Publications by authors named "Piatibrat L"

Changes in the number of individual cells and cellular complexes after a standard dispergation procedure were used as a criterion for evaluating the strength of the cellular contacts at various time-points after the irradiation of HeLa monolayers with a He-Ne laser (100 J/m2, 10 W/m2, 10 s). The per cent of cellular complexes increased after the irradiation, being maximal (19.5 +/- 0.

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Amount of ATP in HeLa cells in various phases of growth was measured after He-Ne laser irradiation (100 J/m2, 10 W/m2, 10 s) by a bioluminescent luciferin-luciferase method. In cells of the exponential phase of growth, the amount of ATP (basal level 8 x 10(-16) mole/cell) starts to increase in 15 min after the irradiation with a maximum (170% above the basal level) at 20 min, and then decreases gradually to the basal level. The sensitivity of the cells to He-Ne laser radiation is lowest in lag-phase of growth, then increases to a plateau (approximately 185% above the control level) from 5th day of cultivation.

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A monolayer of HeLa cells, at the stationary phase of growth, exposed to He-Ne laser radiation (632.8 nm; 100 J/m2) either 5 min or 60 min prior to gamma irradiation (0.1-10 Gy; 6.

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The combined effect of tetrasulfonated phthalocyanine (TSPC, 1.0 microM) or chloroaluminum phthalocyanine (CAPC, 1.0 microM) and copper vapour laser radiation (lambda = 670 nm) causes a dose dependent decrease in the survival rate of HeLa cells at exponential and stationary growth phases estimated by the trypan blue exclusion test or colony-forming ability test.

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A study was made of the lethal effect of pulse laser (second harmonic Nd+3:YAG laser of 532 nm, pulse length 3.3.10(-11) s, peak intensity from 4.

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A radiomodifying effect of the three types of laser radiation has been studied on HeLa cell culture. Low-intensity UV-radiation delivered as high repetition rate nanosecond pulses (the second harmonic of the copper vapours laser lambda = 271 nm) somewhat reinforces, and continuous low-intensity red radiation (He-Ne laser, lambda = 632.8 nm) of certain doses weakens the effect of 5 Gy gamma-radiation.

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The curves of relative growth rate of HeLa cells were studied after their irradiation at the stationary growth phase (He-Ne laser, lambda = 632.8 nm, D = 100 J/m2) and reinoculation to the fresh medium in different periods after the irradiation. The irradiation evokes changes in cells of the stationary phase which are realized in subcultivation.

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