[The study of the raw milk contamination by bacteria of the genus Campylobacter using traditional microbiological methods and quantitative PCR assay].

Gastroenterocolitis caused by Campylobacter bacteria are the most common acute infectious zoonotic foodborne diseases. One of the important factors for the transmission of infection is contaminated dairy products, so the assessment of contamination of raw milk with Campylobacter is necessary to develop effective measures to suppress the growth of the pathogen and ensure the safety of products. of the study was to assess the microbial characteristics of raw milk samples and the nature of their contamination with thermophilic bacteria of the Campylobacter genus.

Growth and Persistence of Campylobacter jejuni in Foodstuffs.

Campylobacter genus bacteria causing campylobacteriasis are difficult to culture. This fact necessitates creation of special approaches to studies of the behavior of these pathogens during the manufacture and storage of foodstuffs. The regularities of Campylobacter jejuni transition into an uncultivable state are studied under conditions simulating the process of immersion cooling of fresh poultry products.

[Optimization of microbiological methods for food control based on the differential-diagnostic media for the isolation and cultivation of bacteria of the genus ].

А screening study on the detection of campylobacteria in raw food products, semi-finished products and objects in the external environment in the poultry processing industry was conducted. The highest level of detection of campylobacteria is set for raw poultry products, including carcasses of broilers, turkeys and quail. A general accordance of getting Campylobacter in raw materials and food products with inadequate sanitary treatment of separate areas of production has been established: in most cases Campylobacter spp.

[Subacute oral toxicity of multi-walled carbon nanotubes].

Multiwall carbon nanotubes (MWCNTs) are regarded as environmental pollutants with increased risk. Recently MWCNTs have attracted attention as a promising component of packaging materials for food products, as carriers for agricultural plant growth stimulants, agrochemicals components and advanced pesticides, which creates the possibility of their exposure through the gastrointestinal tract. Objective of the research is assessment of sub-acute oral toxicity to rats of MWСNTs in an experiment lasting 100 days.

[Antibiotic resistance of strains isolated from food products].

Campylobacter jejuni is a leading member of the genus Campylobacter which cause up to 90% of laboratory confirmed cases of campylobacteriosis. The most important characteristic defining the biological features of C. jejuni is their sensitivity to antibiotics.

A diffusion model for the coordination of DNA replication in Schizosaccharomyces pombe.

The locations of proteins and epigenetic marks on the chromosomal DNA sequence are believed to demarcate the eukaryotic genome into distinct structural and functional domains that contribute to gene regulation and genome organization. However, how these proteins and epigenetic marks are organized in three dimensions remains unknown. Recent advances in proximity-ligation methodologies and high resolution microscopy have begun to expand our understanding of these spatial relationships.

[The study of the contamination and the levels of Campylobacter spp. during the processing of selected types of foods].

The purpose of the work was to study the nature of the Campylobacter spp. contamination during the processing of food products of plant and animal origin (raw poultry and beef meat, raw milk, leafy salads, sliced raw vegetables). In the study of 148 samples 50 strains of Campylobacter spp.

[Changes in the phase variant spectra in the populations of lactic acid bacteria under antibiotic treatment].

Effect of the antibiotics kanamycin and ampicillin on growth and phase variation of the populations of four strains of lactic acid bacteria (Lactobacillus sp. M67AT, L. casei MB, Enterococcus faecium M, and E.

Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure.

Successful progression through the cell cycle requires spatial and temporal regulation of gene transcript levels and the number, positions and condensation levels of chromosomes. Here we present a high resolution survey of genome interactions in Schizosaccharomyces pombe using synchronized cells to investigate cell cycle dependent changes in genome organization and transcription. Cell cycle dependent interactions were captured between and within S.

The statistical-mechanics of chromosome conformation capture.

Since Jacob and Monod's characterization of the role of DNA elements in gene control, it has been recognized that the linear organization of genome structure is important for the regulation of gene transcription and hence the manifestation of phenotypes. Similarly, it has long been hypothesized that the spatial organization (in three dimensions evolving through time), as part of the epigenome, makes a significant contribution to the genotype-phenotype transition. Proximity ligation assays commonly known as chromosome conformation capture (3C) and 3C based methodologies (e.

Effects of spin transitions degeneracy in pulsed EPR of the fullerene C70 triplet state produced by continuous light illumination.

X-band echo-detected electron paramagnetic resonance (ED EPR) spectra of triplet state of fullerene C(70) generated by continuous light illumination were found to correspond below 30K to a non-equilibrium electron spin polarization. Above 30K spectra are characteristic of Boltzmann equilibrium. Spectra were simulated fairly well with zero-field splitting parameters D=153 MHz and E and distributed within the range of 6-42 MHz.

[Nebivolol in the treatment of ischemic heart disease patients with chronic heart failure].

Aim: To assess effectiveness of inclusion of nebivolol in complex therapy of patients with chronic heart failure (CHF) due to ischemic heart disease (IHD).

Material: Patients (n=62, age 47-73 years) with NYHA class II-III CHF and left ventricular (LV) ejection fraction (EF) less than 45% receiving standard therapy +/- nebivolol (5 mg/day). The patients were followed up for 4 months.

[Mechanisms of hemopoiesis inhibition and recovery after the administration of carminomycin and rubomycin in an experiment].

The experiments on 766 albino non-inbred rats and 270 mice BALB/c showed that when there were no basic differences in the mechanism of the anthracycline myeloinhibitory effect associated with a decrease in the number of the exogenic trunk hemopoietic cells, inhibition of the bone marrow proliferative activity and a decreased rate of the hemopoietic elements differentiation, carminomycin induced a more pronounced and persisting impairment of the hematopoietic tissue proliferation, as compared to rubomycin on their administration in doses equivalent by the total toxicity.

[Effect of carminomycin on bone marrow cell proliferative activity and differentiation rate].

Autoradiographic investigations with the use of 3H-thymidine were performed on 140 noninbred albino rats. The fate of the label in the cells of the myeloid series was followed in the experimental and control groups. The animals of the experimental group were treated with carminomycin in the LD50 30 minutes after injecion of 3H-thymidine.

[Mechanism of the myeloinhibitory effect of carminomycin].

The proliferative activity and level of aberrant mitoses in the cells of the bone marrow were studied experimentally on 223 noninbred mice treated with carminomycin administered intraperitoneally in single (LD50) and repeated doses. When the antibiotic was used in a single dose the values of the mitotic activity of the bone marrow elements did not correspond to the severity of depression and thir quantitative composition, which was explained by an impairement of the mitosis quality and possible interkinetic destruction of a significant part of both erythroid and immature myeloid cells capable of division at early stages after the exposure. At the same time the level of the bone marrow devastation under conditions of the treatment with repeated doses was mainly determined by inhibition of the erythronormoblast proliferative activity.