Publications by authors named "Phiri Azele"
In our previous study, we identified a Short Interspersed Nuclear Element Retrotransposon Insertion Polymorphism (SINE-RIP) within the 3' untranslated region (3'UTR) of the Phospholipase A2 Group XVI (PLA2G16) gene, which is essential in lipid metabolism. In this study, we confirmed the presence of this 280 bp SINE insertion and examined its distribution across ten distinct pig breeds using PCR and sequencing. Subsequently, RT-PCR was employed to determine its potential for co-transcription.
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- Homologous recombination in endogenous retroviruses (ERVs) creates solo-LTRs, leading to genetic variation across different genomes.
- The study identified an average of 5630 solo-LTRs in 21 genomes and developed a method to detect polymorphisms in pig genomes, resulting in 927 predicted solo-LTR sites, with 603 considered highly reliable.
- Analysis showed that many of these solo-LTR sites were associated with or near genes, with 156 sites used for population genetics, demonstrating their effectiveness in clustering different pig breeds.