A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application.

Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features of this technique. Constructional methodological challenges to PPEC are obstacles to its development and application in laboratory practice.

Preliminary results for 2-D separation with high-performance thin-layer chromatography and pressurized planar electrochromatography.

Preliminary results of 2-D separation of test dye mixture using high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) are demonstrated. The advantage of 2-D HPTLC/PPEC separation is based on different separation selectivities obtained in both HPTLC and PPEC systems. HPTLC RP18 W plates of 5x20 cm from Merck were used in the investigations.

Progress in planar electrochromatography.

Developments in planar electrochromatography in open (PEC) and closed (PPEC) systems are reviewed. The discussion focuses on progress in chamber construction for planar electrochromatography, separating system performance, equilibration of the PPEC process, separation time and selectivity, and the general advantages, disadvantages and prospects of this separation mode.

Influence of sample application mode on performance of pressurized planar electrochromatography in completely closed system.

Three modes of sample application on the chromatographic plate are applied at present investigations of pressurized planar electrochromatography (PPEC) systems taking into special attention their influence on performance of the separating system. These modes are as follows: application of the sample solution directly on the chromatographic plate with microsyringe, deposition of sample solution on scrap of adsorbent layer followed by location oft this scrap on the chromatographic plate, application of the sample solution with commercially available aerosol applicator. These modes were combined with prewetting procedures of the chromatographic plates which lead to an accomplishment of equilibration of the stationary phase-mobile phase system.

Apparatus for pressurized planar electrochromatography in a completely closed system.

Pressurized planar electrochromatography (PPEC) is the mode which offers much higher separation efficiency in comparison to conventional planar chromatography, including both higher performance and much higher speed of separation. In this paper, we present a new device for performing PPEC in which the whole area of the chromatographic plate is pressurized. Both electrodes (anode and cathode) are washed with the mobile phase during the experiment, which prevents gas bubbles from collection in the region of the electrodes.