[SRY gene-testing in the diagnosis of disorders of sex development among children].

Objective: To investigate the value of direct sequencing of sex-determining region Y (SRY) gene, as well as peripheral blood karyotype analysis, in the diagnosis of disorders of sex development (DSD) among children and adolescents with ambiguous genitalia.

Methods: The karyotypes of 20 children and adolescents with ambiguous genitalia were determined by conventional G-banding analysis. PCR amplification was used to detect SRY gene in these patients, and direct sequencing was used to judge whether there was SRY gene mutation.

[Study on the intercellular molecule-1 polymorphisms in an Chinese population with myocardial infarction].

Objective: To investigate the association between intercellular adhesion molecule-1 (ICAM-1) gene + 12959G/A and + 13848A/G polymorphism and myocardial infarction (MI).

Methods: Polymerase chain reaction-sequence specific primers (PCR-SSP) technology and PCR-restriction fragment length polymorphism(PCR-RFLP) were used for the detection of ICAM-1 genotypes in 165 patients with MI(including acute MI and old MI) and 199 healthy controls. Plasma lipid levels and hyper sensitive C reactive protein (quantitive ELISA method was used) levels were measured in all subjects.

[Tumor necrosis factor-alpha gene polymorphisms and risk of coronary heart disease].

Tumor necrosis factor-alpha(TNF-alpha) is a cytokine that has multiple functions. It could be involved in cardiovascular pathophysiology. Given this possibility, we measured serum TNF-alpha level by ELISA and use PCR-RFLP methods to examine the distribution of TNF-alpha locus gene polymorphism at -857 and -863 sites in coronary heart disease(CHD) patients and matched controls.