Publications by authors named "Phyllis Gamble"
Objective: Epidemiological studies suggest that lactation is associated with long-term maternal health benefits. To avoid confounders in human studies, we used a previously characterized murine model to investigate the long-term effect of lactation on both cardiovascular function and adiposity.
Study Design: After the delivery of the pups, CD-1 female mice were randomly divided into two groups: lactated and nonlactated (NL).
Article Synopsis
- Recent studies have linked maternal acetaminophen (APAP) consumption to Attention Deficit Hyperactivity Disorder (ADHD) in children, but causality was not established.
- The researchers used pregnant mice to investigate whether APAP exposure leads to hyperkinetic behaviors in offspring by measuring locomotor activity and brain structure.
- Results showed no significant differences in movement or brain structure between APAP-exposed mice and controls, suggesting that APAP exposure during pregnancy does not cause hyperkinetic dysfunctions.
The objective of this study was to localize by neuroimaging the altered structural brain development of these offspring using an autism model of transgenic mice lacking contactin-associated protein-like 2 (Cntnap2). Pregnant dams were randomly allocated to fructose solution (10% W/V) as only drinking fluid or water. Cntnap2 heterozygous (+/-) offspring from each group were euthanized at 6 months of age and their whole brains evaluated by magnetic resonance imaging.
View Article and Find Full Text PDFBackground: Consumption of fructose-rich diets in the United States is on the rise and thought to be associated with obesity and cardiometabolic diseases.
Objective: We sought to determine the effects of antenatal exposure to high-fructose diet on offspring's development of metabolic syndrome-like phenotype and other cardiovascular disease risk factors later in life.
Study Design: Pregnant C57BL/6J dams were randomly allocated to fructose solution (10% wt/vol, n = 10) or water (n = 10) as the only drinking fluid from day 1 of pregnancy until delivery.
Objective: Using an animal model, we have previously shown that preeclampsia results in long-term adverse neuromotor outcomes in the offspring, and this phenotype was prevented by antenatal treatment with pravastatin. This study aims to localize the altered neuromotor programming in this animal model and to evaluate the role of pravastatin in its prevention.
Materials And Methods: For the preeclampsia model, pregnant CD-1 mice were randomly allocated to injection of adenovirus carrying sFlt-1 or its control virus carrying mFc into the tail vein.
Objective: Preeclampsia alters fetal programming and results in long-term metabolic consequences in the offspring. Pravastatin has been shown to prevent preeclampsia in animal models. Our aim was to characterize the effects of preeclampsia on fetal programming of adult growth and metabolic function, and evaluate the role of preventive pravastatin therapy, using a well characterized murine model.
View Article and Find Full Text PDFObjective: The objective of the study was to determine whether perinatal nicotine exposure adversely affects cardiovascular health in adulthood.
Study Design: C57Bl/6J female mice were randomized to 200 μg/mL nicotine in 2% saccharin or 2% saccharin alone from 2 weeks before breeding until weaning. Offspring weight, vital signs, and carotid artery vascular reactivity were studied.
Objective: We sought to establish a model of fetal programming of metabolic syndrome by exposure to soluble fms-like tyrosine kinase-1 (sFlt1)-induced preeclampsia (PE) and preexisting maternal obesity (MO).
Study Design: CD-1 female mice were placed on either standard or high-fat diet for 3 months. On day 8 of pregnancy, mice were injected with either adenovirus-carrying sFlt1 or adenovirus-carrying murine immunoglobulin G2α Fc fragment.
Objective: The purpose of this study was to determine whether fetal programming of adult blood pressure is altered in a previously characterized mouse model of preeclampsia that was induced by sFlt-1.
Study Design: CD-1 mouse mothers at day 8 of gestation were injected with an adenovirus carrying Flt 1-3 (10(9) plaque-forming units) or with an adenovirus carrying mFc as control (10(9) plaque-forming units). The resulting pups were followed until 6 months of age, at which time blood pressure (BP) was recorded continuously for 6 days.
Objective: The objective of the study was to investigate the effect of fetal programming on the development of atherosclerosis in the offspring in a mouse model.
Study Design: Male and female mice of the wild type and the knockout for the apoprotein E (apoE) gene were cross-bred to obtain all 4 possible genetic offspring types. The offspring were kept on regular chow and killed at 8 months of age.