A Fully-Printed Wearable Bandage-Based Electrochemical Sensor with pH Correction for Wound Infection Monitoring.

Wearable sensing systems have been designed to monitor health conditions in real-time by detecting analytes in human biofluids. Wound diagnosis remains challenging, necessitating suitable materials for high-performance wearable sensors to offer prompt feedback. Existing devices have limitations in measuring pH and the concentration of pH-dependent electroactive species simultaneously, which is crucial for obtaining a comprehensive understanding of wound status and optimizing biosensors.

The potential of turmeric extract-loaded chitosan microparticles for the treatment of gastrointestinal disorders.

Aim: To develop turmeric extract-loaded chitosan microparticles for treating gastrointestinal disorders.

Methods: The microparticles were prepared using a spray-drying process, optimised the characteristics by biomarker loading, and encapsulation efficiency, and assessed for bioactivities related to gastrointestinal diseases.

Results: The optimised microparticles were spherical, with a mean diameter of 2.

Characterization and Genome Analysis of Vibrio campbellii Lytic Bacteriophage OPA17.

Vibrio campbellii is a marine bacterium that is associated with luminous vibriosis, especially in the hatchery and nursery stages of penaeid shrimp cultivation worldwide, which has led to low survival rates of shrimp during aquaculture. Phage therapy has been reported as an alternative biocontrol agent which can reduce or replace the use of antibiotics and other chemicals. This study characterized a lytic bacteriophage, OPA17, originally isolated from bloody clams and investigated its biocontrol efficacy against infection in a model system, Artemia franciscana.

Complete Genome Sequence of NH9449 and Comprehensive Comparative-Genomic Analysis: Genomic Diversity and Versatility Trait Revealed.

Lactic acid bacteria (LAB) in the genus spp. contain traits in their genome that confer versatility. In particular, encodes several beneficial genes that are useful in biotechnological applications.

Phytochemical Composition and Bioactivities of Aqueous Extract of Rambutan ( L. cv. Rong Rian) Peel.

Thailand is one of the leading exporting countries of rambutan and rambutan peels are considered as a biological waste. In this study, rambutan ( L. cv.

Characterization and Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in Pandemic and Non-Pandemic Isolates from Seafood Sources.

is one of the significant seafood-borne pathogens causing gastroenteritis in humans. Clustered regularly interspaced short palindromic repeats (CRISPR) are commonly detected in the genomes of and the polymorphism of CRISPR patterns has been applied as a genetic marker for tracking its evolution. In this work, a total of 15 pandemic and 36 non-pandemic isolates obtained from seafood between 2000 and 2012 were characterized based on hemolytic activity, antimicrobial susceptibility, and CRISPR elements.

influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing .

Background: Acute hepatopancreatic necrosis syndrome (AHPND) caused by strain (VP) impacts the shrimp industry worldwide. With the increasing problem of antibiotic abuse, studies on quorum sensing (QS) system and anti-QS compounds bring potential breakthroughs for disease prevention and treatment.

Methods: In this study, the cell-free culture supernatant (CFCS) and its extract of BC25 were investigated for anti-QS activity against a reporter bacteria, DMST46846.

Non-Invasive Colorimetric Magneto Loop-Mediated Isothermal Amplification (CM-LAMP) Method for Detection.

More than half the world's population is thought to be infected with . Although the majority of infected people are asymptomatic, infection may cause gastric ulcers and deadly gastric cancer. Owing to the difficulty and invasiveness of current routine culture and diagnostic methods, a highly sensitive and specific noninvasive assay for is of interest.

Induction and Genome Analysis of HY01, a Newly Reported Prophage from an Emerging Shrimp Pathogen .

is an emerging aquaculture pathogen that causes luminous vibriosis in farmed shrimp. Although prophages in various aquaculture pathogens have been widely reported, there is still limited knowledge regarding prophages in the genome of pathogenic . Here, we describe the full-genome sequence of a prophage named HY01, induced from the emerging shrimp pathogen HY01.

Indole signaling decreases biofilm formation and related virulence of Listeria monocytogenes.

Bacterial communication system known as quorum sensing (QS) is a pivotal system for bacterial survival, adaptation and pathogenesis. Members in the multicellular community may synthesize or acquire a signaling molecule in order to elicit downstream cellular processes. Roles of indole and derivatives, a new class of quorum-sensing signal molecules, in various bacterial physiologies and virulence have been reported recently.

Characterization and CRISPR-based genotyping of clinical -positive .

Background: is a causative agent of gastroenteritis. Most of the clinical isolates carry either and/or genes which are considered as the major virulence genes of this pathogen. In this study, the clinical isolates of carrying gene (= 73) obtained from 1886 to 2012 from various countries were investigated for the urease production, haemolytic activity, and biofilm formation.

Multiplex Multilocus Variable-Number Tandem-Repeat Analysis for Typing of Pandemic Vibrio parahaemolyticus O1:KUT Isolates.

Pandemic O3:K6 Vibrio parahaemolyticus emerged in 1996. Since then, this strain of pathogen and its serovariants (predominantly O1:KUT [untypable], O1:K25 and O4:K68) have caused gastroenteritis worldwide. Owing to the limitation in established K antisera, tracking the sources of KUT for epidemiological investigation is difficult.

Comparison of virulence gene profiles and genomic fingerprints of O1 and non-O1/non-O139 isolates from diarrheal patients in southern Thailand.

Background: is associated with severe watery diarrheal disease among people in many parts of the world, including the coastal provinces of Southern Thailand. There are relatively few studies focusing on the genetic characterization among isolates in this region. Therefore, this study aimed at exploring the presence of virulence genes and DNA fingerprints among O1 and nonO1/nonO139 isolates obtained from clinical samples in four southern coastal provinces during the period of 20012009 ( = 21).

Correction to: CRISPR-like sequences in and application in genotyping.

[This corrects the article DOI: 10.1186/s13099-017-0215-8.].

CRISPR-like sequences in and application in genotyping.

Background: Many bacteria and archaea possess a defense system called clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (CRISPR-Cas system) against invaders such as phages or plasmids. This system has not been demonstrated in . The numbers of spacer in CRISPR array differ among bacterial strains and can be used as a genetic marker for bacterial typing.

Isolation and characterization of Vibrio cholerae isolates from seafood in Hat Yai City, Songkhla, Thailand.

Seafood has been identified as an important source of Vibrio cholerae in Thailand, especially in the Southern coastal region. In this study, we isolated and characterized V. cholerae from seafood obtained from several markets in Hat Yai city, Southern Thailand.

The Trend of Vibrio parahaemolyticus Infections in Southern Thailand from 2006 to 2010.

The bacterium, Vibrio parahaemolyticus was isolated from 776 patients at Hat Yai Hospital in Southern Thailand from 2006 to 2010. 51.3-73.

Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness.

Melanization due to the inactivation of the homogentisate-1,2-dioxygenase gene (hmgA) has been demonstrated to increase stress resistance, persistence, and virulence in some bacterial species but such pigmented mutants have not been observed in pathogenic members of the Vibrio Harveyi clade. In this study, we used Vibrio campbellii ATCC BAA-1116 as model organism to understand how melanization affected cellular phenotype, metabolism, and virulence. An in-frame deletion of the hmgA gene resulted in the overproduction of a pigment in cell culture supernatants and cellular membranes that was identified as pyomelanin.

Occurrence of potentially pathogenic vibrios and related environmental factors in Songkhla Lake, Thailand.

Vibrios are halophilic bacteria that are ubiquitous in marine environments. Their occurrence in tropical lakes has rarely been investigated. In this study, the predominance and diversity of Vibrio spp.