In vitro and in silico anti-inflammatory effect of minor constituents from the roots of Buch.-Ham. ex D. Don.

Vietnamese traditional medicine has utilised the roots of Buch.-Ham. ex D.

Anti-Inflammatory Activity of Labdane and Norlabdane Diterpenoids from Leonurus sibiricus Related to Modulation of MAPKs Signaling Pathway.

, a widely cultivated herbaceous plant in Asian countries, exhibits diverse medicinal applications. Recent studies emphasize its pharmacological properties and efficacy in promoting bone health. In addition to the known compounds and their pharmacological activities, in this study, we isolated and elucidated two new labdane-type diterpenoids, (3,5,6,10)-3,6-dihydroxy-15-ethoxy-7-oxolabden-8(9),13(14)-dien-15,16-olide (1: ) and (4,5,10)-18-hydroxy-14,15-bisnorlabda-8-en-7,13-dione (2: ), a new natural phenolic compound, and a known compound from using advanced spectroscopic techniques, including circular dichroism spectroscopy, high-resolution mass spectrometry, and 1- and 2-dimensional NMR.

AMPK-Mediated Hypolipidemic Effects of a and Mixed Extract on High-Fat Diet-Induced Liver Triglyceride Accumulation: An In Vivo and In Vitro Study.

Background: This study investigates the hypolipidemic effects of a mixed extract of and (USCP119) in HFD-fed hamsters and in vitro cellular models.

Methods: Over an 8-week period, HFD-fed hamsters were assigned to one of six groups: normal diet, HFD control, HFD with 50 mg/kg USCP119, HFD with 100 mg/kg USCP119, HFD with 50 mg/kg USCP119 twice daily (BID), and HFD with omega-3 fatty acids. Key outcomes assessed included body weight, serum triglycerides (TG), total cholesterol (TC), liver weight, hepatic TG levels, and epididymal fat.

Inhibition of Soluble Epoxide Hydrolase by Cembranoid Diterpenes from Soft Coral : Enzyme Kinetics, Molecular Docking, and Molecular Dynamics.

Soluble epoxide hydrolase (sEH) is essential for converting epoxy fatty acids, such as epoxyeicosatrienoic acids (EETs), into their dihydroxy forms. EETs play a crucial role in regulating blood pressure, mediating anti-inflammatory responses, and modulating pain, making sEH a key target for therapeutic interventions. Current research is increasingly focused on identifying sEH inhibitors from natural sources, particularly marine environments, which are rich in bioactive compounds due to their unique metabolic adaptations.

Two New Steroidal Saponins with Potential Anti-Inflammatory Effects from the Aerial Parts of Markgr.

Markgr., a member of the Gnetaceae family, is distributed in Vietnam. This plant remains a botanical enigma with an unexplored diversity of chemical constituents and pharmacological effects.

Assessing the Efficacy of Acanthoic Acid Isolated from Nakai in Male Infertility: An In Vivo and In Silico Approach.

Acanthoic acid, a diterpene isolated from the root bark of Nakai, possesses diverse pharmacological activities, including anti-inflammatory, anti-diabetic, gastrointestinal protection, and cardiovascular protection. This study is the first to investigate the egg-hatching rates of affected by acanthoic acid. Notably, male flies supplemented with 10 μM acanthoic acid exhibited a strong increase in hatching rates compared with controls under adverse temperature conditions, suggesting a potential protective effect against environmental stressors.

Traditional Uses, Phytochemistry, and Pharmacological Activities of (L.) Less.: An Updated Review.

(L.) Less. is a perennial herbaceous plant found mainly in tropical areas, particularly in Southeast Asia, South America, and India.

Insights into the inhibitory activities of neolignans and diarylnonanoid derivatives from nutmeg ( Houtt.) seeds on soluble epoxide hydrolase using and approaches.

Two new neolignans, myrifralignans F-G ( and ), four new diarylnonanoid derivatives, myrifragranones A-D (-), and 18 known compounds were isolated and structurally elucidated from nutmeg ( Houtt.) seeds. The absolute configurations of these secondary metabolites were determined using the electronic circular dichroism technique.

Optimization of Ultrasonic-Assisted Extraction of α-Glucosidase Inhibitors from Using Artificial Neural Network and Response Surface Methodology.

Nakai is a plant with significant medicinal properties, such as anticancer, antioxidant, and anti-inflammatory activities, making it an attractive research target. Our study describes the isolation of major metabolites from , and their inhibitory activities on α-glucosidase were evaluated for the first time. The results revealed that nortrisflavaspidic acid ABB () is the most potent α-glucosidase inhibitor, with an IC of 34.

Inhibitory Activity of Bioactive Phloroglucinols from the Rhizomes of Dryopteris crassirhizoma on Escherichia coli β-Glucuronidase: Kinetic Analysis and Molecular Docking Studies.

Phloroglucinols-one of the major secondary metabolites in -exhibit various pharmacological effects, such as antiviral, antioxidant, and antidiabetic activities. This study evaluated 30 phloroglucinols isolated from the rhizomes of for their inhibitory activity on -glucuronidase via in vitro assays. Among them, dimeric phloroglucinols - moderately inhibited -glucuronidase, and trimeric phloroglucinols - showed strong inhibitory effects, with IC values ranging from 5.

Steroid glycosides isolated from Paris polyphylla var. chinensis aerial parts and paris saponin II induces G1/S-phase MCF-7 cell cycle arrest.

In our previous research on Vietnamese medicinal plants, we found that the ethanolic extract of the aerial parts of Paris polyphylla var. chinensis exhibited cytotoxic effects in vitro in the MCF-7 human cancer cell line. Here, we used combined chromatographic separations to isolate six compounds including a new steroid glycoside, paripoloside A (3), and five known compounds, from the butanol extract of the aerial parts of P.

Isolation, structural elucidation and molecular docking studies against SARS-CoV-2 main protease of new stigmastane-type steroidal glucosides isolated from the whole plants of .

Phytochemical investigation of the whole plants of Hance. led in the isolation and identification of two new stigmastane-type steroidal glucosides (-), namely vernogratiosides A (), and B (). Their chemical structures were fully elucidated based on 1 D/2D NMR spectroscopic, HR-ESI-MS data analyses, and by producing derivatives by chemical reactions.

SARS-CoV-2 main protease and papain-like protease inhibition by abietane-type diterpenes isolated from the branches of using molecular docking studies.

Using various chromatographic methods, five abietane-type diterpenes were isolated from the branches of for the first time. The chemical structures of the isolates were identified by modern spectroscopic techniques, including H and C nuclear magnetic resonance spectroscopy and by comparison with the literature. In addition, the binding potential of the isolated compounds to replicase protein, SARS-CoV-2 main protease and papain-like protease, were examined using molecular docking studies.

Aplydactylonins A-C, three new sesquiterpenes from the Vietnamese sea hare Aplysia dactylomela and their cytotoxicity.

Aplydactylonins A-C (1-3), three new sesquiterpenes, were isolated from the Vietnamese sea hare Aplysia dactylomela. Their structures and absolute configurations were elucidated based on spectroscopic analysis, X-ray crystallography, and density functional theory (DFT) calculations of NMR and ECD data. Compound 2 exhibited cytotoxicity against HepG2, DU145 and A549 cells with respective IC values of 4.

PTP1B inhibition studies of biological active phloroglucinols from the rhizomes of Dryopteris crassirhizoma: Kinetic properties and molecular docking simulation.

By various chromatographic methods, 30 phloroglucinols (1-30) were isolated from a methanol extract of Dryopteris crassirhizoma, including two new dimeric phloroglucinols (13 and 25). The structures of the isolates were confirmed by HR-MS, 1D, and 2D NMR as well as by comparison with the literature. The protein tyrosine phosphatase 1B (PTP1B) effects of the isolated compounds (1-30) were evaluated using sodium orthovanadate and ursolic acid as a positive control.

Inhibitory Effects of Cucurbitane-Type Triterpenoids from Fruit on Lipopolysaccharide-Stimulated Pro-Inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells.

The bitter melon, L., was once an important food and medicinal herb. Various studies have focused on the potential treatment of stomach disease with .

Secondary metabolites from a peanut-associated fungus IMBC-NMTP01 with cytotoxic, anti-inflammatory, and antimicrobial activities.

Chemical investigation of a peanut-associated fungal strain IMBC-NMTP01 resulted in isolation and identification of 14 secondary metabolites, including two new, -aspergillusol () and aspernigin (), and 12 known compounds: pyrophen (), 2-(hydroxyimino)-3-(4-hydroxyphenyl)propanoic acid (), aspergillusol A (), rubrofusarin B (), nigerasperone A (), fonsecin (), TMC-256C1 (), pyranonigrin A (), orlandin (), nigerasperone C (), asperpyrone A (), and 5-(hydroxymethyl)-2-furancarboxylic acid (). Compounds , showed cytotoxicity toward all six human cancer cell lines, including HepG2, KB, HL-60, MCF-7, SK-Mel2, and LNCaP, with IC values ranging from 8.4 to 84.

Bioactive Compounds from and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Pro-inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells.

The roots of Wild (Polygalaceae), which is among the most important components of traditional Chinese herbal medicine, have been widely used for over 1000 years to treat a variety of diseases. In the current investigation of secondary metabolites with anti-inflammatory properties from Korean medicinal plants, a phytochemical constituent study led to the isolation of 15 compounds (-) from the roots of via a combination of chromatographic methods. Their structures were determined by means of spectroscopic data such as nuclear magnetic resonance (NMR), 1D- and 2D-NMR, and liquid chromatography-mass spectrometry (LC-MS).

Enhancement of an In Vivo Anti-Inflammatory Activity of Oleanolic Acid through Glycosylation Occurring Naturally in .

(Lardizabalaceae) has been used as a traditional herbal medicine in Korea and China for its anti-inflammatory and analgesic properties. As part of a bioprospecting program aimed at the discovery of new bioactive compounds from Korean medicinal plants, a phytochemical study of leaves was carried out leading to isolation of two oleanane-type triterpene saponins, 3--[-d-glucopyranosyl (1→2)--l-arabinopyranosyl] oleanolic acid-28--[-d-glucopyranosyl (1→6)--d-glucopyranosyl] ester () and 3---l-arabinopyranosyl oleanolic acid-28--[-d-glucopyranosyl (1→6)--d-glucopyranosyl] ester (). Their structures were established unambiguously by spectroscopic methods such as one- and two-dimensional nuclear magnetic resonance and infrared spectroscopies, high-resolution electrospray ionization mass spectrometry and chemical reactions.

Cytotoxic and immunomodulatory phenol derivatives from a marine sponge-derived fungus sp. VK12.

Chemical investigation of the marine-derived endophytic fungus sp. VK12 resulted in isolation and identification of a new compound, (3)-(3',5'-dihydroxyphenyl)butan-2-one () and five known ones: AGI-7 (), sescandelin (), sescandelin-B (), 4-hydroxybenzaldehyde (), and hydroxysydonic acid (). The absolute configuration of was determined by time-dependent density functional theory electronic circular dichroism, specific optical rotation, and NMR calculations.

The chemical constituents of ethanolic extract from leaves and their anti-inflammatory effects.

(Lardizabalaceae) is an important medicinal plant in Korea, Japan, and China. Its leaves are used to treat many diseases because of their analgesic, sedative, and diuretic effects; however, there are few reports on their chemical constituents and biological activities. This study divided an ethanol extract into dichloromethane (DCM), ethyl acetate (EtOAc), and water fractions.

Isolation, structural elucidation, and insights into the anti-inflammatory effects of triterpene saponins from the leaves of Stauntonia hexaphylla.

Using various chromatographic techniques, 23 triterpene saponins (1-23) were isolated from an ethanol extract of Stauntonia hexaphylla, including two new compounds (12 and 15). Their chemical structures were established by comprehensive spectroscopic methods such as 1D- and 2D-NMR, and HR-ESI-MS, and chemical reactions. The anti-inflammatory activities of the isolated saponins were determined using the nitric oxide (NO) assay.