Rapid detection of methicillin-resistant Staphylococcus aureus in positive blood-cultures by recombinase polymerase amplification combined with lateral flow strip.

Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), is an important bacterium that causes community and healthcare-related infections throughout the world. However, the current conventional detection methods are time-consuming.

Rapid detection of and vancomycin resistance using recombinase polymerase amplification.

Vancomycin-resistant enterococci (VRE), especially , have been a global concern, often causing serious healthcare-associated infections. We established a rapid approach for detecting and vancomycin-resistance genes ( and ) in clinical samples using isothermal recombinase polymerase amplification (RPA) combined with a lateral-flow (LF) strip. Specific RPA primer sets and probes for (to identify the presence of ) and genes were designed.

Effect of Vancomycin on Cellular Fatty Acid Profiles of Vancomycin-Susceptible and Nonsusceptible .

Vancomycin is widely used for treatment of infection caused by methicillin-resistant (MRSA) leading to an increasing appearance of low-level vancomycin-resistant isolates called heterogeneous vancomycin-intermediate (hVISA). The mechanism of vancomycin tolerance in hVISA is still unclear. This study aimed to investigate the fatty acid compositions of isolates under the stress environment with vancomycin.