Quantification of hydrolysis activity in a biological wastewater treatment context.

This paper reviews currently available methods for hydrolysis activity monitoring of the most commonly encountered enzyme categories in biological wastewater treatment. While highlighting the relevant methods for protein, lipid, carbohydrate, organic phosphate, and ester hydrolysis, the discussion of their pros and cons is predominantly aimed at revealing the relevance of the to-be-hydrolyzed substrates that are used in the methods. These "substrates" should mimic the proteins, lipids, or other polymers that are present in the wastewater and are in the reviewed methods (i) real substrates (i.

A high-throughput assay to quantify protein hydrolysis in aerobic and anaerobic wastewater treatment processes.

Proteins, an important fraction of the organic matter in wastewater, typically enter a treatment facility as high molecular weight components. These components are degraded by extracellular protein hydrolytic enzymes, denoted as proteases. Adequate protein hydrolysis monitoring is crucial, since protein hydrolysis is often a rate-limiting step in wastewater treatment.