Chemically modified alginate bead matrix for efficient adsorptive recovery of trypsin from fresh bovine pancreas.

The adsorption of commercial trypsin (Try) onto epichlorohydrin cross-linked alginate-guar gum matrix has been studied at equilibrium in batch and in fixed bed column. Experiments were conducted to study the effect of ionic strength, temperature and to obtain a thermodynamic characterization of the adsorption process. The resulting adsorption isotherm fitted the Hill equation.