Publications by authors named "Phiranuphon Meksiarun"
Tumour heterogeneity plays a large role in the response of tumour tissues to radiation therapy. Inherent biological, physical, and even dose deposition heterogeneity all play a role in the resultant observed response. We here implement the use of Haralick textural analysis to quantify the observed glycogen production response, as observed via Raman spectroscopic mapping, of tumours irradiated within a murine model.
View Article and Find Full Text PDFExternal beam radiotherapy is a common form of treatment for breast cancer. Among patients and across different breast cancer subtypes, the response to radiation is heterogeneous. Radiation-induced biochemical changes were examined by Raman spectroscopy using cell lines that represent a spectrum of human breast cancer.
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- The study explores the structure of lycopene aggregates using UV-vis and Raman spectroscopies, finding that J- and H-aggregates show significant energy shifts compared to the lycopene monomer in vitro.
- A linear relationship is established between the frequencies of Raman bands and the S → S transition energy, suggesting that different aggregate conformations are influenced by the effective conjugated C═C chain lengths within lycopene due to environmental effects.
- In vivo measurements in ripening tomato fruits reveal that lycopene, especially in the J-aggregate form, increases as chlorophyll decreases, and Raman imaging visualizes the heterogeneous distribution of these aggregates within the fruit.
Article Synopsis
- The study investigated methods for removing paraffin components from oral cancer tissue spectra using three multivariate analysis techniques: Independent Component Analysis (ICA), Partial Least Squares (PLS), and Independent Component - Partial Least Square (IC-PLS).
- All three methods effectively removed paraffin at similar levels, while Principal Component Analysis (PCA) failed to do so.
- However, PLS and IC-PLS were less damaging to the cancer tissue spectral integrity compared to ICA, paving the way for using paraffin-removed spectra to create Raman images for better diagnosis alongside traditional staining methods.
Subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and fat beneath the dermis layer were investigated using a ball lens top hollow optical fiber Raman probe (BHRP). Hamsters were fed with trilinolein (TL) and tricaprin (TC) for six weeks and measurements were carried out every two weeks. The BHRP with an 800 μm diameter fused-silica ball lens was able to obtain information on the subcutaneous fat in a totally non-invasive manner.
View Article and Find Full Text PDFRaman spectroscopy has previously been applied for studying lipid metabolism. In this study, a ball lens-installed hollow optical fiber Raman probe (BHRP) was used for the noninvasive measurement of skin lipids in hamsters. Our analysis suggested that multi-unsaturated lipids, once converted into a structure containing conjugated double bonds, were oxidized to form peroxides.
View Article and Find Full Text PDFAn in vivo non-invasive quantitative analysis technique was introduced for evaluating the fat composition of living marine diatoms by using Raman spectroscopy in conjunction with a chemometric method. This technique enabled the observation of real-time variations in individual lipids in diatom cells without specific treatment or fat extraction. A confocal Raman spectroscope was used to measure the marine centric diatom Thalassiosira (T.
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