unsubstituted aromatic aldehydes and reversed-phase high performance liquid chromatography-ultraviolet-tandem mass spectrometry (RP HPLC-UV-MS/MS) has been developed. Along with benzaldehyde, commercially available aromatic aldehydes, namely: 2-naphthaldehyde, 2-pyridinecarboxaldehyde, and 2-quinolinecarboxaldehyde, were used as derivatizing reagents in the analysis of hydrazines for the first time. The reactions were studied in a wide pH range by varying reaction time and other conditions.

Risk factors for obstructive complications after endoscopic correction of vesico-ureteral reflux using polyacrylate polyalcohol copolymer.

Use of polyacrylate-polyalcohol copolymer (PPC) after endoscopic correction (EC) of vesico-ureteral reflux (VUR) is highly effective but is associated with a higher risk of obstructive complications (OC) compared with other implants. We undertook a STROBE compliant retrospective investigation and studied the OC risk factors to increase the practical safety of PPC.Overall, 798 patients (464 [58.

Destruction of the bronchial epithelium in patients with severe asthma according to different patterns of inflammation and cold airway hyperresponsiveness.

Aim: The aim of the research was to study the state of the bronchial mucosa epi-thelium in relation to the severity of clinical manifestations in severe uncon-trolled asthma depending on the pattern of inflammation and the presence of cold airway hyperresponsiveness.

Materials And Methods: In 48 patients with severe uncontrolled asthma, there were assessed asthma symptoms, clinical signs of cold airway hyperre-sponsiveness, and lung function; the samples of slides were analyzed in the cytological examination of the sputum; the degree of damage to epithelial cells and granulocytes was estimated using the total cell destruction index (CDI).

Results: According to the analysis of sputum cytograms, the patients were divided into two groups: group I (22 patients) included persons with eosin-ophilic inflammation pattern (31.

High-complexity regions in mammalian genomes are enriched for developmental genes.

Motivation: Unique sequence regions are associated with genetic function in vertebrate genomes. However, measuring uniqueness, or absence of long repeats, along a genome is conceptually and computationally difficult. Here we use a variant of the Lempel-Ziv complexity, the match complexity, Cm, and augment it by deriving its null distribution for random sequences.

Monolithic thin-layer chromatography plates with covalently bonded matrix for hyphenation with matrix-assisted laser desorption/ionization.

Monolithic layers prepared by the copolymerization of ethylene dimethacrylate and glycidyl methacrylate and deposited onto the surface of glass and silicon plates were investigated as thin-layer chromatography separation media in hyphenated thin-layer chromatography with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. Varying composition of the polymerization mixture and polymerization conditions layers of different porosities and MALDI-MS compatibility were synthesized. Compatibility with MALDI-MS was tested using polyethylene glycol and it was demonstrated that layers prepared without glycidyl methacrylate are not compatible with MALDI-MS and do not allow obtaining any mass spectra.

Recent advances in the preparation of adsorbent layers for thin-layer chromatography combined with matrix-assisted laser desorption/ionization mass-spectrometric detection.

This review is focused on planar chromatography hyphenated with mass-spectrometric detection for analysis of low-molecular-mass solutes. Various kinds of hyphenations are discussed with attention paid to the preparation of thin layer plates suited both for the mass-spectrometric detection of the resolved solutes direct from thin-layer plates and for indirect mass-spectrometric detection of the resolved solutes, performed by scraping, extracting, purifying, and concentrating the analyte from the thin-layer chromatography plate. Plates with monolithic layers are relatively new for thin-layer chromatography but they can successfully be combined with mass-spectrometric technique in a pursuit of comprehensive local sample composition information.

[Experience of 157 vesikoscopic operations in children].

Aim: Recent advances in the field of minimally invasive surgical technologies in children and adolescents have led to the development of vesicoscopic (transvesical, pneumoscopic) access (VA). Current limitations in using VA emphasize the need for further studies investigating surgical options for the management of various pathological conditions of the bladder and ureterovesical junction, the features of surgical techniques and the course of the early postoperative period when used in pediatric urological practice.

Materials And Methods: From 2013 to 2017, 157 patients (79 girls and 78 boys) aged between 2 months and 18 years (mean age 4.

[Laparoscopic heminephrureterectomy for duplex kidney in children].

Introduction: Duplication of the upper urinary tract is one of the most common congenital urological anomalies. In patients with critically decreased or lost function of one of the renal segments, heminephrureterectomy is usually the treatment of choice. Until recently, this was an open surgery; in cases of complete removal of the ureter, an additional incision in the iliac region was required.

A novel multi-purpose enzymatic system and procedures for the rapid fluorescent determination of flavonoids in herbal pharmaceuticals and plant materials.

The paper presents a novel multi-purpose enzymatic system and procedures for fluorescent determination of several flavonoids in herbal pharmaceuticals and plant materials after their enzyme-catalyzed oxidation by hydrogen peroxide and further derivatization with meso-1,2-diphenylethylenediamine. This system may be used for rapid (15-30min/20 samples) simultaneous screening of samples containing a certain flavonoid in a standard microplate, or as a HPLC detection system for analyzing plant extracts with uncertain composition. In the first case, this indicator system provides sensitive and reproducible microplate determination of quercetin, epicatechin, caffeic acid, and taxifolin in the ranges 0.

Inflammatory Pattern of the Bronchial Mucosa in Patients with Asthma with Airway Hyperresponsiveness to Hypoosmotic Stimulus.

Positive reaction of the bronchi to distilled water inhalation in asthmatics is associated with significant stimulation of the respiratory epithelium desquamation against the background of increased content of eosinophilic and neutrophilic leukocytes in induced sputum, predomination of eosinophil and neutrophil cytolysis, and lower activity of myeloperoxidase in leukocyte granules (in comparison with the parameter in patients with a negative response to bronchostimulation). Enhanced cytolysis and destruction of leukocytes and high myeloperoxidase concentration in the extracellular space are essential for the development of bronchial hyperresponsiveness to hypoosmotic stimulus in asthma.

Polarization in RMnO3 multiferroics.

Some comments on the review by Sim et al. [(2016). Acta Cryst.

[POSSIBILITIES FOR THE USE OF THE ANTI-LEUKOTRIENE DRUG MONTELUKAST IN COMBINED THERAPY OF PATIENTS WITH BRONCHIAL ASTHMA AND COLD-INDUCED BRONCHIAL HYPERACTIVITY].

Aim: To study effectiveness of the use of the anti-leukotriene drug montelukast in combination with inhalation glucocorticoid and long-acting beta-agonist in patients with bronchial asthma (BA) and cold-induced bronchial hyperactivity (CBHA) with a view to optimizing control of the disease.

Materials And Methods: We carried out an open comparative prospective study of patients with persistent BA in a cold season under conditions of real clinical practice. The patients were divided into 2 groups.

[Possibility of achieving and maintaining asthma control in patients with bronchial cold hyperreactivity].

Aim: To evaluate the clinical efficiency of tactics to widen the scope of monotherapy with inhaled glucocorticosteroids (IGCS) in asthmatic patients with bronchial cold hyperreactivity (BCHR) during winter to achieve control of the disease in real clinical practice.

Subjects And Methods: An open-label longitudinal study was conducted in a cold period in 106 asthmatics divided into 2 groups: 1) those with BCHR and 2) those with unchanged bronchial reactivity to a cold stimulus. The study involved monitoring the symptoms by the asthma control test, peak expiratory flow rate (PEFR), and spirometry results before and after cold bronchoprovocation testing; assessment of the pattern of bronchial inflammation from the ratios of induced sputum (IS) cell populations; and estimation of the number of asthma exacerbations and emergency care recourses.

Self-assembled nanocrystals discovered in Chelyabinsk meteorite.

Current interest in nanomaterials is focused mostly on artificial materials fabricated for various applications. However, naturally occurring nanocrystal arrays, like those recently found in the meteorite that fell near Chelyabinsk in Russia on 15 February, 2013, can provide some new insights into the nature of nanomaterials, including the conditions for their natural occurrence. Here we report the results of our spectroscopic investigation of a fragment of the Chelyabinsk meteorite.

Preparation and characterisation of anion exchangers with dihydroxy-containing alkyl substitutes in the quaternary ammonium functional groups.

Novel poly(styrene-divinylbenzene) (PS-DVB) based anion exchangers having one and two (2,3-dihydroxypropyl) substitutes in the quaternary ammonium functional groups are prepared and characterised by ion chromatography (IC). The introduction of bulky and hydrophilic substitutes to the anion-exchange groups allows the elimination of non-ionic interactions between the polarisable anions and the aromatic rings from the matrix and the improvement of separation selectivity as compared with the traditional trimethylammonium (TMA) functionalised ion exchangers. The synthesis of the ion exchangers includes acylation of PS-DVB particles with acetic anhydride followed by reductive amination either with methylamine hydrochloride or dimethylamine and further alkylation with oxiranes under varied conditions.

Effect of copper oxide on the structure of nano-sized Ni(x)Zn(y)Fe2O4 powders prepared by using self-propagating high temperature synthesis.

Nano-sized Cu(x)Ni(x)Zn(1-x-y)Fe2O4 ferrites were prepared by using self-propagating high-temperature synthesis (SHS) and mechanical ball milling. As oxygen pressure increases and copper content decreases in the initial composition, average combustion temperature and combustion velocity increases in the ranges of 947 to 1150 degrees C and 4.2 to 6.

Effect of monomer mixture composition on structure and chromatographic properties of poly(divinylbenzene-co-ethylvinylbenzene-co-2-hydroxyethyl methacrylate) monolithic rod columns for separation of small molecules.

Porous poly(divinylbenzene-co-ethylvinylbenzene-co-2-hydroxyethyl methacrylate) monoliths were synthesized via thermally initiated free-radical polymerization in confines of surface-vinylized glass columns (150 mm × 3 mm i.d.) and applied to the reversed-phase separation of low-molecular-weight aromatic compounds.

[Pleiotropic effects of small dozes simvastatin in patients of senile age with chronic heart failure].

The pleiotropic effects of simvastatin in a daily doze of 20 mg in persons of senile age with chronic heart failure were studied in the submitted work. The practical opportunity to reduce the risk of acute vascular events, improvement of vascular endothelium function and relative safety of application of the preparation in the specified dosage at persons of senile age is shown.

Determination of underivatized glucosamine in human plasma by high-performance liquid chromatography with electrochemical detection: application to pharmacokinetic study.

A simple, rapid and sensitive high-performance liquid chromatography method with electrochemical detection was developed for the determination of glucosamine in human plasma. Plasma samples were analyzed after a simple two-step procedure of protein precipitation with subsequent dilution. The chromatographic separation was performed on a Carbopack column (3 mm x 150 mm) with a mobile phase consisting of water and 200 mM sodium hydroxide.