[Forward genetic screening for zebrafish mutants defective in myelopoiesis].

Objective: To identify zebrafish mutants with myelopoiesis defects by ENU mutagenesis and large-scale forward genetic screening.

Methods: Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in the spermatogonial cells to generate the founders, which were outcrossed with AB to raise F1 fish. The F1 fish from different founders were mated to generate the F2 families.