In situ detection of ferric reductase activity in the intestinal lumen of an insect.

The rise of atmospheric oxygen as a result of photosynthesis in cyanobacteria and chloroplasts has transformed most environmental iron into the ferric state. In contrast, cells within organisms maintain a reducing internal milieu and utilize predominantly ferrous iron. Ferric reductases are enzymes that transfer electrons to ferric ions, either extracellularly or within endocytic vesicles, enabling cellular ferrous iron uptake through Divalent Metal Transporter 1.

Insight into the Cationic Charge Distribution in UPuAmO Mixed Oxides.

Uranium-plutonium mixed oxides, containing few mol % of Am, are currently studied as fuel for Sodium Fast Reactors. The study of the O/M ratio of these fuels is of main interest, as its variation can induce issues for reactor safety. For this purpose, four UPuAmO samples (0.

Molecular and Supramolecular Study of Uranium/Plutonium Liquid-Liquid Extraction with ,-Dialkylamides.

In the context of the separation of uranium and plutonium from spent fuel allowed by -dialkylamides, three regioisomers of -di(2-ethylhexyl) butyramide (DEHBA or ββ) and the diastereopure isomers of -(2-ethylhexyl)--(oct-3-yl)butyramide (EHOBA or αβ) were synthesized to assess their extraction performance and to study the mechanisms at the origin of the differences observed between the stereo- and regioisomers. The -dialkylamides showed differences in extraction, with a greater effect of regio- than stereoisomerism. A mechanistic study at both the molecular and supramolecular scales was initially applied to explain these effects.

Uranium contamination of bivalve Mytilus galloprovincialis, speciation and localization.

Uranium is a natural radioelement (also a model for heavier actinides), but may be released through anthropogenic activities. In order to assess its environmental impact in a given ecosystem, such as the marine system, it is essential to understand its distribution and speciation, and also to quantify its bioaccumulation. Our objective was to improve our understanding of the transfer and accumulation of uranium in marine biota with mussels taken here as sentinel species because of their sedentary nature and ability to filter seawater.

Exploring uranium bioaccumulation in the brown alga Ascophyllum nodosum: insights from multi-scale spectroscopy and imaging.

Legacy radioactive waste can be defined as the radioactive waste produced during the infancy of the civil nuclear industry's development in the mid-20th Century, a time when, unfortunately, waste storage and treatment were not well planned. The marine environment is one of the environmental compartments worth studying in this regard because of legacy waste in specific locations of the seabed. Comprising nearly 70% of the earth's service, the oceans are the largest and indeed the final destination for contaminated fresh waters.

Interaction Between the Transferrin Protein and Plutonium (and Thorium), What's New?

Transferrin (Tf) is a glycoprotein that transports iron from the serum to the various organs. Several studies have highlighted that Tf can interact with metals other than Fe(III), including actinides that are chemical and radiological toxics. We propose here to report on the behavior of Th(IV) and Pu(IV) in comparison with Fe(III) upon Tf complexation.

Uranium Speciation in a Chalky Soil.

In this article, the speciation and behavior of anthropogenic metallic uranium deposited on natural soil are approached by combining EXAFS (extended X-ray absorption fine structure) and TRLFS (time-resolved laser-induced fluorescence spectroscopy). First, uranium (uranyl) speciation was determined along the vertical profile of the soil and bedrock by linear combination fitting of the EXAFS spectra. It shows that uranium migration is strongly limited by the sorption reaction onto soil and rock constituents, mainly mineral carbonates and organic matter.

Optimized Coordination of Uranyl in Engineered Calmodulin Site 1 Provides a Subnanomolar Affinity for Uranyl and a Strong Uranyl versus Calcium Selectivity.

As an alpha emitter and chemical toxicant, uranium toxicity in living organisms is driven by its molecular interactions. It is therefore essential to identify main determinants of uranium affinity for proteins. Others and we showed that introducing a phosphoryl group in the coordination sphere of uranyl confers a strong affinity of proteins for uranyl.

Correction: Formation of plutonium(IV) silicate species in very alkaline reactive media.

Correction for 'Formation of plutonium(IV) silicate species in very alkaline reactive media' by Paul Estevenon , , 2021, , 12528-12536, DOI: 10.1039/D1DT02248B.

Characterization of a Hexanuclear Plutonium(IV) Nanostructure in an Acetate Solution via Visible-Near Infrared Absorption Spectroscopy, Extended X-ray Absorption Fine Structure Spectroscopy, and Density Functional Theory.

A new hexanuclear plutonium cluster has been stabilized in aqueous media with acetate ligands. To probe the formation of such a complex structure, visible-near infrared (vis-NIR) absorption spectroscopy, extended X-ray absorption fine structure (EXAFS) spectroscopy, and density functional theory (DFT) were combined. The presence of PuO(OH)(CHCOO) species in solution was first detected by vis-NIR and EXAFS spectroscopy.

Accumulation and Speciation of Cobalt in .

Since the first human release of radionuclides on Earth at the end of the Second World War, impact assessments have been implemented. Radionuclides are now ubiquitous, and the impact of local accidental release on human activities, although of low probability, is of tremendous social and economic consequences. Although radionuclide inventories (at various scales) are essential as input data for impact assessment, crucial information on physicochemical speciation is lacking.

Interaction of Th(IV), Pu(IV) and Fe(III) with ferritin protein: how similar?

Ferritin is the main protein of Fe storage in eukaryote and prokaryote cells. It is a large multifunctional, multi-subunit protein consisting of heavy H and light L subunits. In the field of nuclear toxicology, it has been suggested that some actinide elements, such as thorium and plutonium at oxidation state +IV, have a comparable `biochemistry' to iron at oxidation state +III owing to their very high tendency for hydrolysis and somewhat comparable ionic radii.

X-ray absorption spectroscopy and actinide electrochemistry: a setup dedicated to radioactive samples applied to neptunium chemistry.

A spectroelectrochemical setup has been developed to investigate radioactive elements in small volumes (0.7 to 2 ml) under oxidation-reduction (redox) controlled conditions by X-ray absorption spectroscopy (XAS). The cell design is presented together with in situ XAS measurements performed during neptunium redox reactions.

Formation of plutonium(IV) silicate species in very alkaline reactive media.

Studying the speciation of Pu(IV) in very alkaline and silicate ion rich reactive media allowed identification of the formation of plutonium(IV)-silicate colloidal suspensions which were stable for months. These colloids were stabilized in aqueous solution for pH > 13 and for concentrations around 10 mol L. Successive filtration processes allowed evaluation of their size, which was found to be smaller than 6 nm.

Structural and Thermodynamics Studies on Polyaminophosphonate Ligands for Uranyl Decorporation.

The development of actinide decorporation agents with high complexation affinity, high tissue specificity, and low biological toxicity is of vital importance for the sustained and healthy development of nuclear energy. After accidental actinide intake, sequestration by chelation therapy to reduce acute damage is considered as the most effective method. In this work, a series of bis- and tetra-phosphonated pyridine ligands have been designed, synthesized, and characterized for uranyl (UO) decorporation.

Uranium retention on iron oxyhydroxides in post-mining environmental conditions.

Investigating uranium migration mechanisms related to the weathering of waste rocks is essential for developing strategies that can address the potential environmental issues caused by uranium mining. This work is based on environmental samples containing 2 L ferrihydrite, lepidocrocite and goethite collected in the technosols from granitic waste rock piles, mine drainage conduits and mine waters. The results show the important role of iron oxyhydroxide in U immobilization and re-concentration.

How Does Iron Storage Protein Ferritin Interact with Plutonium (and Thorium)?

The impact of the contamination of living organisms by actinide elements has been a constant subject of attention since the 1950s. But to date still little is understood. Ferritin is the major storage and regulation protein of iron in many organisms, it consists of a protein ring and a ferrihydric core at the center.

High-efficient microbial immobilization of solved U(VI) by the Stenotrophomonas strain Br8.

The environmental impact of uranium released during nuclear power production and related mining activity is an issue of great concern. Innovative environmental-friendly water remediation strategies, like those based on U biomineralization through phosphatase activity, are desirable. Here, we report the great U biomineralization potential of Stenotrophomonas sp.

Electrochemical and Spectroscopic Study of Eu and Eu Coordination in the 1-Ethyl-3-methylimidazolium Bis(trifluoromethylsulfonyl)imide Ionic Liquid.

Separation processes based on room temperature ionic liquids (RTIL) and electrochemical refining are promising strategies for the recovery of lanthanides from primary ores and electronic waste. However, they require the speciation of dissolved elements to be known with accuracy. In the present study, Eu coordination and Eu /Eu electrochemical behavior as a function of water content in 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIm][NTf ]) was investigated using UV-visible spectrophotometry, time-resolved laser fluorescence spectroscopy, electrochemistry, and X-ray absorption spectroscopy.

Direct conversion of uranium dioxide UO to uranium tetrafluoride UF using the fluorinated ionic liquid [Bmim][PF].

The industrial fluorination of UO2 to UF4 is based on a complex process involving the manipulation of a large amount of HF, a very toxic and corrosive gas. We present here a safer way to accomplish this reaction utilizing ionic liquid [Bmim][PF6] as a unique reaction medium and fluoride source.

Carboxylate- and Phosphonate-Modified Polyethylenimine: Toward the Design of Actinide Decorporation Agents.

Plutonium (Pu) is an anthropogenic element involved in the nuclear industry cycle. Located at the bottom of the periodic table within the actinide family, it is a chemical toxic but also a radiological toxic, regardless of isotopy. After nearly 80 years of Pu industrialization, it has become clear that inhalation and wounds represent the two main ways a person may become contaminated after an accident.

Interdisciplinary Round-Robin Test on Molecular Spectroscopy of the U(VI) Acetate System.

A comprehensive molecular analysis of a simple aqueous complexing system-U(VI) acetate-selected to be independently investigated by various spectroscopic (vibrational, luminescence, X-ray absorption, and nuclear magnetic resonance spectroscopy) and quantum chemical methods was achieved by an international round-robin test (RRT). Twenty laboratories from six different countries with a focus on actinide or geochemical research participated and contributed to this scientific endeavor. The outcomes of this RRT were considered on two levels of complexity: first, within each technical discipline, conformities as well as discrepancies of the results and their sources were evaluated.

Formation of CeSiO from cerium(iii) silicate precursors.

Although the preparation of CeSiO4 has been already reported, the formation of pure cerium silicate from aqueous precursors appears as a challenge. An innovative way of synthesis has been identified in this study, allowing the formation of CeSiO4 after hydrothermal treatment starting from Ce(iii) silicate precursors. Among the experimental parameters examined, significant effects were found according to the nature of the precursor and of the reactive media considered, the pH of the reactive media and the temperature of the hydrothermal process.

Probing the existence of uranyl trisulfate structures in the AMEX solvent extraction process.

Knowledge of the complex microstructure in solvent extraction phases is mandatory for a full comprehension of ionic separation. Coupling EXAFS with MD simulations for uranyl extraction in sulfuric media with tertiary amine extractants enabled unravelling of the unprecedented uranyl tri-sulfate structure.