Publications by authors named "Pietro P Fanciulli"

Article Synopsis
  • The sperm cells of the diving beetle Deronectes moestus incospectus form bundles of 64 units through a process called sperm conjugation, which happens during cell division in the testes.
  • These sperm bundles, found in the deferent ducts, are surrounded by an extracellular cup that helps protect and organize the sperm, but this material disappears further down the duct.
  • The structure of the sperm includes a unique axoneme arrangement and accessory bodies, with the extracellular material showing varied forms across different families of beetles, suggesting a common function regardless of specific species.
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The fine structure of the female reproductive organs of the diving beetle has been described, with particular attention to the complex organization of the spermatheca and the spermathecal gland. These organs are fused in a single structure whose epithelium is involved in a quite different activity. The secretory cells of the spermathecal gland have a large extracellular cistern with secretions; duct-forming cells, by their efferent duct, transport the secretions up to the apical cell region where they are discharged into the gland lumen.

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The general organization of the female genital system of the diving beetle Stictonectes optatus was studied, clarifying the complex structure of the spermatheca and spermathecal gland. The two structures adhere closely to each other, sharing a small area of their cuticular epithelium. A long duct connects the bursa copulatrix to the spermatheca, where the sperm are stored.

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The structure of the male genital organs and spermiogenesis of two diving beetles, Stictonectes optatus and Scarodytes halensis were studied for the first time. S. optatus shows unifollicular testes consisting of a long tubule apically forming a globular structure.

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The sperm ultrastructure of a few representative species of Tenebrionoidea was studied. Two species belong to the Mordellidae ( sp.), one species to Oedemeridae (), and one species to Tenebrionidae ().

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The sperm ultrastructure of some beetles of Tenebrionoidea was studied with particular attention to those of the Ripiphoridae, Mordellidae, and Meloidae. These three groups are often thought to form a clade, which is the sister group of the remaining Tenebrionoidea. The testes of the two former families have thinner but longer spermatic cysts containing fewer and longer sperm.

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Article Synopsis
  • The mitochondrial genome of a true bug from the Homoptera order is analyzed and compared with other species from the Lygaeoidea superfamily.
  • Its mtDNA shows typical gene order and molecular characteristics found in hexapods, including a long A+T-rich region with many repeat units.
  • Phylogenetic analysis indicates that all families are monophyletic, except for the Rhyparochromidae family.
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Article Synopsis
  • - The study analyzes the sperm structure of *Pytho depressus*, detailing its size, acrosome layers, cylindrical nucleus, and unique flagellum features.
  • - The sperm morphology of *P. depressus* is distinct from that of related families like Ripiphoridae, Meloidae, and Tenebrionidae, helping identify species within the superfamily.
  • - The research also discusses the evolutionary significance of the sperm characteristics, comparing them to the sperm structure of various other insect species.
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Complete mitochondrial genome data are frequently applied to address phylogenetic/phylogeographic issues at different taxonomic levels in ecology and evolution. While sample preparation/sequencing is becoming more and more straightforward thanks to dropping costs for next-generation sequencing (NGS), data preparation and visualization remains a manually intensive step that may lead to errors if improperly conducted. We have elaborated, and here introduce, EZmito, a simple and intuitive, freely accessible Web Server aimed at automating some of these tasks.

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Article Synopsis
  • The reproductive systems of male and female water-striders (Gerris lacustris) were analyzed, focusing on their structural features and functions.
  • Male sperm is noted for its unique helicoidal acrosome and specific flagellum structure, while the female has a long spermathecal duct that serves as the primary organ for sperm storage.
  • The study indicates a potential evolutionary link between the lengths of sperm and the spermathecal duct, suggesting they may have adapted in response to each other over time.
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Article Synopsis
  • Symphypleona are a type of springtails (Collembola) that have not been thoroughly studied in terms of their evolutionary relationships and gene order variations.
  • The research involved sequencing the complete mitochondrial genome of Sminthuridae, a family within Symphypleona, to provide new insights.
  • This genomic data is utilized to better understand the phylogenetic connections among different families and subfamilies within Symphypleona.
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Collembola are an ancient and early diverging lineage of basal hexapods that occur in virtually all terrestrial habitats on Earth. Phylogenetic relationships between the different orders of Collembola are fiercely debated. Despite a range of studies and the application of both morphological and genetic approaches (singly or in combination) to assess the evolutionary relationships of major lineages in the group, no consensus has been reached.

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Article Synopsis
  • - The study examines the coevolution of sperm length and female sperm-storage organ size in the measurer bug, *Hydrometra stagnorum*, marking a first for the Heteroptera group.
  • - The bug's sperm has a notably long acrosome and a very short nucleus, along with a flagellum featuring a typical axoneme structure and unique mitochondrial characteristics.
  • - The female's reproductive system includes a long spermathecal duct and a bulb, with secretions from a short gland, indicating that the size of these organs influences the shape and length of the sperm.
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  • - The retreat of glaciers creates "natural laboratories" for studying how arthropods establish themselves and form food webs in newly exposed environments before vascular plants appear.
  • - Research comparing glacial forelands in northern Europe and the Alps reveals that springtails are key early colonizers, feeding on biofilms rather than the previously assumed pioneer predators like ground beetles and spiders.
  • - The study indicates that pioneer arthropod communities and food web structures vary by region and climate, with some commonalities, but cold-adapted specialists are at risk due to climate change and glacier melting.
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  • Research on Antarctica's invertebrates is revealing how they adapt to extreme conditions and evolve over millions of years.
  • Advances in taxonomic techniques and molecular biology are leading to changes in species classification, including the springtail, previously thought to be the only pan-Antarctic species.
  • New studies indicate that populations once considered a single species are actually distinct and evolutionary isolated, suggesting the presence of at least two new species in Continental Antarctica.
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The sperm structure of several species belonging to different tribes of the large Carabidae family is described. Some species of Nebriinae, such as Nebria brevicollis and Notiophilus biguttatus, have free conventional insect sperm. Their sperm type can be regarded as the ancestral model for Carabidae.

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Article Synopsis
  • The complete mitochondrial genome of the springtail (Fitch, 1863) is described and used for a Bayesian phylogenetic analysis, which includes all known sequenced mitochondrial DNAs from the Collembola group.
  • The study confirms that the gene content, order, and nucleotide composition of this springtail's mitochondrial DNA align with typical features found in hexapods.
  • The phylogenetic analysis validates the monophyly of various Collembola subgroups, but the absence of mitochondrial DNA from Neelipleona limits further research into springtail classification.
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Article Synopsis
  • The complete mitochondrial genome of the machilid species is described and utilized for phylogenetic analyses of early-diverging hexapod lineages.
  • The gene organization is generally consistent with other arthropods, but features a unique translocation and an unusually long A + T-rich region due to repeat units.
  • The phylogenetic findings affirm the expected relationships among hexapods, positioning Ectognatha, Monocondylia, and Dicondylia together, with Diplura being the closest relative to true insects.
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Article Synopsis
  • - The study focuses on Antarctic collembolan species, specifically re-evaluating their physical and molecular traits due to increasing interest in them.
  • - The researchers designate a neotype for Folsomotoma octooculata and provide a detailed description of its morphological features, particularly its sensory structures.
  • - They also offer a systematic key for identifying different species within the Folsomotoma genus based on new and previously collected morphological data.
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Article Synopsis
  • A new species of cave-dwelling organism has been identified in northern Italy.
  • Researchers used specific characteristics, like size and the number of certain body features, to differentiate it from similar species.
  • Key identifying traits include the distribution of dorsal and ventral pseudocelli and the number of compound vesicles in the post-antennal organ.
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The sperm structure of the jumping bristletail Machilontus sp has been described. The species shares several sperm characteristics with other genera of the same order Archaeognatha. During late spermiogenesis the spermatid bends at half of its length with the two limbs closely apposed within the same plasma membrane.

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Despite its ancient origin, global distribution and abundance in nearly all habitats, the class Collembola is comprised of only 8000 described species and is estimated to number no more than 50,000. Many morphologically defined species have broad geographical ranges that span continents, and recent molecular work has revealed high genetic diversity within species. However, the evolutionary significance of this genetic diversity is unknown.

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Podura aquatica is a springtail of uncertain systematic position. Our study dealing with the ultrastructure of the spermatogenesis and oogenesis of this species is a contribution to a better knowledge of both the reproduction and the systematics of the taxon. In the male, the spermatogenesis proceeds in a similar way to that of other Collembola.

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The initial part of the ejaculatory duct of Orchesella villosa contains a "valve" and a "sorter" avoiding respectively the reflow and allowing the separation of the secretion for the spermatophore stalk from the sperm fluid. For most of its length, the ejaculatory duct lumen is divided into two parts: in the dorsal part the sperm fluid flows while in the ventral district the secretion for the stalk occurs. Laterally, on both sides of the duct, longitudinal muscle fibers are present.

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