Analysis of conjugated estrogens in a vaginal cream formulation by capillary gas chromatography.

A capillary gas-chromatographic method is described for the quantitative analysis of nine equine estrogens in a vaginal cream formulation. The sodium sulfate ethers of the estrogens were selectively extracted from the formulation, subjected to enzyme hydrolysis, and derivatized to their oxime-trimethylsilyl esters. Resolution of the resulting derivatives was achieved on short (15 m) capillary column, wall-coated with cyanopropylmethyl silicon stationary phase.

Electron-capture gas-liquid chromatographic determination of tocainide in biological fluids using fused silica capillary columns.

Gas-liquid chromatography using capillary columns and electron-capture detection has been employed for the determination of a new antiarrhythmic drug, tocainide, in rat plasma and urine. The drug is extracted from a basified solution along with an internal standard, monoethylglycine xylidide, and subsequently reacted with heptafluorobutyric anhydride. The 50 m x 0.

Quantitative determination of conjugated estrogens in formulations by capillary GLC.

A rapid capillary GLC method for the analysis of conjugated estrogen tablets and injectable formulations is described. The method involves the hydrolytic cleavage of the sodium sulfate ester conjugates by sulfatase enzyme. The free phenolic steroids are reacted sequentially with hydroxylamine hydrochloride and N,O-bis(trimethylsilyl)trifluoroacetamide.