Increase in cardiac output and PEEP as mechanism of pulmonary optimization.

The influence of cardiac output (CO) and PEEP on pulmonary shunt (Qs/Qt) has been the subjects of considerable investigation but findings are controversial. The role of CO and PEEP on 19 isolated rabbit lung preparations perfused with hypoxic mixture (6% CO2, 10% O2, and 84% N2), which resulted in a constant oxygen venous pressure (64 +/- 5.6 mmHg) has been studied.

Inducible nitric oxide synthase activity in myocardium after myocardial infarction in rabbit.

The activity of nitric oxide synthase (NOS) in infarcted and noninfarcted rabbit myocardium was determined. NOS activity, as measured by conversion of [14C]arginine to [14C]citrulline, was significantly higher in the infarcted area of myocardium (22.7 +/- 3.

[The estimation of LHRH receptors in the tissue of human leiomyoma, myometrium and endometrium].

Uterine leiomyoma is the most common female benign pelvic tumor, affecting 20-25% of women during their reproductive years. There is strong clinical evidence that these tumors are estrogen-dependent for their growth, as also supported by their clear regression after the menopause. Although large clinical trials have not yet been reported, according to the estrogen dependency of uterine fibroids, LHRH agonists have been shown to be effective in the treatment of these conditions because they produce a condition of temporary hypoestrogenism secondary to the specific hypogonadotrophinism.

Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium.

Infarcted areas of rabbit myocardium show relatively higher inducible nitric oxide synthase activity, measured by the conversion of L-[14C]arginine to L-[14C]citrulline. The principal finding in this study is that dexamethasone (2 mg/kg) prevents the induction of inducible nitric oxide synthase in heart muscle when given before, or even 3 hr after coronary artery ligation. Additionally cyclic GMP levels remain unchanged following treatment with dexamethasone.

Effect of amphiphiles on nitric oxide synthase in endothelial cells.

Amphiphiles are known to modulate the activity of ATPase, phospholipase A2, adenylate and guanylate cyclase amongst others and relax vascular smooth muscle. The effect of two amphiphiles, lysophosphatidylcholine (LPC) and digitonin on the activity of nitric oxide synthase (NOS), as measured by conversion of radiolabeled L-arginine to L-citrulline, has been studied. Neither digitonin (0.

Extracellular matrix-induced stimulation of a CD4-8- thymic T cell line.

PBK101 was a thymic T cell line of AKR/J origin that expressed Thy 1, CD44, IL-2R, and VLA-4 antigens but not CD4, CD8, or the TCR/CD3 complex. PBK101 was initially isolated growing together with adherent thymic stromal cells. The proliferation of PBK101 ceased when it was cultured in the absence of stromal cells and its proliferation was stimulated when stromal cells were added back to the culture.

[Colonic polyps. Experience at a clinical center in Caracas].

After six years experience in the anatomopathological study and having checked 33.452 histological cases 772 corresponded to colon tumoral pathology. It was then decided to proceed with the epidemiological analysis of the polyps, it's anatomical distribution according to sex and age, it's histolopathological characteristics and the possible relationship with malignant transformation.

[Kerion of the vulva. Report of a case and review of the literature].

We report a case of a 43 year old woman who developed an extensive vulvar kerion caused by Trichophyton menthagrophytes that was initially mistaken for a bacterial infection. The peculiarity of this case lies in the fact that the kerion is rarely located in the vulva and it has not yet been described in the literature. On the contrary, cases have been found in the scalp, beard, eye-brow, etc.

[Adenosquamous carcinoma of the colon. Report of 2 cases and review of the literature].

The adenosquamous carcinoma of colon is an uncommon neoplasia, it only represents 0.1% of all carcinomas of colon. We report two new cases, that are added to the 39 reported in the medical literature.

The mitochondrial permeability transition pore may comprise VDAC molecules. II. The electrophysiological properties of VDAC are compatible with those of the mitochondrial megachannel.

The electrophysiological properties of isolated mitochondrial porin (VDAC), reconstituted in planar bilayers or proteoliposomes, resemble those of the mitochondrial megachannel believed to be the permeability transition pore. In particular, a correspondence was found with regard to the voltage dependence: VDAC was driven to closed states by potentials of either sign, but the effect was not symmetrical; voltages negative in the compartment to which VDAC was added were more effective. The results are consistent with the hypothesis that the PTP may consist of two cooperating VDAC channels, plus presumably an adenine nucleotide carrier dimer and a third component known to be part of the mitochondrial benzodiazepine receptor.

Location of the dicyclohexylcarbodiimide-reactive glutamate residue in the bovine heart mitochondrial porin.

The mitochondrial porin or VDAC (Voltage-Dependent Anion Channel), the pore-forming structure responsible for the high permeability of the outer mitochondrial membrane, was found to be one of only three mitochondrial proteins bound by [14C]dicyclohexylcarbodiimide (DCCD) at low dosages (1.5 nmol/mg of mitochondrial porin) (De Pinto, V., Tommasino, M.

Purification and Characterization of Porin from Corn (Zea mays L.) Mitochondria.

Mitochondrial porin from corn (Zea mays L. B 73) shoots was solubilized with lauryl(dimethyl)-amine oxide and purified by chromatography on a hydroxyapatite:celite column. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified protein had an apparent molecular mass of 35 kD.

Simplified ondansetron regimens for antiemetic prophylaxis in cisplatin-based chemotherapy of ovarian cancer.

In past years prevention of emesis and nausea induced by antiblastics has been based on extremely heterogeneous protocols with little efficacy. However, by the use of selective antagonists of serotonin S3 receptors there has been a remarkable improvement in the control of side effects. Among these antagonists ondansetron has been till now the most studied.

[Low-grade non-Hodgkin's lymphomas. Study of 73 cases].

Purpose: To analyse the clinico-biologic features at diagnosis and the response to therapy and survival of a group of patients with low-grade non-Hodgkin's lymphoma (NHL).

Material And Methods: The study comprises 73 NHL patients diagnosed between 1974 and 1989 in the Covadonga Hospital and classified as low-grade in accordance with the international Working Formulation. The first-line treatment regimens used were cyclophosphamide-vincristine-prednisone (CVP), chlorambucil-prednisone (CBL-PRED), radiotherapy, and other combinations.

[Oral health status in people 60 years old or older in the municipality of São Paulo (Brazil)].

The oral health status of people aged 60 years or over in S. Paulo in 1989 is discussed. According to data relating to dental caries, periodontal diseases, need and use of prosthesis and prevalence of hard and soft oral tissue pathologies, this study concludes that elder people present a very bad oral health status.

[Uncomplicated pregnancy in patients with unrecognized primary biliary cirrhosis].

A 32 yr-old woman suffering from an unsuspected primary biliary cirrhosis (PBC), completed an uneventual pregnancy. She only experienced diffuse pruritus and subicterus, which misled to an antepartum diagnosis of intrahepatic cholestasis of pregnancy. The most remarkable points of the reported case are: (a) the younger age of onset of PBC; (b) the paucisymptomatic course of PBC; (c) the rarity of PBC-associated pregnancy (only 15 previous instances in 12 patients, from a review of the literature); (d) the uncomplicated course of pregnancy for both the mother and the fetus, which is the exception rather than the rule, in such a condition.

Transmembrane arrangement of mitochondrial porin or voltage-dependent anion channel (VDAC).

Porin or voltage-dependent anion-selective channel (VDAC) is the main protein responsible for the high permeability of the outer mitochondrial membrane. The mitochondrial porin is mainly composed of sided beta-strands, in analogy with bacterial porin, whose structure has been resolved at 1.8 A resolution.

11-Oxycorticosteroid-dependent antibody in the serum of a patient with bladder neoplasm.

An IgM 11-oxycorticosteroid-dependent antibody was identified in the serum of a patient with bladder malignancy, which at 4-37 degrees C reacted with all the 11-oxycorticosteroids tested but not with desoxycorticosterone, testosterone, nortestosterone and progesterone. The resulting drug-antidrug antibody complex combines nonspecifically with red blood cells causing agglutination. This reactivity is enhanced both by acid pH and by high drug solution concentrations as well as by ficin test.

Filters for the reduction of baseline wander and muscle artifact in the ECG.

Contamination of the electrocardiographic (ECG) signal by extraneous electrical potentials is a significant problem in exercise electrocardiography. Baseline wander and muscle artifact are particularly troublesome sources of interference. This paper describes two digital filters that were constructed and found effective in reducing these two types of signal contamination.

Characterization of SH groups in porin of bovine heart mitochondria. Porin cysteines are localized in the channel walls.

Porin from bovine heart mitochondria contains probably two cysteines (Cys126 and Cys230 in human porin, Kayser, H., Kratzin, H. D.

[Transfusion evaluation measured by a retrospective audit].

From a sample of 759 transfusions requests (2123 Units) for 392 patients, evaluated by retrospective audits the incidence of inappropriate transfusion at our Hospital was found to be 25.8% of request, affecting 23.3% of units and 39.