Simultaneous determination of benzydamine hydrochloride and five impurities in an oral collutory as a pharmaceutical formulation by high-performance liquid chromatography.

A reversed-phase high-performance liquid chromatographic method for the determination of benzydamine hydrochloride and its impurities 3-dimethylaminopropyl 2-benzylaminobenzoate, 3-dimethylaminopropyl-2-aminobenzoate,1-benzyl-1H-indazol-3-ol, 1-benzyl-2-(3-dimethylaminopropyl)-1,2-dihydro-3H-indazol-3-one, and 1-benzyl-3-(3-(3-dimethylaminopropyl)-3-methylamino)propoxy-(1)H-indazole in a collutory formulation is developed. The separation is carried out on a Gemini C(18) (250 × 4.6 mm, 5 μm) column using acetonitrile-methanol-ammonium carbonate buffer (10 mM; pH 10.

RP-HPLC method with fluorescence detection for amino acids D/L ratio determination in fossil bones as a marker of DNA preservation.

Only a small proportion of old specimens contain DNA that can be amplified by PCR. Therefore, rapid screening methods are crucial to identify the large fraction of samples that are so badly preserved that there is no need to attempt DNA extraction. In particular, the extent of racemization of some amino acids has proved to be a very useful proxy for DNA preservation.

HPLC-DAD method for the simultaneous determination of zofenopril and hydrochlorothiazide in oral pharmaceutical formulations.

An HPLC method with DAD detection was developed and validated for the simultaneous determination of zofenopril and hydrochlorothiazide in tablets. The separation was carried out through a gradient elution using an Agilent LiChrospher C18 column (250x4.0 mm id, 5 microm) and a mobile phase consisting of (A) water-TFA (99.

Investigation of the HPLC response of NSAIDs by fractional experimental design and multivariate regression analysis. Response optimization and new retention parameters.

The optimization of the HPLC procedures and the investigation of the predictive models are generally seen as distinct tasks although they could be both approached by very similar chemometric methods. In this work we used chemometric approaches to both optimize the separation of a non-steroidal anti-inflammatory drugs (NSAIDs) set by isocratic reversed-phase HPLC and start-up the development of retention predictive models. The screening of responsive variables and the search for an optimal experimental domain provided also an insight into the structure-chromatographic response relationships for the considered compounds which were particularly helpful to derive new retention parameters.

Development of a method for the determination of vardenafil in human plasma by high performance liquid chromatography with UV detection.

A simple high-performance liquid chromatographic (HPLC) method with photometric detection is described for the determination of vardenafil hydrochloride, a phosphodiesterase V inhibitor, in human plasma. Chromatographic separation of the analyte and internal standard was achieved on an analytical 250 x 4.6 mm i.