The study addresses the detection of α-pyrrolidinovalerophenone (α-PVP) and its metabolites in urine, highlighting the challenge of their amphoteric properties which complicate analysis via gas chromatography-mass spectrometry (GC/MS).
An automated solid-phase extraction (SPE) method was optimized, showing significant improvement in derivatization efficiency when adding pyridine to the reaction mixture, which enhanced sensitivity and precision of detection.
The developed method yielded a linear range of detection and successfully identified α-PVP and its metabolite in a forensic sample, marking a novel approach for analyzing synthetic cathinones in biological specimens.