LRRC56 deletion causes primary ciliary dyskinesia in mice characterized by dynein arms defects.

Leucine Rich Repeat Containing protein 56 (LRRC56), also known as DNAAF12, is a member of the LRRC superfamily, whose dysfunction is associated with mucociliary clearance and laterality defects in humans. Here, we generated LRRC56-knockout mice using the CRISPR/Cas9 nuclease system to specifically target exons 4-5 of the LRRC56 gene. We observed that homozygous LRRC56 gene deletion is definitely deleterious, as 27.

Bactericidal and anti-quorum sensing activity of repurposing drug Visomitin against .

With the growing antibiotic resistance in , it is imperative to develop innovative therapeutic strategies against new targets to reduce selective survival pressures and incidence of resistance. In , interbacterial communication relies on a quorum sensing system that regulates gene expression and physiological activities. Here, we identified that Visomitin, an antioxidant small molecule, exhibited bactericidal efficacy against methicillin-resistant and its high tolerance phenotypes like intracellular bacteria and persister cells without inducing resistance.

Insights into the antimicrobial effects of tafenoquine against Enterococcus and its biofilms.

Aim: The purpose of this study is to assess the in vitro antimicrobial and anti-biofilm effects of the anti-protozoal agent tafenoquine (TAF) on Enterococcus and elucidate its underlying mode of action.

Methods And Results: The present work investigated the susceptibility of TAF on 3 type strains and 11 clinical isolates of enterococci. The results indicated that TAF exhibited powerful antimicrobial activity against both of Enterococcus faecalis and Enterococcus faecium with minimum inhibitory and bactericidal concentrations ranging from 8 to 16 µg ml-1.

Simeprevir restores the anti-Staphylococcus activity of polymyxins.

Methicillin-resistant Staphylococcus aureus (MRSA) infection poses a severe threat to global public health due to its high mortality. Currently, polymyxins are mainly used for the treatment of Gram-negative bacterial-related infection, while exhibiting limited antibacterial activities against Staphylococcus aureus (S. aureus).

Inhibitory effects of simeprevir on and itsbiofilm in vitro.

Objectives: () is a Gram-positive opportunistic pathogen that often causes hospital infections. With the abuse of antibiotics, the resistance of gradually increases, and drug repurposing has become a research hotspot in the treating of refractory drug-resistant bacterial infections. This study aims to study the antimicrobial and antibiofilm effects of simeprevir, an antiviral hepatitis drug, on in vitro.