Multivariate modular metabolic engineering and medium optimization for vitamin B production by .

Vitamin B is a complex compound synthesized by microorganisms. The industrial production of vitamin B relies on specific microbial fermentation processes. has been utilized as a host for the biosynthesis of vitamin B, incorporating approximately 30 heterologous genes.

A synthetic cell-free 36-enzyme reaction system for vitamin B production.

Adenosylcobalamin (AdoCbl), a biologically active form of vitamin B (coenzyme B), is one of the most complex metal-containing natural compounds and an essential vitamin for animals. However, AdoCbl can only be de novo synthesized by prokaryotes, and its industrial manufacturing to date was limited to bacterial fermentation. Here, we report a method for the synthesis of AdoCbl based on a cell-free reaction system performing a cascade of catalytic reactions from 5-aminolevulinic acid (5-ALA), an inexpensive compound.

Optimization of hydrogenobyrinic acid biosynthesis in Escherichia coli using multi-level metabolic engineering strategies.

Background: Hydrogenobyrinic acid is a key intermediate of the de-novo aerobic biosynthesis pathway of vitamin B. The introduction of a heterologous de novo vitamin B biosynthesis pathway in Escherichia coli offers an alternative approach for its production. Although E.

Metabolic engineering and optimization of the fermentation medium for vitamin B production in Escherichia coli.

Vitamin B is a crucial fine chemical that is widely used in the pharmaceutical, food and chemical industries, and its production solely dependents on microbial fermentation. We previously constructed an artificial vitamin B biosynthesis pathway in Escherichia coli, but the yield of the engineered strains was low. Here, we removed metabolic bottlenecks of the vitamin B biosynthesis pathway in engineered E.