Publications by authors named "Pierre Godessart"
The pathogenic bacterium Brucella abortus invades and multiplies inside host cells. To grow inside host cells, B. abortus requires a functional histidine biosynthesis pathway.
View Article and Find Full Text PDFGram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial for envelope integrity in Escherichia coli. When the OM is not attached to the PG, the OM forms blebs and detaches from the cell.
View Article and Find Full Text PDFArticle Synopsis
- - The study focuses on a class III zoonotic bacterial pathogen that can survive and replicate within host cells, particularly macrophages, by using a detailed transposon sequencing analysis to identify essential genes.
- - Researchers created a comprehensive transposon mutant library and mapped nearly a million insertion sites, revealing 491 essential coding sequences, with chromosome I containing a higher percentage of essential genes than chromosome II.
- - They discovered 48 crucial genes for the bacteria's growth inside macrophages, highlighting the importance of the pyrimidine nucleotide biosynthesis pathway for its proliferation in these immune cells.