Water loss from silicone tubing and effect on protein concentration during drug product manufacturing.

Silicone tubing is used in various unit operations during drug product (DP) manufacturing. Hold of protein formulations in silicone tubing over time may have an impact on product quality, particularly protein concentration. This study evaluated the change in protein concentration of a test monoclonal antibody (mAb) formulation over various hold times in silicone tubing as a function of tubing internal diameter (ID) and wall thickness.

Examining Acid Formation During the Selective Dehydration of Fructose to 5-Hydroxymethylfurfural in Dimethyl Sulfoxide and Water.

Sustainable conversion of biomass, including fructose dehydration to 5-hydroxymethylfurfural (HMF), remains a challenge. Fructose can be selectively dehydrated to HMF in dimethyl sulfoxide (DMSO) without addition of an acid catalyst. The role of DMSO is examined starting with either fructose or HMF in DMSO/water.