Publications by authors named "Phiwe Zuma"
Development and validation of an LC-MS/MS method for quantifying total and unbound doravirine in human plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
December 2024
Article Synopsis
- A highly sensitive LC-MS/MS method was developed to measure total and unbound doravirine in plasma from patients in a clinical trial, utilizing a single calibration curve for both forms of the drug.
- The method involved protein precipitation for total doravirine extraction and ultrafiltration for unbound doravirine, employing specific chromatography techniques to ensure accurate analysis.
- Validation tests confirmed the method's precision and robustness against various sample conditions, meeting international standards for effective quantification of doravirine concentrations.
Validation and application of a quantitative LC-MS/MS assay for the analysis of first-line anti-tuberculosis drugs, rifabutin and their metabolites in human breast milk.
J Chromatogr B Analyt Technol Biomed Life Sci
November 2022
Breast milk is the preferred method of infant nutrition. Breastfeeding infants born to mothers treated for TB may be at risk of drug toxicity through breast milk exposure, or potentially be vulnerable to select for drug resistance with low level drug exposure. Except for isoniazid, the quantification of first-line TB drugs including rifabutin in breast milk has not been previously described and will provide much-needed insight to TB drug exposure in breastfeeding infants.
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