Widely Untargeted Metabolomics Profiling Combined with Transcriptome Analysis Provides New Insight into Amino Acid Biosynthesis at Different Developmental Stages of Hu (Chinese Raspberry).

The composition and profile of amino acids in Hu serve as critical indicators of its nutritional quality. A comprehensive understanding of the amino acid metabolism within is instrumental in the formulation and innovation of functional foods derived from this species. Utilizing advanced techniques such as wide-ranging untargeted metabolomics, transcriptome analysis, interaction network mapping, heat map analysis, and quantitative real-time PCR, we conducted a comprehensive assessment of the quality attributes across four distinct developmental stages of Our meticulous analysis uncovered a rich tapestry of 76 distinct amino acids and their derivatives within the developmental stages of .

rare variant augments aortic growth and induces cardiac hypertrophy and heart failure with pressure overload.

Smooth muscle cell-specific myosin heavy chain, encoded by , is selectively expressed in smooth muscle cells (s). Pathogenic variants in predispose to a number of disorders, including heritable thoracic aortic disease associated with patent ductus arteriosus, visceral myopathy, and megacystis-microcolon-intestinal hypoperistalsis syndrome. Rare variants of uncertain significance occur throughout the gene, including p.

Analysis of molecular epidemiological characteristics and antimicrobial susceptibility of vancomycin-resistant and linezolid-resistant Enterococcus in China.

Background: This study investigates the distribution and characteristics of linezolid and vancomycin susceptibilities among Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) and explores the underlying resistance mechanisms.

[Analysis of Risk Factors for Recurrent Pregnancy Loss in Patients Undergoing Fertilization-Embryo Transfer].

Objective: Recurrent pregnancy loss (RPL) presents a formidable challenge for individuals undergoing fertilization-embryo transfer (IVF-ET), forming both a clinical dilemma and a focal point for scientific inquiry. This study endeavors to investigate the intricate interplay between clinical features, such as age, body mass index (BMI), and waist-to-hip ratio (WHR), and routine laboratory parameters, including sex hormones, blood composition, liver and thyroid functions, thyroid antibodies, and coagulation indicators, in RPL patients undergoing IVF-ET. By meticulously analyzing these variables, we aim to uncover the latent risk factors predisposing individuals to RPL.

Live birth rate per fresh embryo transfer and cumulative live birth rate in patients with PCOS under the POSEIDON classification: a retrospective study.

Background: Ovarian stimulation (OS) for fertilization (IVF)/intracytoplasmic sperm injection (ICSI) in women with PCOS often results in multiple follicular development, yet some individuals experience poor or suboptimal responses. Limited data exist regarding the impact of poor/suboptimal ovarian response on pregnancy outcomes in women with PCOS.

Objectives: The aim of this study was to evaluate whether the live birth rate (LBR) per fresh embryo transfer and cumulative live birth rate (CLBR) per aspiration cycle differ in women with PCOS defined by the Patient-Oriented Strategy Encompassing IndividualizeD Oocyte Number (POSEIDON) criteria.

GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells.

In Brief: Ovarian aging results in reactive oxygen species accumulation and mitochondrial deterioration. During the aging process, GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells.

Abstract: Ovarian aging critically influences reproductive potential, with a marked decrease in oocyte quality and quantity and an increase in oxidative stress and mitochondrial dysfunction.

The chemical stoichiometry characteristics of plant-soil carbon and nitrogen in subtropical Pinus massoniana natural forests.

Ecological stoichiometry is essential for understanding changes in ecosystem structure and nutrient cycling in forest ecosystems. However, the stoichiometric characteristics of carbon (C) and nitrogen (N) in different organs or layers, such as leaves, branches, trunks, roots, understory vegetation, litter, and soil within a forest ecosystem, have remained poorly understood. In this study, four age groups of Pinus massoniana natural forest including young, middle-aged, near-mature, and mature were selected as research subjects to illustrate the C and N stoichiometry interactions among different layers and organs in the forest ecosystem.

Degradation of Ciprofloxacin in Water by Magnetic-Graphene-Oxide-Activated Peroxymonosulfate.

Antibiotics are extensively applied in the pharmaceutical industry, while posing a tremendous hazard to the ecosystem and human health. In this study, the degradation performance of ciprofloxacin (CIP), one of the typical contaminants of antibiotics, in an oxidation system of peroxymonosulfate (PMS) activated by magnetic graphene oxide (MGO) was investigated. The effects of the MGO dosage, PMS concentration and pH on the degradation of CIP were evaluated, and under the optimal treatment conditions, the CIP degradation rate was up to 96.

Spatiotemporal variation and driving factor of vegetation coverage from 2000 to 2020 in southern Jiangxi Province, China.

Vegetation plays a critical role in the water and carbon cycling and energy flow, serving as an indicator for regulating land carbon balance and reflecting climate change and human activities. We analyzed the spatiotemporal variations of normalized difference vegetation index (NDVI) during the growing season in southern Jiangxi from 2000 to 2020, using statistical methods, including the Mann-Kendall test, Theil-Sen Median analysis, Hurst index, and coefficient of variation. We employed the geodetector model to comprehensively assess the impacts of climate, topography, soil and human factors on spatial differentiation of vegetation NDVI.

Endocrine profiles and cycle characteristics of infertile 17α-hydroxylase/17,20-lyase Deficiency Patients undergoing assisted Reproduction Treatment: a retrospective cohort study.

Background: 17α-hydroxylase/17,20-lyase deficiency (17-OHD) is a rare form of congenital adrenal hyperplasia caused by CYP17A1 gene variants. Female patients with 17-OHD demonstrate a broad clinical spectrum, including oligomenorrhea or amenorrhea and infertility, often as the sole manifestation. However, no spontaneous pregnancies in affected women have been reported.

Histone methyltransferase SETDB1 promotes osteogenic differentiation in osteoporosis by activating OTX2-mediated BMP-Smad and Wnt/β-catenin pathways.

Osteogenic differentiation plays important roles in the pathogenesis of osteoporosis. In this study, we explored the regulatory mechanism of histone methyltransferase SET domain bifurcated 1 (SETDB1) underlying the osteogenic differentiation in osteoporosis. The common osteoporosis-related genes were retrieved from the GeneCards, CTD, and Phenolyzer databases.

Recent strategies for electrochemical sensing detection of miRNAs in lung cancer.

MicroRNAs (miRNAs) associated with lung cancer are diversifying. MiR-21, Let-7, and miR-141 are common diagnostic targets. Some new lung cancer miRNAs, such as miR-25, miR-145, and miR-126, have received increasing attention.

Cr-MOF-Based Electrochemical Sensor for the Detection of P-Nitrophenol.

Cr-MOF nanoparticles were synthesized by a simple hydrothermal method, and their morphology and structure were characterized by SEM, TEM, and XRD techniques. The Cr-MOF modified glassy carbon electrode (Cr-MOF/GCE) was well constructed and served as an efficient electrochemical sensor for the detection of p-nitrophenol (p-NP). It was found that the Cr-MOF nanoparticles had significant electrocatalytic activity toward the reduction of p-NP.

[Identification of forest vegetation types in southern China based on spatio-temporal fusion of GF-1 WFV and MODIS data].

It is difficult to obtain long time series of high spatial resolution remote sensing images in southern China because of the complex terrain and frequent cloudy and rainy weather. In contrast, the spatio-temporal fusion can sychonorously obtain remote sensing data with high spatial-temporal resolution, which is beneficial to extract forest vegetation type information. With Xingguo County of Jiangxi Province as the study area, we fused the Landsat8 OLI and GF-1 WFV images with high spatial resolution with high temporal resolution of MODIS09 A1 image on the basis of enhanced spatial and temporal adaptive reflectance fusion model (ESTARFM), reconstructed the time series data of ESTARFM_Landsat8 EVI and ESTARFM_GF-1 EVI with 8 d step of enhanced vegetation index (EVI), obtained the phenology (PH) characteristics, and identified the forest vegetation types by using random forest classification model.

The Degree of Menstrual Disturbance Is Associated With the Severity of Insulin Resistance in PCOS.

Objective: Insulin resistance (IR) is an important determinant of the phenotype and morbidity of the polycystic ovary syndrome (PCOS). In this study, we aimed to figure out the association between the degree of menstrual disturbance and the severity of IR in women with PCOS.

Design: It is a cross-sectional study conducted in an academic tertiary setting.

Effect of Hcp Iron Ion Regulation on the Interaction Between With Human Pulmonary Alveolar Epithelial Cells and Biofilm Formation.

is a type of bacterial nosocomial infection with severe drug resistance. Hemolysin co-regulated protein (Hcp) is a marker of activated type VI secretion system (T6SS), a key secretory system that promotes Gram-negative bacteria colonization, adhesion, and invasion of host cells. Hcp is also regulated by iron ions (Fe).

Quantifying the Impact of Urban Form and Socio-Economic Development on China's Carbon Emissions.

Carbon emissions (CEs) are one of the most important factors causing global warming. The development of social economy and the acceleration of the urbanization process leads to increasing CEs, especially in China. Therefore, it has become an international community consensus to control the growth of CEs and mitigate global warming.

Image Encryption Scheme Based on Mixed Chaotic Bernoulli Measurement Matrix Block Compressive Sensing.

Many image encryption schemes based on compressive sensing have poor reconstructed image quality when the compression ratio is low, as well as difficulty in hardware implementation. To address these problems, we propose an image encryption algorithm based on the mixed chaotic Bernoulli measurement matrix block compressive sensing. A new chaotic measurement matrix was designed using the Chebyshev map and logistic map; the image was compressed in blocks to obtain the measurement values.

Double-Matrix Decomposition Image Steganography Scheme Based on Wavelet Transform with Multi-Region Coverage.

On the basis of ensuring the quality and concealment of steganographic images, this paper proposes a double-matrix decomposition image steganography scheme with multi-region coverage, to solve the problem of poor extraction ability of steganographic images under attack or interference. First of all, the cover image is transformed by multi-wavelet transform, and the hidden region covering multiple wavelet sub-bands is selected in the wavelet domain of the cover image to embed the secret information. After determining the hidden region, the hidden region is processed by Arnold transform, Hessenberg decomposition, and singular-value decomposition.

Palladium-Catalyzed Migratory Insertion of Carbenes and C-C Cleavage of Cycloalkanecarboxamides.

A palladium catalyzed reaction of cycloalkanecarboxamides and diazomalonates or bis(phenylsulfonyl)diazomethane has been developed. The reaction proceeds via carbene migratory insertion and cascade C-C cleavage pathways. Cycloalkanecarboxamides with four to seven membered rings are applicable in the transformation.

Comparison of Cumulative Live Birth Rate Between Aged PCOS Women and Controls in IVF/ICSI Cycles.

The present study aimed to assess whether women with polycystic ovarian syndrome (PCOS) ≥35 years age undergoing fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles experienced a higher cumulative live birth rate (CLBR) over a two-year period compared with age- and body mass index (BMI)-matched patients with tubal factor infertility. Through propensity score matching (PSM) approach, the authors retrospectively analyzed the IVF/ICSI outcomes of 263 PCOS patients (35-46 years of age [mean, 37 years]) and 526 age- and BMI-matched tubal factor controls two years after oocyte retrieval. Multivariate regression analysis was performed to explore factors influencing cumulative live birth.

Plaintext-Related Dynamic Key Chaotic Image Encryption Algorithm.

To address the problems of the high complexity and low security of the existing image encryption algorithms, this paper proposes a dynamic key chaotic image encryption algorithm with low complexity and high security associated with plaintext. Firstly, the RGB components of the color image are read, and the RGB components are normalized to obtain the key that is closely related to the plaintext, and then the Arnold transform is used to stretch and fold the RGB components of the color image to change the position of the pixel points in space, so as to destroy the correlation between the adjacent pixel points of the image. Next, the generated sequences are independently encrypted with the Arnold-transformed RGB matrix.

Characterization of Tbr2-expressing retinal ganglion cells.

The mammalian retina contains more than 40 retinal ganglion cell (RGC) subtypes based on their unique morphologies, functions, and molecular profiles. Among them, intrinsically photosensitive RGCs (ipRGCs) are the first specified RGC type emerging from a common retinal progenitor pool during development. Previous work has shown that T-box transcription factor T-brain 2 (Tbr2) is essential for the formation and maintenance of ipRGCs, and that Tbr2-expressing RGCs activate Opn4 expression upon native ipRGC ablation, suggesting that Tbr2 RGCs contain a reservoir for ipRGCs.

Successful live birth in a Chinese woman with P450 oxidoreductase deficiency through frozen-thawed embryo transfer: a case report with review of the literature.

Background: Congenital adrenal hyperplasia (CAH) caused by P450 oxidoreductase deficiency (PORD) in 46, XX patients is characterized by genital ambiguity, primary amenorrhea, absent or incomplete sexual maturation, infertility, skeletal malformations and so on. But few pregnancies have been reported from these female patients with PORD.

Case Description: A 29-year-old Chinese woman with PORD due to the compound heterozygous mutation (c.