Hyper-Expandable Cross-Linked Protein Crystals as Scaffolds for Catalytic Reactions.

Scaffolding catalytic reactions within porous materials is a powerful strategy to enhance the reaction rates of multicatalytic systems. However, it remains challenging to develop materials with high porosity, high diversity of functional groups within the pores, and guest-adaptive tunability. Furthermore, it is challenging to capture large catalysts such as enzymes within porous materials.

Impact of adding warm asphalt mix additives on recycling milled coatings: performance evaluation.

The use of warm mix asphalt (WMA) and reclaimed asphalt pavement (RAP) technologies presents challenges in optimizing binder activation and mechanical performance in asphalt mixtures. This study aimed to evaluate the effects of three WMA additives (sunflower oil, WarmGrip®, and natural zeolite) and different RAP contents (30% and 70%) on the rheological and mechanical properties of recycled asphalt mixtures. The research focused on assessing the degree of RAP binder activation, determining the extent of partial activation, and analyzing the impact on tensile strength, moisture resistance, modulus, fatigue life, and deformation resistance.

Metabolomic Insights into COVID-19 Severity: A Scoping Review.

Background: In 2019, SARS-CoV-2, the novel coronavirus, entered the world scene, presenting a global health crisis with a broad spectrum of clinical manifestations. Recognizing the significance of metabolomics as the omics closest to symptomatology, it has become a useful tool for predicting clinical outcomes. Several metabolomic studies have indicated variations in the metabolome corresponding to different disease severities, highlighting the potential of metabolomics to unravel crucial insights into the pathophysiology of SARS-CoV-2 infection.

Optimizing Corn Crop Protection: The First Sampling Plan for Controlling (Hemiptera: Cicadellidae).

Corn () is the most widely planted crop in the world. (Hemiptera: Cicadellidae) is currently a primary corn pest. The starting point for the development of pest control decision-making systems is the determination of a conventional sampling plan.

Definition of metafounders based on population structure analysis.

Background: Limitations of the concept of identity by descent in the presence of stratification within a breeding population may lead to an incomplete formulation of the conventional numerator relationship matrix ( ). Combining with the genomic relationship matrix ( ) in a single-step approach for genetic evaluation may cause inconsistencies that can be a source of bias in the resulting predictions. The objective of this study was to identify stratification using genomic data and to transfer this information to matrix , to improve the compatibility of and .

Group Movement in Entomopathogenic Nematodes: Aggregation Levels Vary Based on Context.

Maintenance of an aggregated population structure implies within-species communication. In mixed-species environments, species-specific aggregations may reduce interspecific competition and promote coexistence. We studied whether movement and aggregation behavior of three entomopathogenic nematode species changed when isolated, as compared to mixed-species arenas.

Toxicity evaluation of novel imidacloprid nanoribbons, using somatic mutation and fitness indexes in .

Nanoribbons of imidacloprid, a systemic and chloronicotinyl insecticide, were successfully synthesized by laser-induced fragmentation/exfoliation of imidacloprid powders suspended in water, with widths ranging from 160 to 470 nm, lengths in the micron scale, and thickness of a few atoms layers. The aim of the present study was to examine the effects of acute and chronic exposure to imidacloprid (IMC) bulk and compare its effects with synthesized imidacloprid nanoribbons (IMCNR) on larval and adult viability, developmental time, olfactory capacity, longevity, productivity, and genotoxicity in . Larvae or adults were exposed at 0.

Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed.

Natural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium, MTT, in cell culture of fibroblasts L929, human keratinocytes HaCat, and human endothelium EA.

Identification of Mayaro Virus Genotype D in Rondônia, Brazil.

The Mayaro virus (MAYV) is a significant reemerging arbovirus of public health concern, responsible for outbreaks in several countries including Brazil. In this study, 857 samples of patients with acute fever in the state of Rondônia, Brazil, were analyzed by reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) to detect Zika, dengue, and chikungunya viruses. The mean age of the population was 38 years (SD = 17.

The 235-360 GHz Rotational Spectrum of 1-Oxaspiro[2.5]octa-4,7-dien-6-one─Analysis of the Ground Vibrational State and Its 10 Lowest-Energy Vibrationally Excited States.

The millimeter-wave rotational spectrum of 1-oxaspiro[2.5]octa-4,7-dien-6-one () was collected from 235 to 360 GHz. With the rotational spectrum of this compound available for the first time, more than 5500 - and -type transitions were observed and assigned for the vibrational ground state.

How pedigree errors affect genetic evaluations and validation statistics.

Pedigrees used in genetic evaluations contain errors. Because of such errors, assumptions regarding the relatedness among individuals in genetic evaluation models are wrong. Consequences of that have been investigated in earlier studies focusing on models that did not account for genomic information yet.

DNA-Compatible Copper/TEMPO Oxidation for DNA-Encoded Libraries.

Aldehydes are important synthons for DNA-encoded library (DEL) construction, but the development of a DNA-compatible method for the oxidation of alcohols to aldehydes remains a significant challenge in the field of DEL chemistry. We report that a copper/TEMPO catalyst system enables the solution-phase DNA-compatible oxidation of DNA-linked primary activated alcohols to aldehydes. The semiaqueous, room-temperature reaction conditions afford oxidation of benzylic, heterobenzylic, and allylic alcohols in high yield, with DNA compatibility verified by mass spectrometry, qPCR, Sanger sequencing, and ligation assays.

Genotoxic action of bifenthrin nanoparticles and its effect on the development, productivity, and behavior of .

Rapid development of nanotechnology, particularly nanoparticles of pesticides, has facilitated the transformation of traditional agriculture. However, testing their effectiveness is essential for avoiding any environmental or adverse human health risk attributed to nanoparticle-based formulations, especially insecticides. Recently, organic nanoparticles of bifenthrin, a pyrethroid insecticide, were successfully synthesized by laser ablation of solids in liquid technique, with the most probable size of 5 nm.

Effects of in vitro erosion on surface texture, microhardness, and color stability of resin composite with S-PRG fillers.

Objectives: To evaluate the effect of acid erosion on different physical properties of resin composite with S-PRG (surface pre-reacted glass) fillers, by conducting simulations of intrinsic and extrinsic sources.

Materials And Methods: Cylindrical samples (Ø6 vs. 2 mm) of a conventional nanohybrid resin composite (Forma, Ultradent) and of a nanohybrid resin composite with S-PRG filler (Beautifil II, Shofu) were exposed to erosive cycling (5 days), based on (n=12) remineralizing solution (control); 0.

Fibropapillomatosis Prevalence and Distribution in Immature Green Turtles (Chelonia mydas) in Martinique Island (Lesser Antilles).

Fibropapillomatosis (FP) threatens the survival of green turtle (Chelonia mydas) populations at a global scale, and human activities are regularly pointed as causes of high FP prevalence. However, the association of ecological factors with the disease's severity in complex coastal systems has not been well established and requires further studies. Based on a set of 405 individuals caught over ten years, this preliminary study provides the first insight of FP in Martinique Island, which is a critical development area for immature green turtles.

Relationship between lifespan and somatic mutation in D. melanogaster after treatment with chlorophyllin.

Sodium copper chlorophyllin (SCC) has a genetic damage inhibitory capacity due to its antioxidant action. For this reason, it was considered to investigate its role in the life span of Drosophila melanogaster and its relationship with the frequency of somatic mutation induced by gamma rays. Results indicated that SCC alone prolonged the lifespan only in females, but in combination with 20 Gy of gamma rays, the aging delay in both sexes was significant.

Stability of a Natural-Based Cream Containing Extract.

The outer pericarps of Camb. are by-products with a high content of antioxidant substances. The goal of the present study was to analyze the incorporation of pericarp extract in an all-natural formulation.

DNA-Scaffolded Synergistic Catalysis.

We report DNA-scaffolded synergistic catalysis, a concept that combines the diverse reaction scope of synergistic catalysis with the ability of DNA to precisely preorganize abiotic groups and undergo stimuli-triggered conformational changes. As an initial demonstration of this concept, we focus on Cu-TEMPO-catalyzed aerobic alcohol oxidation, using DNA as a scaffold to hold a copper cocatalyst and an organic radical cocatalyst (TEMPO) in proximity. The DNA-scaffolded catalyst maintained a high turnover number upon dilution and exhibited 190-fold improvement in catalyst turnover number relative to the unscaffolded cocatalysts.

In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI-MS spectrum.

Subunit vaccines based on the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 provide one of the most promising strategies to fight the COVID-19 pandemic. The detailed characterization of the protein primary structure by mass spectrometry (MS) is mandatory, as described in ICHQ6B guidelines. In this work, several recombinant RBD proteins produced in five expression systems were characterized using a non-conventional protocol known as in-solution buffer-free digestion (BFD).