The strategic uses of collagen in adherent cell cultures.

The culture of adherent mammalian cells involves adhesion to the tissue culture vessel. This requires attachment factors from serum and/or a suitable substrate on the vessel surface. Some cells require collagen or other substrates to promote neurite outgrowth, differentiation or growth.

Phytochemicals from Hook f. ex T. Anderson and their neuroprotective effects.

Previous phytochemical investigations reported that have biosynthesized a wide range of bioactive phenolics such as xanthones and coumarins. The phytochemical study conducted on the stem bark of has led to the isolation of eight trioxygenated xanthones namely: 5-methoxytrapezifolixanthone , 5-methoxyananixanthone , caloxanthone C , 1,5-dihydroxy-3-methoxy-4-isoprenylxanthone , 6-deoxyisojacareubin , euxanthone , trapezifolixanthone , ananixanthone , together with three common triterpenoids, -sitosterol , friedelin , and stigmasterol . Furthermore, xanthones and were isolated for the first time as naturally occurring xanthones from the plant extract.

Mobile health applications: awareness, attitudes, and practices among medical students in Malaysia.

Background: The popularity of mobile health (mHealth) applications (or apps) in the field of health and medical education is rapidly increasing, especially since the COVID-19 pandemic. We aimed to assess awareness, attitudes, practices, and factors associated with the mHealth app usage among medical students.

Methods: We conducted a cross-sectional study involving medical students at a government university in Sarawak, Malaysia, from February to April 2021.

Optimisation of a PC12 cell-based in vitro stroke model for screening neuroprotective agents.

Stroke causes death and disability globally but no neuroprotectant is approved for post-stroke neuronal injury. Neuroprotective compounds can be identified using oxygen glucose deprivation (OGD) of neuronal cells as an in vitro stroke model. Nerve growth factor (NGF)-differentiated PC12 pheochromocytoma cells are frequently used.

Acute decompensated heart failure in a non cardiology tertiary referral centre, Sarawak General Hospital (SGH-HF).

Background: Data on clinical characteristics of acute decompensated heart failure (ADHF) in Malaysia especially in East Malaysia is lacking.

Methods: This is a prospective observational study in Sarawak General Hospital, Medical Department, from October 2017 to September 2018. Patients with primary admission diagnosis of ADHF were recruited and followed up for 90 days.